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Literature summary extracted from
- Limonene dehydrogenase hydroxylates the allylic methyl group of cyclic monoterpenes in the anaerobic terpene degradation by Castellaniella defragrans (2018), J. Biol. Chem., 293, 9520-9529 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.17.99.8 | expression in Escherichia coli | Castellaniella defragrans |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.17.99.8 | 0.018 | - |
limonene | pH 8.0, 28°C | Castellaniella defragrans |  |
| 1.17.99.8 | 0.043 | - |
limonene | pH 8.0, 40°C | Castellaniella defragrans | |
| 1.17.99.8 | 0.392 | - |
perillyl alcohol | pH 8.0, 28°C | Castellaniella defragrans | |
| 1.17.99.8 | 0.487 | - |
perillyl alcohol | pH 8.0, 40°C | Castellaniella defragrans | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.17.99.8 | cytoplasm | - |
Castellaniella defragrans | 5737 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.17.99.8 | 152000 | - |
gel filtration | Castellaniella defragrans |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.17.99.8 | (R)-limonene + H2O + acceptor | Castellaniella defragrans | the in vivo electron acceptor may be a heterodimeric electron transfer flavoprotein (ETF) | (+)-perillyl alcohol + reduced acceptor | - |
? | |
| 1.17.99.8 | (R)-limonene + H2O + acceptor | Castellaniella defragrans 65Phen | the in vivo electron acceptor may be a heterodimeric electron transfer flavoprotein (ETF) | (+)-perillyl alcohol + reduced acceptor | - |
? | |
| 1.17.99.8 | (S)-limonene + H2O + acceptor | Castellaniella defragrans | the in vivo electron acceptor may be a heterodimeric electron transfer flavoprotein (ETF). The in vivo electron acceptor may be a heterodimeric electron transfer flavoprotein (ETF) | (-)-perillyl alcohol + reduced acceptor | - |
? | |
| 1.17.99.8 | (S)-limonene + H2O + acceptor | Castellaniella defragrans 65Phen | the in vivo electron acceptor may be a heterodimeric electron transfer flavoprotein (ETF). The in vivo electron acceptor may be a heterodimeric electron transfer flavoprotein (ETF) | (-)-perillyl alcohol + reduced acceptor | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.17.99.8 | Castellaniella defragrans | W8X9R6 AND W8X092 | W8X9R6: CtmA, W8X092: CtmB | - |
| 1.17.99.8 | Castellaniella defragrans 65Phen | W8X9R6 AND W8X092 | W8X9R6: CtmA, W8X092: CtmB | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.17.99.8 | - |
Castellaniella defragrans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.17.99.8 | (+)-p-menth-1-ene + H2O + ferrocenium hexafluorophosphate | - |
Castellaniella defragrans | [(4S)-4-(propan-2-yl)cyclohex-1-en-1-yl]methanol + reduced ferrocenium hexafluorophosphate | - |
? | |
| 1.17.99.8 | (+)-p-menth-1-ene + H2O + ferrocenium hexafluorophosphate | - |
Castellaniella defragrans 65Phen | [(4S)-4-(propan-2-yl)cyclohex-1-en-1-yl]methanol + reduced ferrocenium hexafluorophosphate | - |
? | |
| 1.17.99.8 | (R)-(-)-alpha-phellandrene + ferrocenium hexafluorophosphate | - |
Castellaniella defragrans | ? + reduced ferrocenium hexafluorophosphate | - |
? | |
| 1.17.99.8 | (R)-(-)-alpha-phellandrene + ferrocenium hexafluorophosphate | - |
Castellaniella defragrans 65Phen | ? + reduced ferrocenium hexafluorophosphate | - |
? | |
| 1.17.99.8 | (R)-limonene + H2O + acceptor | the in vivo electron acceptor may be a heterodimeric electron transfer flavoprotein (ETF) | Castellaniella defragrans | (+)-perillyl alcohol + reduced acceptor | - |
? | |
| 1.17.99.8 | (R)-limonene + H2O + acceptor | the in vivo electron acceptor may be a heterodimeric electron transfer flavoprotein (ETF) | Castellaniella defragrans 65Phen | (+)-perillyl alcohol + reduced acceptor | - |
? | |
| 1.17.99.8 | (R)-limonene + H2O + ferrocenium hexafluorophosphate | hydroxylates the R- and S-enantiomers at a similar rate | Castellaniella defragrans | (+)-perillyl alcohol + reduced ferrocenium hexafluorophosphate | - |
? | |
| 1.17.99.8 | (S)-limonene + H2O + acceptor | the in vivo electron acceptor may be a heterodimeric electron transfer flavoprotein (ETF). The in vivo electron acceptor may be a heterodimeric electron transfer flavoprotein (ETF) | Castellaniella defragrans | (-)-perillyl alcohol + reduced acceptor | - |
? | |
| 1.17.99.8 | (S)-limonene + H2O + acceptor | the in vivo electron acceptor may be a heterodimeric electron transfer flavoprotein (ETF). The in vivo electron acceptor may be a heterodimeric electron transfer flavoprotein (ETF) | Castellaniella defragrans 65Phen | (-)-perillyl alcohol + reduced acceptor | - |
? | |
| 1.17.99.8 | (S)-limonene + H2O + ferrocenium hexafluorophosphate | hydroxylates the R- and S-enantiomers at a similar rate | Castellaniella defragrans | (-)-perillyl alcohol + reduced ferrocenium hexafluorophosphate | - |
? | |
| 1.17.99.8 | alpha-terpinene + ferrocenium hexafluorophosphate | - |
Castellaniella defragrans | ? + reduced ferrocenium hexafluorophosphate | - |
? | |
| 1.17.99.8 | limonene + H2O + ferrocenium hexafluorophosphate | hydroxylates the R- and S-enantiomers at a similar rate | Castellaniella defragrans | perillyl alcohol + reduced ferrocenium hexafluorophosphate | - |
? | |
| 1.17.99.8 | additional information | the native enzyme (CtmAB) oxidizes a wide range of monocyclic monoterpenes containing the allylic methyl group motif (1-methyl-cyclohex-1-ene) | Castellaniella defragrans | ? | - |
? | |
| 1.17.99.8 | additional information | the native enzyme (CtmAB) oxidizes a wide range of monocyclic monoterpenes containing the allylic methyl group motif (1-methyl-cyclohex-1-ene) | Castellaniella defragrans 65Phen | ? | - |
? | |
| 1.17.99.8 | perillyl alcohol + dithiothreitol | - |
Castellaniella defragrans | limonene + H2O + dithiothreitol S-oxide | - |
? | |
| 1.17.99.8 | terpinolene + ferrocenium hexafluorophosphate | low activity | Castellaniella defragrans | ? + reduced ferrocenium hexafluorophosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.17.99.8 | heterodimer | 1 * 60700 (CtmB) + 1 * 61700 (CtmA), SDS-PAGE | Castellaniella defragrans |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.17.99.8 | ctmAB | - |
Castellaniella defragrans |
| 1.17.99.8 | limonene:ferrocenium oxidoreductase | - |
Castellaniella defragrans |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.17.99.8 | 40 | - |
- |
Castellaniella defragrans |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.17.99.8 | 7.5 | 8 | potassium phosphate buffer | Castellaniella defragrans |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.17.99.8 | FAD | each subunit (CtmA and CtmB) carries a flavin cofactor | Castellaniella defragrans | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.17.99.8 | 0.0053 | - |
perillyl alcohol | pH 8.0, 28°C | Castellaniella defragrans | |
| 1.17.99.8 | 0.0103 | - |
perillyl alcohol | pH 8.0, 40°C | Castellaniella defragrans | |
| 1.17.99.8 | 47 | - |
limonene | pH 8.0, 28°C | Castellaniella defragrans | |
| 1.17.99.8 | 63 | - |
limonene | pH 8.0, 40°C | Castellaniella defragrans | |
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