EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.13.2.B1 | NAD+ | addition of excess NAD+ (0.2 mM) increases enzymatic activity by about 2fold | Methanocaldococcus jannaschii |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.13.1.9 | expression in Escherichia coli | Methanocaldococcus jannaschii |
3.13.2.B1 | gene MJ1388, sequence comparisons and phylogenetic analysis, recombinant expression of the enzyme in Escherichia coli strain BL21-CodonPlus(DE3)-RIL | Methanocaldococcus jannaschii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.13.1.9 | Inosine | above 2 mM | Methanocaldococcus jannaschii | |
3.13.2.B1 | additional information | the D-isomer present in the DL-homocysteine is not a strong inhibitor of SIHH | Methanocaldococcus jannaschii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.13.1.9 | 0.0054 | - |
L-homocysteine | pH 7.0, 70°C | Methanocaldococcus jannaschii | |
3.13.1.9 | 0.22 | - |
S-Inosyl-L-homocysteine | pH 7.0, 70°C | Methanocaldococcus jannaschii | |
3.13.1.9 | 0.64 | - |
Inosine | pH 7.0, 70°C | Methanocaldococcus jannaschii | |
3.13.2.B1 | 0.0054 | - |
L-homocysteine | pH 7.5, 70°C, recombinant enzyme | Methanocaldococcus jannaschii | |
3.13.2.B1 | 0.22 | - |
S-Inosyl-L-homocysteine | pH 7.5, 70°C, recombinant enzyme | Methanocaldococcus jannaschii | |
3.13.2.B1 | 0.64 | - |
Inosine | pH 7.5, 70°C, recombinant enzyme | Methanocaldococcus jannaschii |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.13.2.B1 | cytoplasm | - |
Methanocaldococcus jannaschii | 5737 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.13.1.9 | 49000 | - |
SDS-PAGE | Methanocaldococcus jannaschii |
3.13.2.B1 | 200000 | - |
about, gel filtration | Methanocaldococcus jannaschii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.13.1.9 | S-inosyl-L-homocysteine + H2O | Methanocaldococcus jannaschii | the enzyme participates in an alternative pathway for the regeneration of S-adenosyl-L-methionine from S-adenosyl-L-homocysteine that involves the deamination of the latter to S-inosyl-L-homocysteine | inosine + L-homocysteine | - |
? | |
3.13.1.9 | S-inosyl-L-homocysteine + H2O | Methanocaldococcus jannaschii NBRC 100440 | the enzyme participates in an alternative pathway for the regeneration of S-adenosyl-L-methionine from S-adenosyl-L-homocysteine that involves the deamination of the latter to S-inosyl-L-homocysteine | inosine + L-homocysteine | - |
? | |
3.13.1.9 | S-inosyl-L-homocysteine + H2O | Methanocaldococcus jannaschii DSM 2661 | the enzyme participates in an alternative pathway for the regeneration of S-adenosyl-L-methionine from S-adenosyl-L-homocysteine that involves the deamination of the latter to S-inosyl-L-homocysteine | inosine + L-homocysteine | - |
? | |
3.13.1.9 | S-inosyl-L-homocysteine + H2O | Methanocaldococcus jannaschii ATCC 43067 | the enzyme participates in an alternative pathway for the regeneration of S-adenosyl-L-methionine from S-adenosyl-L-homocysteine that involves the deamination of the latter to S-inosyl-L-homocysteine | inosine + L-homocysteine | - |
? | |
3.13.1.9 | S-inosyl-L-homocysteine + H2O | Methanocaldococcus jannaschii JAL-1 | the enzyme participates in an alternative pathway for the regeneration of S-adenosyl-L-methionine from S-adenosyl-L-homocysteine that involves the deamination of the latter to S-inosyl-L-homocysteine | inosine + L-homocysteine | - |
? | |
3.13.1.9 | S-inosyl-L-homocysteine + H2O | Methanocaldococcus jannaschii JCM 10045 | the enzyme participates in an alternative pathway for the regeneration of S-adenosyl-L-methionine from S-adenosyl-L-homocysteine that involves the deamination of the latter to S-inosyl-L-homocysteine | inosine + L-homocysteine | - |
? | |
3.13.2.B1 | S-inosyl-L-homocysteine + H2O | Methanocaldococcus jannaschii | - |
L-homocysteine + inosine | - |
r | |
3.13.2.B1 | S-inosyl-L-homocysteine + H2O | Methanocaldococcus jannaschii NBRC 100440 | - |
L-homocysteine + inosine | - |
r | |
3.13.2.B1 | S-inosyl-L-homocysteine + H2O | Methanocaldococcus jannaschii DSM 2661 | - |
L-homocysteine + inosine | - |
r | |
3.13.2.B1 | S-inosyl-L-homocysteine + H2O | Methanocaldococcus jannaschii ATCC 43067 | - |
L-homocysteine + inosine | - |
r | |
3.13.2.B1 | S-inosyl-L-homocysteine + H2O | Methanocaldococcus jannaschii JAL-1 | - |
L-homocysteine + inosine | - |
r | |
3.13.2.B1 | S-inosyl-L-homocysteine + H2O | Methanocaldococcus jannaschii JCM 10045 | - |
L-homocysteine + inosine | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.13.1.9 | Methanocaldococcus jannaschii | Q58783 | - |
- |
3.13.1.9 | Methanocaldococcus jannaschii ATCC 43067 | Q58783 | - |
- |
3.13.2.B1 | Methanocaldococcus jannaschii | Q58783 | - |
- |
3.13.2.B1 | Methanocaldococcus jannaschii ATCC 43067 | Q58783 | - |
- |
3.13.2.B1 | Methanocaldococcus jannaschii DSM 2661 | Q58783 | - |
- |
3.13.2.B1 | Methanocaldococcus jannaschii JAL-1 | Q58783 | - |
- |
3.13.2.B1 | Methanocaldococcus jannaschii JCM 10045 | Q58783 | - |
- |
3.13.2.B1 | Methanocaldococcus jannaschii NBRC 100440 | Q58783 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.13.1.9 | - |
Methanocaldococcus jannaschii |
3.13.2.B1 | recombinant enzyme from Escherichia coli strain BL21-CodonPlus(DE3)-RIL by heat treatment at 80°C for 10 min, and anion exchange chromatography | Methanocaldococcus jannaschii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.13.1.9 | inosine + L-homocysteine | the enzyme shows a slight preference for L-homocysteine in the synthesis direction | Methanocaldococcus jannaschii | S-inosyl-L-homocysteine + H2O | - |
r | |
3.13.1.9 | inosine + L-homocysteine | the enzyme shows a slight preference for L-homocysteine in the synthesis direction | Methanocaldococcus jannaschii NBRC 100440 | S-inosyl-L-homocysteine + H2O | - |
r | |
3.13.1.9 | inosine + L-homocysteine | the enzyme shows a slight preference for L-homocysteine in the synthesis direction | Methanocaldococcus jannaschii DSM 2661 | S-inosyl-L-homocysteine + H2O | - |
r | |
3.13.1.9 | inosine + L-homocysteine | the enzyme shows a slight preference for L-homocysteine in the synthesis direction | Methanocaldococcus jannaschii ATCC 43067 | S-inosyl-L-homocysteine + H2O | - |
r | |
3.13.1.9 | inosine + L-homocysteine | the enzyme shows a slight preference for L-homocysteine in the synthesis direction | Methanocaldococcus jannaschii JAL-1 | S-inosyl-L-homocysteine + H2O | - |
r | |
3.13.1.9 | inosine + L-homocysteine | the enzyme shows a slight preference for L-homocysteine in the synthesis direction | Methanocaldococcus jannaschii JCM 10045 | S-inosyl-L-homocysteine + H2O | - |
r | |
3.13.1.9 | S-inosyl-L-homocysteine + H2O | the enzyme participates in an alternative pathway for the regeneration of S-adenosyl-L-methionine from S-adenosyl-L-homocysteine that involves the deamination of the latter to S-inosyl-L-homocysteine | Methanocaldococcus jannaschii | inosine + L-homocysteine | - |
? | |
3.13.1.9 | S-inosyl-L-homocysteine + H2O | the enzyme is specific for the hydrolysis and synthesis of S-inosyl-L-homocysteine, not S-adenosyl-L-homocysteine | Methanocaldococcus jannaschii | inosine + L-homocysteine | - |
r | |
3.13.1.9 | S-inosyl-L-homocysteine + H2O | the enzyme participates in an alternative pathway for the regeneration of S-adenosyl-L-methionine from S-adenosyl-L-homocysteine that involves the deamination of the latter to S-inosyl-L-homocysteine | Methanocaldococcus jannaschii NBRC 100440 | inosine + L-homocysteine | - |
? | |
3.13.1.9 | S-inosyl-L-homocysteine + H2O | the enzyme is specific for the hydrolysis and synthesis of S-inosyl-L-homocysteine, not S-adenosyl-L-homocysteine | Methanocaldococcus jannaschii NBRC 100440 | inosine + L-homocysteine | - |
r | |
3.13.1.9 | S-inosyl-L-homocysteine + H2O | the enzyme participates in an alternative pathway for the regeneration of S-adenosyl-L-methionine from S-adenosyl-L-homocysteine that involves the deamination of the latter to S-inosyl-L-homocysteine | Methanocaldococcus jannaschii DSM 2661 | inosine + L-homocysteine | - |
? | |
3.13.1.9 | S-inosyl-L-homocysteine + H2O | the enzyme is specific for the hydrolysis and synthesis of S-inosyl-L-homocysteine, not S-adenosyl-L-homocysteine | Methanocaldococcus jannaschii DSM 2661 | inosine + L-homocysteine | - |
r | |
3.13.1.9 | S-inosyl-L-homocysteine + H2O | the enzyme participates in an alternative pathway for the regeneration of S-adenosyl-L-methionine from S-adenosyl-L-homocysteine that involves the deamination of the latter to S-inosyl-L-homocysteine | Methanocaldococcus jannaschii ATCC 43067 | inosine + L-homocysteine | - |
? | |
3.13.1.9 | S-inosyl-L-homocysteine + H2O | the enzyme is specific for the hydrolysis and synthesis of S-inosyl-L-homocysteine, not S-adenosyl-L-homocysteine | Methanocaldococcus jannaschii ATCC 43067 | inosine + L-homocysteine | - |
r | |
3.13.1.9 | S-inosyl-L-homocysteine + H2O | the enzyme participates in an alternative pathway for the regeneration of S-adenosyl-L-methionine from S-adenosyl-L-homocysteine that involves the deamination of the latter to S-inosyl-L-homocysteine | Methanocaldococcus jannaschii JAL-1 | inosine + L-homocysteine | - |
? | |
3.13.1.9 | S-inosyl-L-homocysteine + H2O | the enzyme is specific for the hydrolysis and synthesis of S-inosyl-L-homocysteine, not S-adenosyl-L-homocysteine | Methanocaldococcus jannaschii JAL-1 | inosine + L-homocysteine | - |
r | |
3.13.1.9 | S-inosyl-L-homocysteine + H2O | the enzyme participates in an alternative pathway for the regeneration of S-adenosyl-L-methionine from S-adenosyl-L-homocysteine that involves the deamination of the latter to S-inosyl-L-homocysteine | Methanocaldococcus jannaschii JCM 10045 | inosine + L-homocysteine | - |
? | |
3.13.1.9 | S-inosyl-L-homocysteine + H2O | the enzyme is specific for the hydrolysis and synthesis of S-inosyl-L-homocysteine, not S-adenosyl-L-homocysteine | Methanocaldococcus jannaschii JCM 10045 | inosine + L-homocysteine | - |
r | |
3.13.2.B1 | L-homocysteine + inosine | - |
Methanocaldococcus jannaschii | S-inosyl-L-homocysteine + H2O | - |
r | |
3.13.2.B1 | additional information | the annotated S-adenosyl-L-homocysteine hydrolase in Methanocaldococcus jannaschii is specific for the hydrolysis and synthesis of S-inosyl-L-homocysteine, not S-adenosyl-L-homocysteine. No enzymatic activity is detected with S-adenosyl-L-homocysteine as the substrate in either the synthesis or hydrolysis direction. Enzyme SIHH shows a slight preference for L-homocysteine in the synthesis direction. It retains 94% activity when assayed with the same concentration of DL-homocysteine | Methanocaldococcus jannaschii | ? | - |
? | |
3.13.2.B1 | additional information | the annotated S-adenosyl-L-homocysteine hydrolase in Methanocaldococcus jannaschii is specific for the hydrolysis and synthesis of S-inosyl-L-homocysteine, not S-adenosyl-L-homocysteine. No enzymatic activity is detected with S-adenosyl-L-homocysteine as the substrate in either the synthesis or hydrolysis direction. Enzyme SIHH shows a slight preference for L-homocysteine in the synthesis direction. It retains 94% activity when assayed with the same concentration of DL-homocysteine | Methanocaldococcus jannaschii NBRC 100440 | ? | - |
? | |
3.13.2.B1 | additional information | the annotated S-adenosyl-L-homocysteine hydrolase in Methanocaldococcus jannaschii is specific for the hydrolysis and synthesis of S-inosyl-L-homocysteine, not S-adenosyl-L-homocysteine. No enzymatic activity is detected with S-adenosyl-L-homocysteine as the substrate in either the synthesis or hydrolysis direction. Enzyme SIHH shows a slight preference for L-homocysteine in the synthesis direction. It retains 94% activity when assayed with the same concentration of DL-homocysteine | Methanocaldococcus jannaschii DSM 2661 | ? | - |
? | |
3.13.2.B1 | additional information | the annotated S-adenosyl-L-homocysteine hydrolase in Methanocaldococcus jannaschii is specific for the hydrolysis and synthesis of S-inosyl-L-homocysteine, not S-adenosyl-L-homocysteine. No enzymatic activity is detected with S-adenosyl-L-homocysteine as the substrate in either the synthesis or hydrolysis direction. Enzyme SIHH shows a slight preference for L-homocysteine in the synthesis direction. It retains 94% activity when assayed with the same concentration of DL-homocysteine | Methanocaldococcus jannaschii ATCC 43067 | ? | - |
? | |
3.13.2.B1 | additional information | the annotated S-adenosyl-L-homocysteine hydrolase in Methanocaldococcus jannaschii is specific for the hydrolysis and synthesis of S-inosyl-L-homocysteine, not S-adenosyl-L-homocysteine. No enzymatic activity is detected with S-adenosyl-L-homocysteine as the substrate in either the synthesis or hydrolysis direction. Enzyme SIHH shows a slight preference for L-homocysteine in the synthesis direction. It retains 94% activity when assayed with the same concentration of DL-homocysteine | Methanocaldococcus jannaschii JAL-1 | ? | - |
? | |
3.13.2.B1 | additional information | the annotated S-adenosyl-L-homocysteine hydrolase in Methanocaldococcus jannaschii is specific for the hydrolysis and synthesis of S-inosyl-L-homocysteine, not S-adenosyl-L-homocysteine. No enzymatic activity is detected with S-adenosyl-L-homocysteine as the substrate in either the synthesis or hydrolysis direction. Enzyme SIHH shows a slight preference for L-homocysteine in the synthesis direction. It retains 94% activity when assayed with the same concentration of DL-homocysteine | Methanocaldococcus jannaschii JCM 10045 | ? | - |
? | |
3.13.2.B1 | S-inosyl-L-homocysteine + H2O | - |
Methanocaldococcus jannaschii | L-homocysteine + inosine | - |
r | |
3.13.2.B1 | S-inosyl-L-homocysteine + H2O | - |
Methanocaldococcus jannaschii NBRC 100440 | L-homocysteine + inosine | - |
r | |
3.13.2.B1 | S-inosyl-L-homocysteine + H2O | - |
Methanocaldococcus jannaschii DSM 2661 | L-homocysteine + inosine | - |
r | |
3.13.2.B1 | S-inosyl-L-homocysteine + H2O | - |
Methanocaldococcus jannaschii ATCC 43067 | L-homocysteine + inosine | - |
r | |
3.13.2.B1 | S-inosyl-L-homocysteine + H2O | - |
Methanocaldococcus jannaschii JAL-1 | L-homocysteine + inosine | - |
r | |
3.13.2.B1 | S-inosyl-L-homocysteine + H2O | - |
Methanocaldococcus jannaschii JCM 10045 | L-homocysteine + inosine | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.13.1.9 | dimer | 2 * 49000, SDS-PAGE, the ratio of the tetramer form to the dimer form is 4:1 | Methanocaldococcus jannaschii |
3.13.1.9 | tetramer | 4 * 49000, SDS-PAGE, the ratio of the tetramer form to the dimer form is 4:1 | Methanocaldococcus jannaschii |
3.13.2.B1 | homotetramer | 4 * 49000, recombinant enzyme, SDS-PAGE, 4 * 46000, about, sequence calculation | Methanocaldococcus jannaschii |
3.13.2.B1 | More | the ratio of the tetramer form to the dimer form of SIHH is 4:1 based on their 280-nm absorbance. Peptide mapping with mass spectrometric analysis | Methanocaldococcus jannaschii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.13.1.9 | MJ1388 | - |
Methanocaldococcus jannaschii |
3.13.1.9 | SIHH | - |
Methanocaldococcus jannaschii |
3.13.2.B1 | MJ1388 | - |
Methanocaldococcus jannaschii |
3.13.2.B1 | SIHH | - |
Methanocaldococcus jannaschii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.13.1.9 | 70 | - |
- |
Methanocaldococcus jannaschii |
3.13.2.B1 | 70 | - |
- |
Methanocaldococcus jannaschii |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.13.1.9 | 80 | - |
the enzyme remains soluble up to 80°C | Methanocaldococcus jannaschii |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.13.1.9 | 0.42 | - |
S-Inosyl-L-homocysteine | pH 7.0, 70°C | Methanocaldococcus jannaschii | |
3.13.1.9 | 0.83 | - |
Inosine | pH 7.0, 70°C | Methanocaldococcus jannaschii | |
3.13.1.9 | 0.83 | - |
L-homocysteine | pH 7.0, 70°C | Methanocaldococcus jannaschii | |
3.13.2.B1 | 0.42 | - |
S-Inosyl-L-homocysteine | pH 7.5, 70°C, recombinant enzyme | Methanocaldococcus jannaschii | |
3.13.2.B1 | 0.83 | - |
Inosine | pH 7.5, 70°C, recombinant enzyme | Methanocaldococcus jannaschii | |
3.13.2.B1 | 0.83 | - |
L-homocysteine | pH 7.5, 70°C, recombinant enzyme | Methanocaldococcus jannaschii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.13.1.9 | 7 | 7.5 | the enzyme shows maximal activity at pH 7.0 to 7.5 and pH 9.6 | Methanocaldococcus jannaschii |
3.13.1.9 | 9.6 | - |
the enzyme shows maximal activity at pH 7.0 to 7.5 and pH 9.6 | Methanocaldococcus jannaschii |
3.13.2.B1 | 7 | 7.5 | optimum 1 | Methanocaldococcus jannaschii |
3.13.2.B1 | 9.6 | - |
optimum 2 | Methanocaldococcus jannaschii |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.13.1.9 | 7 | 11 | no measurable activity at either pH 6.5 or pH 11 and shows maximal activity at pH 7.0 to 7.5 and pH 9.6. pH 11: about 70% of maximal activity | Methanocaldococcus jannaschii |
3.13.2.B1 | 7 | 10 | enzyme SIHH has no measurable activity at either pH 6.5 or pH 11.0 and shows maximal activity at pH 7.0 to 7.5 and pH 9.6 | Methanocaldococcus jannaschii |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
3.13.1.9 | NAD+ | the enzyme binds an NAD+ cofactor. Apo-enzyme has no activity without the addition of NAD+ | Methanocaldococcus jannaschii | |
3.13.2.B1 | NAD+ | enzyme-bound, absolutely required for activity, addition of excess NAD+ (0.2 mM) increases enzymatic activity by about 2fold | Methanocaldococcus jannaschii |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.13.1.9 | 2 | - |
Inosine | pH 7.0, 70°C | Methanocaldococcus jannaschii |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.13.1.9 | physiological function | the enzyme participates in an alternative pathway for the regeneration of S-adenosyl-L-methionine from S-adenosyl-L-homocysteine that involves the deamination of the latter to S-inosyl-L-homocysteine | Methanocaldococcus jannaschii |
3.13.2.B1 | evolution | the S-inosyl-L-homocysteine hydrolase, which is specific for S-inosyl-L-homocysteine, shares with S-adenosyl-L-homocysteine hydrolase more than 45% sequence identity in Methanocaldococcus jannaschii | Methanocaldococcus jannaschii |
3.13.2.B1 | metabolism | canonical versus methanogenic (involving the enzyme) pathways for the recycling of S-adenosyl-L-homocysteine to S-adenosyl-L-methionine, overview | Methanocaldococcus jannaschii |
3.13.2.B1 | physiological function | enzyme S-inosyl-L-homocysteine hydrolase (SIHH) demonstrates a modified pathway in the methanogen for the regeneration of SAM from S-adenosyl-L-homocysteine that uses the deamination of S-adenosyl-L-homocysteine to form S-inosyl-L-homocysteine | Methanocaldococcus jannaschii |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.13.1.9 | 1.3 | - |
Inosine | pH 7.0, 70°C | Methanocaldococcus jannaschii | |
3.13.1.9 | 1.9 | - |
S-Inosyl-L-homocysteine | pH 7.0, 70°C | Methanocaldococcus jannaschii | |
3.13.1.9 | 152 | - |
L-homocysteine | pH 7.0, 70°C | Methanocaldococcus jannaschii | |
3.13.2.B1 | 1.3 | - |
Inosine | pH 7.5, 70°C, recombinant enzyme | Methanocaldococcus jannaschii | |
3.13.2.B1 | 1.9 | - |
S-Inosyl-L-homocysteine | pH 7.5, 70°C, recombinant enzyme | Methanocaldococcus jannaschii | |
3.13.2.B1 | 153.7 | - |
L-homocysteine | pH 7.5, 70°C, recombinant enzyme | Methanocaldococcus jannaschii |