Literature summary extracted from

Stergiou, P.Y.; Foukis, A.; Gkini, O.A.; Bieth, J.G.; Papamichael, E.M.
Kinetic and computational analysis of the reversible inhibition of porcine pancreatic elastase a structural and mechanistic approach (2016), J. Enzyme Inhib. Med. Chem., 31, 131-139 .

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
3.4.21.36	N-(trifluoroacetyl)-alpha-aspartyl-N-[4-(trifluoromethyl)phenyl]alaninamide	reversible inhibitor	Sus scrofa
3.4.21.36	N-(trifluoroacetyl)leucyl-N-[4-(trifluoromethyl)phenyl]alaninamide	reversible inhibitor	Sus scrofa
3.4.21.36	N-(trifluoroacetyl)valyl-N-[4-(trifluoromethyl)phenyl]alaninamide	reversible inhibitor	Sus scrofa
3.4.21.36	N2-(trifluoroacetyl)lysyl-N-[4-(trifluoromethyl)phenyl]alaninamide	reversible inhibitor	Sus scrofa

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.4.21.36	0.504	-	succinyl-L-Ala-L-Ala-L-Ala-4-nitroanilide	at pH 8.0 and 25°C	Sus scrofa

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.4.21.36	Sus scrofa	-	-	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
3.4.21.36	pancreas	-	Sus scrofa	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.4.21.36	succinyl-L-Ala-L-Ala-L-Ala-4-nitroanilide + H2O	-	Sus scrofa	succinyl-L-Ala-L-Ala-L-Ala + 4-nitroaniline	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
3.4.21.36	pancreatic elastase	-	Sus scrofa
3.4.21.36	PPE	-	Sus scrofa

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.4.21.36	1.039	-	succinyl-L-Ala-L-Ala-L-Ala-4-nitroanilide	at pH 8.0 and 25°C	Sus scrofa

Ki Value [mM]

EC Number	Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
3.4.21.36	0.0000236	-	N-(trifluoroacetyl)leucyl-N-[4-(trifluoromethyl)phenyl]alaninamide	at pH 8.0 and 25°C	Sus scrofa

kcat/KM [mM/s]

EC Number	kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
3.4.21.36	2.06	-	succinyl-L-Ala-L-Ala-L-Ala-4-nitroanilide	at pH 8.0 and 25°C	Sus scrofa

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Release 2023.1 (January 2023)