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Literature summary extracted from
- Purification and biochemical characterization of the Mycobacterium tuberculosis beta-ketoacyl-acyl carrier protein synthases KasA and KasB (2001), J. Biol. Chem., 276, 47029-47037 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.3.1.293 | expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
| 2.3.1.294 | expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.3.1.293 | cerulenin | 41-44% inhibition at 0.1 mM | Mycobacterium tuberculosis |  |
| 2.3.1.293 | thiolactomycin | 90-98% inhibition at 0.1 mM | Mycobacterium tuberculosis | |
| 2.3.1.294 | cerulenin | 37-41% inhibition at 0.1 mM | Mycobacterium tuberculosis | |
| 2.3.1.294 | thiolactomycin | 64-65% inhibition at 0.1 mM | Mycobacterium tuberculosis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.3.1.293 | 0.0025 | - |
C20:0-[acyl-carrier protein from Escherichia coli] | at pH 6.8 and 37°C | Mycobacterium tuberculosis | |
| 2.3.1.293 | 0.0032 | - |
palmitoyl-[acyl-carrier protein from Escherichia coli] | at pH 6.8 and 37°C | Mycobacterium tuberculosis | |
| 2.3.1.293 | 0.0135 | - |
malonyl-[acyl-carrier protein from Escherichia coli] | at pH 6.8 and 37°C | Mycobacterium tuberculosis | |
| 2.3.1.294 | 0.0014 | - |
palmitoyl-[acyl-carrier protein from Escherichia coli] | at pH 7.0 and 37°C | Mycobacterium tuberculosis | |
| 2.3.1.294 | 0.0019 | - |
C20:0-[acyl-carrier protein from Escherichia coli] | at pH 7.0 and 37°C | Mycobacterium tuberculosis | |
| 2.3.1.294 | 0.0135 | - |
malonyl-[acyl-carrier protein from Escherichia coli] | at pH 7.0 and 37°C | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.3.1.293 | an ultra-long-chain mono-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] | Mycobacterium tuberculosis | - |
an ultra-long-chain mono-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] | - |
? | |
| 2.3.1.293 | an ultra-long-chain mono-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] | Mycobacterium tuberculosis H37Rv | - |
an ultra-long-chain mono-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] | - |
? | |
| 2.3.1.294 | an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] | Mycobacterium tuberculosis | - |
an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.3.1.293 | Mycobacterium tuberculosis | - |
- |
- |
| 2.3.1.293 | Mycobacterium tuberculosis H37Rv | - |
- |
- |
| 2.3.1.294 | Mycobacterium tuberculosis | - |
- |
- |
| 2.3.1.294 | Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.3.1.293 | Q-Sepharose column chromatography and HiTrap column chromatography | Mycobacterium tuberculosis |
| 2.3.1.294 | Q-Sepharose column chromatography and HiTrap column chromatography | Mycobacterium tuberculosis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.3.1.293 | 0.0057 | - |
with palmitoyl-CoA as substrate, at pH 6.8 and 37°C | Mycobacterium tuberculosis |
| 2.3.1.293 | 0.0878 | - |
palmitoyl-[acyl-carrier protein] as substrate, at pH 6.8 and 37°C | Mycobacterium tuberculosis |
| 2.3.1.294 | 0.0034 | - |
with palmitoyl-CoA as substrate, at pH 7.0 and 37°C | Mycobacterium tuberculosis |
| 2.3.1.294 | 0.0318 | - |
palmitoyl-[acyl-carrier protein] as substrate, at pH 7.0 and 37°C | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.3.1.293 | an ultra-long-chain mono-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] | - |
Mycobacterium tuberculosis | an ultra-long-chain mono-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] | - |
? | |
| 2.3.1.293 | an ultra-long-chain mono-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] | - |
Mycobacterium tuberculosis H37Rv | an ultra-long-chain mono-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] | - |
? | |
| 2.3.1.293 | C12:0-[acyl-carrier protein from Escherichia coli] + malonyl-[acyl-carrier protein from Escherichia coli] | - |
Mycobacterium tuberculosis | ? | - |
? | |
| 2.3.1.293 | C12:0-[acyl-carrier protein from Escherichia coli] + malonyl-[acyl-carrier protein from Escherichia coli] | - |
Mycobacterium tuberculosis H37Rv | ? | - |
? | |
| 2.3.1.293 | C20:0-[acyl-carrier protein from Escherichia coli] + malonyl-[acyl-carrier protein from Escherichia coli] | best substrate | Mycobacterium tuberculosis | ? | - |
? | |
| 2.3.1.293 | C20:0-[acyl-carrier protein from Escherichia coli] + malonyl-[acyl-carrier protein from Escherichia coli] | best substrate | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
| 2.3.1.293 | C4:0-[acyl-carrier protein from Escherichia coli] + malonyl-[acyl-carrier protein from Escherichia coli] | very low activity | Mycobacterium tuberculosis | ? | - |
? | |
| 2.3.1.293 | C4:0-[acyl-carrier protein from Escherichia coli] + malonyl-[acyl-carrier protein from Escherichia coli] | very low activity | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
| 2.3.1.293 | palmitoyl-CoA + malonyl-[acyl-carrier protein from Escherichia coli] | weak activity | Mycobacterium tuberculosis | ? | - |
? | |
| 2.3.1.293 | palmitoyl-CoA + malonyl-[acyl-carrier protein from Escherichia coli] | weak activity | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
| 2.3.1.293 | palmitoyl-[acyl-carrier protein from Escherichia coli] + malonyl-[acyl-carrier protein from Escherichia coli] | - |
Mycobacterium tuberculosis | ? | - |
? | |
| 2.3.1.294 | an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] | - |
Mycobacterium tuberculosis | an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] | - |
? | |
| 2.3.1.294 | C12:0-[acyl-carrier protein from Escherichia coli] + malonyl-[acyl-carrier protein from Escherichia coli] | very low activity | Mycobacterium tuberculosis | ? | - |
? | |
| 2.3.1.294 | C20:0-[acyl-carrier protein from Escherichia coli] + malonyl-[acyl-carrier protein from Escherichia coli] | very low activity | Mycobacterium tuberculosis | ? | - |
? | |
| 2.3.1.294 | C4:0-[acyl-carrier protein from Escherichia coli] + malonyl-[acyl-carrier protein from Escherichia coli] | very low activity | Mycobacterium tuberculosis | ? | - |
? | |
| 2.3.1.294 | palmitoyl-CoA + malonyl-[acyl-carrier protein from Escherichia coli] | weak activity | Mycobacterium tuberculosis | ? | - |
? | |
| 2.3.1.294 | palmitoyl-[acyl-carrier protein from Escherichia coli] + malonyl-[acyl-carrier protein from Escherichia coli] | best substrate | Mycobacterium tuberculosis | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.3.1.293 | ? | x * 45000, SDS-PAGE | Mycobacterium tuberculosis |
| 2.3.1.294 | ? | x * 45000, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 2.3.1.293 | beta-ketoacyl-ACP synthase | - |
Mycobacterium tuberculosis |
| 2.3.1.293 | beta-ketoacyl-acyl carrier protein synthase | - |
Mycobacterium tuberculosis |
| 2.3.1.293 | KasA | - |
Mycobacterium tuberculosis |
| 2.3.1.294 | beta-ketoacyl-ACP synthase | - |
Mycobacterium tuberculosis |
| 2.3.1.294 | beta-ketoacyl-acyl carrier protein synthase | - |
Mycobacterium tuberculosis |
| 2.3.1.294 | KasB | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.3.1.293 | 37 | - |
- |
Mycobacterium tuberculosis |
| 2.3.1.294 | 37 | - |
- |
Mycobacterium tuberculosis |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.3.1.293 | 30 | 42 | the enzyme displays about 2-3fold higher specific activities at 37°C compared with 30 or 42°C | Mycobacterium tuberculosis |
| 2.3.1.294 | 30 | 42 | the enzyme displays about 2-3fold higher specific activities at 37°C compared with 30 or 42°C | Mycobacterium tuberculosis |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.3.1.293 | 0.075 | - |
C20:0-[acyl-carrier protein from Escherichia coli] | at pH 6.8 and 37°C | Mycobacterium tuberculosis | |
| 2.3.1.293 | 0.08 | - |
malonyl-[acyl-carrier protein from Escherichia coli] | at pH 6.8 and 37°C | Mycobacterium tuberculosis | |
| 2.3.1.293 | 0.088 | - |
palmitoyl-[acyl-carrier protein from Escherichia coli] | at pH 6.8 and 37°C | Mycobacterium tuberculosis | |
| 2.3.1.294 | 0.01 | - |
C20:0-[acyl-carrier protein from Escherichia coli] | at pH 7.0 and 37°C | Mycobacterium tuberculosis | |
| 2.3.1.294 | 0.023 | - |
malonyl-[acyl-carrier protein from Escherichia coli] | at pH 7.0 and 37°C | Mycobacterium tuberculosis | |
| 2.3.1.294 | 0.027 | - |
palmitoyl-[acyl-carrier protein from Escherichia coli] | at pH 7.0 and 37°C | Mycobacterium tuberculosis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.3.1.293 | NADPH | 0.05 mM NADPH is used in assay conditions | Mycobacterium tuberculosis | |
| 2.3.1.294 | NADPH | 0.05 mM NADPH is used in assay conditions | Mycobacterium tuberculosis | |
IC50 Value
| EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|---|
| 2.3.1.293 | 0.02 | - |
at pH 6.8 and 37°C | Mycobacterium tuberculosis | thiolactomycin | |
| 2.3.1.294 | 0.09 | - |
at pH 7.0 and 37°C | Mycobacterium tuberculosis | thiolactomycin | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.3.1.293 | 5.83 | - |
malonyl-[acyl-carrier protein from Escherichia coli] | at pH 6.8 and 37°C | Mycobacterium tuberculosis | |
| 2.3.1.293 | 28.33 | - |
palmitoyl-[acyl-carrier protein from Escherichia coli] | at pH 6.8 and 37°C | Mycobacterium tuberculosis | |
| 2.3.1.293 | 30 | - |
C20:0-[acyl-carrier protein from Escherichia coli] | at pH 6.8 and 37°C | Mycobacterium tuberculosis | |
| 2.3.1.294 | 1.67 | - |
malonyl-[acyl-carrier protein from Escherichia coli] | at pH 7.0 and 37°C | Mycobacterium tuberculosis | |
| 2.3.1.294 | 5.33 | - |
C20:0-[acyl-carrier protein from Escherichia coli] | at pH 7.0 and 37°C | Mycobacterium tuberculosis | |
| 2.3.1.294 | 18.33 | - |
palmitoyl-[acyl-carrier protein from Escherichia coli] | at pH 7.0 and 37°C | Mycobacterium tuberculosis |
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