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Literature summary extracted from

  • Abu-Tahon, M.A.; Isaac, G.S.
    Purification, characterization and anticancer efficiency of L-glutaminase from Aspergillus flavus (2019), J. Gen. Appl. Microbiol., 65, 284-292 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.2 medicine purified L-glutaminase has a high toxic effect on Hela and Hep G2 cell lines with an IC50 value 18 and 12 microg/ml, respectively, and a moderate cytotoxic effect on HCT-116 and MCF7 cells, with an IC50 value 44 and 58 microg/ml, respectively Aspergillus flavus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.2 Ca2+ 10 mM, 80% of initial activity Aspergillus flavus
3.5.1.2 Cu2+ 10 mM, 44% of initial activity Aspergillus flavus
3.5.1.2 Hg2+ 10 mM, 35% of initial activity Aspergillus flavus
3.5.1.2 iodoacetate 10 mM, 23% of initial activity Aspergillus flavus
3.5.1.2 Mg2+ 10 mM, 55% of initial activity Aspergillus flavus
3.5.1.2 N-ethylmaleimide 10 mM, 60% of initial activity Aspergillus flavus
3.5.1.2 Zn2+ 10 mM, 30% of initial activity Aspergillus flavus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.2 4.5
-
 L-glutamine pH 7.0, 30°C Aspergillus flavus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.2 Na+ 10 mM, 129% of initial activity Aspergillus flavus

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.2 Aspergillus flavus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.2
-
 Aspergillus flavus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.2 613.3
-
 pH 7.0, 30°C Aspergillus flavus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.2 L-albizziin + H2O
-
 Aspergillus flavus ? + NH3
-
 ?
3.5.1.2 L-asparagine + H2O
-
 Aspergillus flavus L-aspartate + NH3
-
 ?
3.5.1.2 L-glutamine + H2O
-
 Aspergillus flavus L-glutamate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.2 ? x * 69000, SDS-PAGE Aspergillus flavus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.2 40
-
-
 Aspergillus flavus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.2 8
-
-
 Aspergillus flavus