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Literature summary extracted from

  • Boros, E.; Szabo, A.; Zboray, K.; Heja, D.; Pal, G.; Sahin-Toth, M.
    Overlapping specificity of duplicated human pancreatic elastase 3 isoforms and archetypal porcine elastase 1 provides clues to evolution of digestive enzymes (2017), J. Biol. Chem., 292, 2690-2702 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.36 Trypsin
-
 Homo sapiens
3.4.21.36 Trypsin
-
 Sus scrofa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.36 expressed in HEK-293T cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.36 Schistocerca gregaria proteinase inhibitor 2 variant E1 Tyr-Cys-Thr-Leu-Met-Leu-Cys-His Homo sapiens
3.4.21.36 Schistocerca gregaria proteinase inhibitor 2 variant E1 Tyr-Cys-Thr-Leu-Met-Leu-Cys-His Sus scrofa
3.4.21.36 Schistocerca gregaria proteinase inhibitor 2 variant E10 Ala-Cys-Thr-Leu-Met-Leu-Cys-His Homo sapiens
3.4.21.36 Schistocerca gregaria proteinase inhibitor 2 variant E10 Ala-Cys-Thr-Leu-Met-Leu-Cys-His Sus scrofa
3.4.21.36 Schistocerca gregaria proteinase inhibitor 2 variant E11 Ala-Cys-Thr-Leu-Met-Tyr-Cys-His Homo sapiens
3.4.21.36 Schistocerca gregaria proteinase inhibitor 2 variant E11 Ala-Cys-Thr-Leu-Met-Tyr-Cys-His Sus scrofa
3.4.21.36 Schistocerca gregaria proteinase inhibitor 2 variant E12 Tyr-Cys-Thr-Leu-Met-Leu-Cys-Ala Homo sapiens
3.4.21.36 Schistocerca gregaria proteinase inhibitor 2 variant E12 Tyr-Cys-Thr-Leu-Met-Leu-Cys-Ala Sus scrofa
3.4.21.36 Schistocerca gregaria proteinase inhibitor 2 variant E2 Tyr-Cys-Thr-Ile-Met-Leu-Cys-His Homo sapiens
3.4.21.36 Schistocerca gregaria proteinase inhibitor 2 variant E2 Tyr-Cys-Thr-Ile-Met-Leu-Cys-His Sus scrofa
3.4.21.36 Schistocerca gregaria proteinase inhibitor 2 variant E3 Tyr-Cys-Thr-Val-Met-Leu-Cys-His Homo sapiens
3.4.21.36 Schistocerca gregaria proteinase inhibitor 2 variant E3 Tyr-Cys-Thr-Val-Met-Leu-Cys-His Sus scrofa
3.4.21.36 Schistocerca gregaria proteinase inhibitor 2 variant E4 Tyr-Cys-Thr-Met-Met-Leu-Cys-His Homo sapiens
3.4.21.36 Schistocerca gregaria proteinase inhibitor 2 variant E4 Tyr-Cys-Thr-Met-Met-Leu-Cys-His Sus scrofa
3.4.21.36 Schistocerca gregaria proteinase inhibitor 2 variant E5 Tyr-Cys-Thr-Ala-Met-Leu-Cys-His Homo sapiens
3.4.21.36 Schistocerca gregaria proteinase inhibitor 2 variant E5 Tyr-Cys-Thr-Ala-Met-Leu-Cys-His Sus scrofa
3.4.21.36 Schistocerca gregaria proteinase inhibitor 2 variant E6 Tyr-Cys-Thr-Ser-Met-Leu-Cys-His Homo sapiens
3.4.21.36 Schistocerca gregaria proteinase inhibitor 2 variant E6 Tyr-Cys-Thr-Ser-Met-Leu-Cys-His Sus scrofa
3.4.21.36 Schistocerca gregaria proteinase inhibitor 2 variant E7 Tyr-Cys-Thr-Ile-Met-Glu-Cys-His Homo sapiens
3.4.21.36 Schistocerca gregaria proteinase inhibitor 2 variant E7 Tyr-Cys-Thr-Ile-Met-Glu-Cys-His Sus scrofa
3.4.21.36 Schistocerca gregaria proteinase inhibitor 2 variant E8 Tyr-Cys-Thr-Leu-Arg-Leu-Cys-His Homo sapiens
3.4.21.36 Schistocerca gregaria proteinase inhibitor 2 variant E8 Tyr-Cys-Thr-Leu-Arg-Leu-Cys-His Sus scrofa
3.4.21.36 Schistocerca gregaria proteinase inhibitor 2 variant E9 Tyr-Cys-Thr-Leu-Met-Tyr-Cys-His Homo sapiens
3.4.21.36 Schistocerca gregaria proteinase inhibitor 2 variant E9 Tyr-Cys-Thr-Leu-Met-Tyr-Cys-His Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.36 0.166
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Ala-4-nitroanilide isoform CELA1, at pH 8.0 and 22°C Sus scrofa
3.4.21.36 0.259
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide isoform CELA3B, at pH 8.0 and 22°C Homo sapiens
3.4.21.36 0.288
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Met-4-nitroanilide isoform CELA3B, at pH 8.0 and 22°C Homo sapiens
3.4.21.36 0.374
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Ala-4-nitroanilide isoform CELA3B, at pH 8.0 and 22°C Homo sapiens
3.4.21.36 0.43
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Val-4-nitroanilide isoform CELA3B, at pH 8.0 and 22°C Homo sapiens
3.4.21.36 0.452
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Met-4-nitroanilide isoform CELA3A, at pH 8.0 and 22°C Homo sapiens
3.4.21.36 0.484
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Ile-4-nitroanilide isoform CELA3B, at pH 8.0 and 22°C Homo sapiens
3.4.21.36 0.54
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Met-4-nitroanilide isoform CELA1, at pH 8.0 and 22°C Sus scrofa
3.4.21.36 0.722
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide isoform CELA3A, at pH 8.0 and 22°C Homo sapiens
3.4.21.36 0.785
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide isoform CELA1, at pH 8.0 and 22°C Sus scrofa
3.4.21.36 0.984
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Ser-4-nitroanilide isoform CELA3B, at pH 8.0 and 22°C Homo sapiens
3.4.21.36 0.995
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Ile-4-nitroanilide isoform CELA1, at pH 8.0 and 22°C Sus scrofa
3.4.21.36 1.029
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Val-4-nitroanilide isoform CELA1, at pH 8.0 and 22°C Sus scrofa
3.4.21.36 1.06
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Ile-4-nitroanilide isoform CELA3A, at pH 8.0 and 22°C Homo sapiens
3.4.21.36 1.089
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Ala-4-nitroanilide isoform CELA3A, at pH 8.0 and 22°C Homo sapiens
3.4.21.36 1.178
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Val-4-nitroanilide isoform CELA3A, at pH 8.0 and 22°C Homo sapiens
3.4.21.36 1.447
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Ser-4-nitroanilide isoform CELA3A, at pH 8.0 and 22°C Homo sapiens
3.4.21.36 1.555
-
 succinyl-L-Ala-L-Ala-L-Pro-Gly-4-nitroanilide isoform CELA1, at pH 8.0 and 22°C Sus scrofa
3.4.21.36 2.311
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Ser-4-nitroanilide isoform CELA1, at pH 8.0 and 22°C Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.36 Homo sapiens
-
-
-
3.4.21.36 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.36 nickel-affinity chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.36 pancreas
-
 Homo sapiens
-
3.4.21.36 pancreas
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.36 succinyl-L-Ala-L-Ala-L-Pro-Gly-4-nitroanilide + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.36 succinyl-L-Ala-L-Ala-L-Pro-Gly-4-nitroanilide + H2O
-
 Sus scrofa ?
-
 ?
3.4.21.36 succinyl-L-Ala-L-Ala-L-Pro-L-Ala-4-nitroanilide + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.36 succinyl-L-Ala-L-Ala-L-Pro-L-Ala-4-nitroanilide + H2O
-
 Sus scrofa ?
-
 ?
3.4.21.36 succinyl-L-Ala-L-Ala-L-Pro-L-Ile-4-nitroanilide + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.36 succinyl-L-Ala-L-Ala-L-Pro-L-Ile-4-nitroanilide + H2O
-
 Sus scrofa ?
-
 ?
3.4.21.36 succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.36 succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide + H2O
-
 Sus scrofa ?
-
 ?
3.4.21.36 succinyl-L-Ala-L-Ala-L-Pro-L-Met-4-nitroanilide + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.36 succinyl-L-Ala-L-Ala-L-Pro-L-Met-4-nitroanilide + H2O
-
 Sus scrofa ?
-
 ?
3.4.21.36 succinyl-L-Ala-L-Ala-L-Pro-L-Ser-4-nitroanilide + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.36 succinyl-L-Ala-L-Ala-L-Pro-L-Ser-4-nitroanilide + H2O
-
 Sus scrofa ?
-
 ?
3.4.21.36 succinyl-L-Ala-L-Ala-L-Pro-L-Val-4-nitroanilide + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.36 succinyl-L-Ala-L-Ala-L-Pro-L-Val-4-nitroanilide + H2O
-
 Sus scrofa ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.36 CELA1
-
 Sus scrofa
3.4.21.36 CELA3A isoform Homo sapiens
3.4.21.36 CELA3B isoform Homo sapiens
3.4.21.36 chymotrypsin-like elastase
-
 Homo sapiens
3.4.21.36 chymotrypsin-like elastase
-
 Sus scrofa
3.4.21.36 elastase 1
-
 Sus scrofa
3.4.21.36 pancreatic elastase 3
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.36 0.14
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Ser-4-nitroanilide isoform CELA3A, at pH 8.0 and 22°C Homo sapiens
3.4.21.36 0.24
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Met-4-nitroanilide isoform CELA3A, at pH 8.0 and 22°C Homo sapiens
3.4.21.36 0.24
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Met-4-nitroanilide isoform CELA3B, at pH 8.0 and 22°C Homo sapiens
3.4.21.36 0.26
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Ser-4-nitroanilide isoform CELA3B, at pH 8.0 and 22°C Homo sapiens
3.4.21.36 0.7
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Met-4-nitroanilide isoform CELA1, at pH 8.0 and 22°C Sus scrofa
3.4.21.36 0.99
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Ile-4-nitroanilide isoform CELA3A, at pH 8.0 and 22°C Homo sapiens
3.4.21.36 1.01
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide isoform CELA3B, at pH 8.0 and 22°C Homo sapiens
3.4.21.36 1.16
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide isoform CELA3A, at pH 8.0 and 22°C Homo sapiens
3.4.21.36 1.25
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Ile-4-nitroanilide isoform CELA3B, at pH 8.0 and 22°C Homo sapiens
3.4.21.36 1.86
-
 succinyl-L-Ala-L-Ala-L-Pro-Gly-4-nitroanilide isoform CELA1, at pH 8.0 and 22°C Sus scrofa
3.4.21.36 2.44
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Val-4-nitroanilide isoform CELA3A, at pH 8.0 and 22°C Homo sapiens
3.4.21.36 2.87
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Ile-4-nitroanilide isoform CELA1, at pH 8.0 and 22°C Sus scrofa
3.4.21.36 3.66
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Val-4-nitroanilide isoform CELA3B, at pH 8.0 and 22°C Homo sapiens
3.4.21.36 5.62
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Ser-4-nitroanilide isoform CELA1, at pH 8.0 and 22°C Sus scrofa
3.4.21.36 16.9
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Val-4-nitroanilide isoform CELA1, at pH 8.0 and 22°C Sus scrofa
3.4.21.36 22.9
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Ala-4-nitroanilide isoform CELA3A, at pH 8.0 and 22°C Homo sapiens
3.4.21.36 24.8
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Ala-4-nitroanilide isoform CELA3B, at pH 8.0 and 22°C Homo sapiens
3.4.21.36 57.8
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide isoform CELA1, at pH 8.0 and 22°C Sus scrofa
3.4.21.36 115
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Ala-4-nitroanilide isoform CELA1, at pH 8.0 and 22°C Sus scrofa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.21.36 0.099
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Ser-4-nitroanilide isoform CELA3A, at pH 8.0 and 22°C Homo sapiens
3.4.21.36 0.26
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Ser-4-nitroanilide isoform CELA3B, at pH 8.0 and 22°C Homo sapiens
3.4.21.36 0.53
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Met-4-nitroanilide isoform CELA3A, at pH 8.0 and 22°C Homo sapiens
3.4.21.36 0.83
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Met-4-nitroanilide isoform CELA3B, at pH 8.0 and 22°C Homo sapiens
3.4.21.36 0.93
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Ile-4-nitroanilide isoform CELA3A, at pH 8.0 and 22°C Homo sapiens
3.4.21.36 1.2
-
 succinyl-L-Ala-L-Ala-L-Pro-Gly-4-nitroanilide isoform CELA1, at pH 8.0 and 22°C Sus scrofa
3.4.21.36 1.3
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Met-4-nitroanilide isoform CELA1, at pH 8.0 and 22°C Sus scrofa
3.4.21.36 1.6
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide isoform CELA3A, at pH 8.0 and 22°C Homo sapiens
3.4.21.36 2.1
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Val-4-nitroanilide isoform CELA3A, at pH 8.0 and 22°C Homo sapiens
3.4.21.36 2.4
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Ser-4-nitroanilide isoform CELA1, at pH 8.0 and 22°C Sus scrofa
3.4.21.36 2.6
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Ile-4-nitroanilide isoform CELA3B, at pH 8.0 and 22°C Homo sapiens
3.4.21.36 2.9
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Ile-4-nitroanilide isoform CELA1, at pH 8.0 and 22°C Sus scrofa
3.4.21.36 3.9
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide isoform CELA3B, at pH 8.0 and 22°C Homo sapiens
3.4.21.36 8.5
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Val-4-nitroanilide isoform CELA3B, at pH 8.0 and 22°C Homo sapiens
3.4.21.36 17
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Val-4-nitroanilide isoform CELA1, at pH 8.0 and 22°C Sus scrofa
3.4.21.36 21
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Ala-4-nitroanilide isoform CELA3A, at pH 8.0 and 22°C Homo sapiens
3.4.21.36 66
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Ala-4-nitroanilide isoform CELA3B, at pH 8.0 and 22°C Homo sapiens
3.4.21.36 74
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide isoform CELA1, at pH 8.0 and 22°C Sus scrofa
3.4.21.36 690
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Ala-4-nitroanilide isoform CELA1, at pH 8.0 and 22°C Sus scrofa