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Literature summary extracted from

  • Sridharan, S.; Wang, L.; Brown, A.; Dover, L.; Kremer, L.; Besra, G.; Sacchettini, J.
    X-ray crystal structure of Mycobacterium tuberculosis beta-ketoacyl acyl carrier protein synthase II (mtKasB) (2007), J. Mol. Biol., 366, 469-480 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.294 expressed in Escherichia coli Rosetta (DE3) cells Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.294 in complex with palmitoyl-CoA, sitting drop vapor diffusion method, using 100 mM CAPS pH 10.5, 20 % w/v PEG 8000, 200 mM NaCl, spermine hydrochloride and foscholine-9 Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.294 88000
-
 gel filtration Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.294 an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] Mycobacterium tuberculosis
-
 an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
-
 ?
2.3.1.294 an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] Mycobacterium tuberculosis H37Rv
-
 an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.294 Mycobacterium tuberculosis
-
-
-
2.3.1.294 Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.294 Ni2+-charged HiTrap chelating agarose column chromatography and Superdex 200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.294 an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]
-
 Mycobacterium tuberculosis an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
-
 ?
2.3.1.294 an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]
-
 Mycobacterium tuberculosis H37Rv an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
-
 ?
2.3.1.294 palmitoyl-CoA + a malonyl-[acyl-carrier protein]
-
 Mycobacterium tuberculosis ?
-
 ?
2.3.1.294 palmitoyl-CoA + a malonyl-[acyl-carrier protein]
-
 Mycobacterium tuberculosis H37Rv ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.294 homodimer x-ray crystallography Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.3.1.294 beta-ketoacyl acyl carrier protein synthase II
-
 Mycobacterium tuberculosis
2.3.1.294 KasB
-
 Mycobacterium tuberculosis