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Literature summary extracted from

  • Jiang, Z.; Lv, X.; Liu, Y.; Shin, H.D.; Li, J.; Du, G.; Liu, L.
    Biocatalytic production of glucosamine from N-acetylglucosamine by diacetylchitobiose deacetylase (2018), J. Microbiol. Biotechnol., 28, 1850-1858 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.105 synthesis biocatalytic production of glucosamine from N-acetylglucosamine by diacetylchitobiose deacetylase. Although both strains are biocatalytically active, the performance of Bacillus subtilis is 2fold more effective. Establishment of an efficient biotransformation process for the biotechnological production of GlcN in Bacillus subtilis that is more environmentally friendly than the traditional multistep chemical synthesis approach. The overall optimal conditions are 18.6 g/l cells at 40°C, pH 7.5 for 3 h in 3-l bioreactor Pyrococcus horikoshii
3.5.1.136 synthesis a whole-cell biocatalytic process for the environment-friendly synthesis of glucosamine is developed. Biotechnological production of GlcN in Bacillus subtilis is more environmentally friendly than the traditional multistep chemical synthesis approach. It has great a potential for large-scale production of GlcN in an environment-friendly manner Pyrococcus furiosus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.105 gene PH0499, recombinant expression of the enzyme in Escherichia coli strain BL21(DE3) and in Bacillus subtilis strain WB600, subcloning in Escherichia coli strain JM109, method optimization, overview Pyrococcus horikoshii
3.5.1.136 expression in Bacillus subtilis WB600 is better than expression in Escherichia coli BL21(DE3) Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.105 N-acetyl-D-glucosamine + H2O Pyrococcus horikoshii
-
 D-glucosamine + acetate
-
 ?
3.5.1.105 N-acetyl-D-glucosamine + H2O Pyrococcus horikoshii DSM 12428
-
 D-glucosamine + acetate
-
 ?
3.5.1.105 N-acetyl-D-glucosamine + H2O Pyrococcus horikoshii NBRC 100139
-
 D-glucosamine + acetate
-
 ?
3.5.1.105 N-acetyl-D-glucosamine + H2O Pyrococcus horikoshii JCM 9974
-
 D-glucosamine + acetate
-
 ?
3.5.1.105 N-acetyl-D-glucosamine + H2O Pyrococcus horikoshii ATCC 700860
-
 D-glucosamine + acetate
-
 ?
3.5.1.105 N-acetyl-D-glucosamine + H2O Pyrococcus horikoshii OT-3
-
 D-glucosamine + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.105 Pyrococcus horikoshii O58235
-
-
3.5.1.105 Pyrococcus horikoshii ATCC 700860 O58235
-
-
3.5.1.105 Pyrococcus horikoshii DSM 12428 O58235
-
-
3.5.1.105 Pyrococcus horikoshii JCM 9974 O58235
-
-
3.5.1.105 Pyrococcus horikoshii NBRC 100139 O58235
-
-
3.5.1.105 Pyrococcus horikoshii OT-3 O58235
-
-
3.5.1.136 Pyrococcus furiosus Q8U3V1
-
-
3.5.1.136 Pyrococcus furiosus ATCC 700860 Q8U3V1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.105 N-acetyl-D-glucosamine + H2O
-
 Pyrococcus horikoshii D-glucosamine + acetate
-
 ?
3.5.1.105 N-acetyl-D-glucosamine + H2O
-
 Pyrococcus horikoshii DSM 12428 D-glucosamine + acetate
-
 ?
3.5.1.105 N-acetyl-D-glucosamine + H2O
-
 Pyrococcus horikoshii NBRC 100139 D-glucosamine + acetate
-
 ?
3.5.1.105 N-acetyl-D-glucosamine + H2O
-
 Pyrococcus horikoshii JCM 9974 D-glucosamine + acetate
-
 ?
3.5.1.105 N-acetyl-D-glucosamine + H2O
-
 Pyrococcus horikoshii ATCC 700860 D-glucosamine + acetate
-
 ?
3.5.1.105 N-acetyl-D-glucosamine + H2O
-
 Pyrococcus horikoshii OT-3 D-glucosamine + acetate
-
 ?
3.5.1.136 N-acetyl-D-glucosamine + H2O
-
 Pyrococcus furiosus beta-D-glucosamine + acetate
-
 ?
3.5.1.136 N-acetyl-D-glucosamine + H2O
-
 Pyrococcus furiosus ATCC 700860 beta-D-glucosamine + acetate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.105 Dacph
-
 Pyrococcus horikoshii
3.5.1.105 diacetylchitobiose deacetylase
-
 Pyrococcus horikoshii
3.5.1.105 PH0499
-
 Pyrococcus horikoshii
3.5.1.136 PH0499
-
 Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.105 80
-
 recombinant enzyme Pyrococcus horikoshii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.105 30 90 high activity at 40-90°C, 50% of maximal activity at 30°C Pyrococcus horikoshii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.105 7.5
-
 recombinant enzyme Pyrococcus horikoshii