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Literature summary extracted from

  • Anishkin, A.; Vanegas, J.M.; Rogers, D.M.; Lorenzi, P.L.; Chan, W.K.; Purwaha, P.; Weinstein, J.N.; Sukharev, S.; Rempe, S.B.
    Catalytic role of the substrate defines specificity of therapeutic L-asparaginase (2015), J. Mol. Biol., 427, 2867-2885 .
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.1.1 the aspartate product in the crystal structure of L-ASP exists in an unusual alpha-COOH protonation state. The crystal structures may represent intermediate steps rather than initial binding. The substrate's alpha-carboxyl may serve as a proton acceptor and activate one of the catalytic threonines during L-ASP's nucleophilic attack on the amide carbon Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.1 0.018
-
 L-asparagine K0.5 value, Hill coefficient 1.5, pH 8.0, 37°C Escherichia coli
3.5.1.1 0.021
-
 L-asparagine K0.5 value, Hill coefficient 1.4, presence of 0.3 mM Gln, pH 8.0, 37°C Escherichia coli
3.5.1.1 0.025
-
 L-asparagine K0.5 value, Hill coefficient 1.4, presence of 0.9 mM Gln, pH 8.0, 37°C Escherichia coli
3.5.1.1 0.068
-
 L-asparagine K0.5 value, Hill coefficient 1.0, presence of 8 mM Gln, pH 8.0, 37°C Escherichia coli
3.5.1.1 1.6
-
 L-glutamine K0.5 value, Hill coefficient 1.1, pH 8.0, 37°C Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Escherichia coli P00805
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 L-asparagine + H2O
-
 Escherichia coli L-aspartate + NH3
-
 ?
3.5.1.1 L-glutamine + H2O reaction of EC 3.5.1.2 Escherichia coli L-glutamate + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.1 AnsB
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.1 2.2
-
 L-glutamine pH 8.0, 37°C Escherichia coli
3.5.1.1 48
-
 L-asparagine presence of 8 mM Gln, pH 8.0, 37°C Escherichia coli
3.5.1.1 60
-
 L-asparagine pH 8.0, 37°C Escherichia coli
3.5.1.1 67
-
 L-asparagine presence of 0.9 mM Gln, pH 8.0, 37°C Escherichia coli
3.5.1.1 72
-
 L-asparagine presence of 0.3 mM Gln, pH 8.0, 37°C Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.1.1 710
-
 L-asparagine using K0.5 value, Hill coefficient 1.0, presence of 8 mM Gln, pH 8.0, 37°C Escherichia coli
3.5.1.1 1400
-
 L-glutamine using K0.5 value, Hill coefficient 1.1, pH 8.0, 37°C Escherichia coli
3.5.1.1 2700
-
 L-asparagine using K0.5 value, Hill coefficient 1.4, presence of 0.9 mM Gln, pH 8.0, 37°C Escherichia coli
3.5.1.1 3300
-
 L-asparagine using K0.5 value, Hill coefficient 1.5, pH 8.0, 37°C Escherichia coli
3.5.1.1 3400
-
 L-asparagine using K0.5 value, Hill coefficient 1.4, presence of 0.3 mM Gln, pH 8.0, 37°C Escherichia coli