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Literature summary extracted from

  • Miller, D.; Xu, H.; White, R.H.
    S-inosyl-L-homocysteine hydrolase, a novel enzyme involved in S-adenosyl-L-methionine recycling (2015), J. Bacteriol., 197, 2284-2291 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.13.2.B1 NAD+ addition of excess NAD+ (0.2 mM) increases enzymatic activity by about 2fold Methanocaldococcus jannaschii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.13.1.9 expression in Escherichia coli Methanocaldococcus jannaschii
3.13.2.B1 gene MJ1388, sequence comparisons and phylogenetic analysis, recombinant expression of the enzyme in Escherichia coli strain BL21-CodonPlus(DE3)-RIL Methanocaldococcus jannaschii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.13.1.9 Inosine above 2 mM Methanocaldococcus jannaschii
3.13.2.B1 additional information the D-isomer present in the DL-homocysteine is not a strong inhibitor of SIHH Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.13.1.9 0.0054
-
L-homocysteine pH 7.0, 70°C Methanocaldococcus jannaschii
3.13.1.9 0.22
-
S-Inosyl-L-homocysteine pH 7.0, 70°C Methanocaldococcus jannaschii
3.13.1.9 0.64
-
Inosine pH 7.0, 70°C Methanocaldococcus jannaschii
3.13.2.B1 0.0054
-
L-homocysteine pH 7.5, 70°C, recombinant enzyme Methanocaldococcus jannaschii
3.13.2.B1 0.22
-
S-Inosyl-L-homocysteine pH 7.5, 70°C, recombinant enzyme Methanocaldococcus jannaschii
3.13.2.B1 0.64
-
Inosine pH 7.5, 70°C, recombinant enzyme Methanocaldococcus jannaschii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.13.2.B1 cytoplasm
-
Methanocaldococcus jannaschii 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.13.1.9 49000
-
SDS-PAGE Methanocaldococcus jannaschii
3.13.2.B1 200000
-
about, gel filtration Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.13.1.9 S-inosyl-L-homocysteine + H2O Methanocaldococcus jannaschii the enzyme participates in an alternative pathway for the regeneration of S-adenosyl-L-methionine from S-adenosyl-L-homocysteine that involves the deamination of the latter to S-inosyl-L-homocysteine inosine + L-homocysteine
-
?
3.13.1.9 S-inosyl-L-homocysteine + H2O Methanocaldococcus jannaschii NBRC 100440 the enzyme participates in an alternative pathway for the regeneration of S-adenosyl-L-methionine from S-adenosyl-L-homocysteine that involves the deamination of the latter to S-inosyl-L-homocysteine inosine + L-homocysteine
-
?
3.13.1.9 S-inosyl-L-homocysteine + H2O Methanocaldococcus jannaschii DSM 2661 the enzyme participates in an alternative pathway for the regeneration of S-adenosyl-L-methionine from S-adenosyl-L-homocysteine that involves the deamination of the latter to S-inosyl-L-homocysteine inosine + L-homocysteine
-
?
3.13.1.9 S-inosyl-L-homocysteine + H2O Methanocaldococcus jannaschii ATCC 43067 the enzyme participates in an alternative pathway for the regeneration of S-adenosyl-L-methionine from S-adenosyl-L-homocysteine that involves the deamination of the latter to S-inosyl-L-homocysteine inosine + L-homocysteine
-
?
3.13.1.9 S-inosyl-L-homocysteine + H2O Methanocaldococcus jannaschii JAL-1 the enzyme participates in an alternative pathway for the regeneration of S-adenosyl-L-methionine from S-adenosyl-L-homocysteine that involves the deamination of the latter to S-inosyl-L-homocysteine inosine + L-homocysteine
-
?
3.13.1.9 S-inosyl-L-homocysteine + H2O Methanocaldococcus jannaschii JCM 10045 the enzyme participates in an alternative pathway for the regeneration of S-adenosyl-L-methionine from S-adenosyl-L-homocysteine that involves the deamination of the latter to S-inosyl-L-homocysteine inosine + L-homocysteine
-
?
3.13.2.B1 S-inosyl-L-homocysteine + H2O Methanocaldococcus jannaschii
-
L-homocysteine + inosine
-
r
3.13.2.B1 S-inosyl-L-homocysteine + H2O Methanocaldococcus jannaschii NBRC 100440
-
L-homocysteine + inosine
-
r
3.13.2.B1 S-inosyl-L-homocysteine + H2O Methanocaldococcus jannaschii DSM 2661
-
L-homocysteine + inosine
-
r
3.13.2.B1 S-inosyl-L-homocysteine + H2O Methanocaldococcus jannaschii ATCC 43067
-
L-homocysteine + inosine
-
r
3.13.2.B1 S-inosyl-L-homocysteine + H2O Methanocaldococcus jannaschii JAL-1
-
L-homocysteine + inosine
-
r
3.13.2.B1 S-inosyl-L-homocysteine + H2O Methanocaldococcus jannaschii JCM 10045
-
L-homocysteine + inosine
-
r

Organism

EC Number Organism UniProt Comment Textmining
3.13.1.9 Methanocaldococcus jannaschii Q58783
-
-
3.13.1.9 Methanocaldococcus jannaschii ATCC 43067 Q58783
-
-
3.13.2.B1 Methanocaldococcus jannaschii Q58783
-
-
3.13.2.B1 Methanocaldococcus jannaschii ATCC 43067 Q58783
-
-
3.13.2.B1 Methanocaldococcus jannaschii DSM 2661 Q58783
-
-
3.13.2.B1 Methanocaldococcus jannaschii JAL-1 Q58783
-
-
3.13.2.B1 Methanocaldococcus jannaschii JCM 10045 Q58783
-
-
3.13.2.B1 Methanocaldococcus jannaschii NBRC 100440 Q58783
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.13.1.9
-
Methanocaldococcus jannaschii
3.13.2.B1 recombinant enzyme from Escherichia coli strain BL21-CodonPlus(DE3)-RIL by heat treatment at 80°C for 10 min, and anion exchange chromatography Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.13.1.9 inosine + L-homocysteine the enzyme shows a slight preference for L-homocysteine in the synthesis direction Methanocaldococcus jannaschii S-inosyl-L-homocysteine + H2O
-
r
3.13.1.9 inosine + L-homocysteine the enzyme shows a slight preference for L-homocysteine in the synthesis direction Methanocaldococcus jannaschii NBRC 100440 S-inosyl-L-homocysteine + H2O
-
r
3.13.1.9 inosine + L-homocysteine the enzyme shows a slight preference for L-homocysteine in the synthesis direction Methanocaldococcus jannaschii DSM 2661 S-inosyl-L-homocysteine + H2O
-
r
3.13.1.9 inosine + L-homocysteine the enzyme shows a slight preference for L-homocysteine in the synthesis direction Methanocaldococcus jannaschii ATCC 43067 S-inosyl-L-homocysteine + H2O
-
r
3.13.1.9 inosine + L-homocysteine the enzyme shows a slight preference for L-homocysteine in the synthesis direction Methanocaldococcus jannaschii JAL-1 S-inosyl-L-homocysteine + H2O
-
r
3.13.1.9 inosine + L-homocysteine the enzyme shows a slight preference for L-homocysteine in the synthesis direction Methanocaldococcus jannaschii JCM 10045 S-inosyl-L-homocysteine + H2O
-
r
3.13.1.9 S-inosyl-L-homocysteine + H2O the enzyme participates in an alternative pathway for the regeneration of S-adenosyl-L-methionine from S-adenosyl-L-homocysteine that involves the deamination of the latter to S-inosyl-L-homocysteine Methanocaldococcus jannaschii inosine + L-homocysteine
-
?
3.13.1.9 S-inosyl-L-homocysteine + H2O the enzyme is specific for the hydrolysis and synthesis of S-inosyl-L-homocysteine, not S-adenosyl-L-homocysteine Methanocaldococcus jannaschii inosine + L-homocysteine
-
r
3.13.1.9 S-inosyl-L-homocysteine + H2O the enzyme participates in an alternative pathway for the regeneration of S-adenosyl-L-methionine from S-adenosyl-L-homocysteine that involves the deamination of the latter to S-inosyl-L-homocysteine Methanocaldococcus jannaschii NBRC 100440 inosine + L-homocysteine
-
?
3.13.1.9 S-inosyl-L-homocysteine + H2O the enzyme is specific for the hydrolysis and synthesis of S-inosyl-L-homocysteine, not S-adenosyl-L-homocysteine Methanocaldococcus jannaschii NBRC 100440 inosine + L-homocysteine
-
r
3.13.1.9 S-inosyl-L-homocysteine + H2O the enzyme participates in an alternative pathway for the regeneration of S-adenosyl-L-methionine from S-adenosyl-L-homocysteine that involves the deamination of the latter to S-inosyl-L-homocysteine Methanocaldococcus jannaschii DSM 2661 inosine + L-homocysteine
-
?
3.13.1.9 S-inosyl-L-homocysteine + H2O the enzyme is specific for the hydrolysis and synthesis of S-inosyl-L-homocysteine, not S-adenosyl-L-homocysteine Methanocaldococcus jannaschii DSM 2661 inosine + L-homocysteine
-
r
3.13.1.9 S-inosyl-L-homocysteine + H2O the enzyme participates in an alternative pathway for the regeneration of S-adenosyl-L-methionine from S-adenosyl-L-homocysteine that involves the deamination of the latter to S-inosyl-L-homocysteine Methanocaldococcus jannaschii ATCC 43067 inosine + L-homocysteine
-
?
3.13.1.9 S-inosyl-L-homocysteine + H2O the enzyme is specific for the hydrolysis and synthesis of S-inosyl-L-homocysteine, not S-adenosyl-L-homocysteine Methanocaldococcus jannaschii ATCC 43067 inosine + L-homocysteine
-
r
3.13.1.9 S-inosyl-L-homocysteine + H2O the enzyme participates in an alternative pathway for the regeneration of S-adenosyl-L-methionine from S-adenosyl-L-homocysteine that involves the deamination of the latter to S-inosyl-L-homocysteine Methanocaldococcus jannaschii JAL-1 inosine + L-homocysteine
-
?
3.13.1.9 S-inosyl-L-homocysteine + H2O the enzyme is specific for the hydrolysis and synthesis of S-inosyl-L-homocysteine, not S-adenosyl-L-homocysteine Methanocaldococcus jannaschii JAL-1 inosine + L-homocysteine
-
r
3.13.1.9 S-inosyl-L-homocysteine + H2O the enzyme participates in an alternative pathway for the regeneration of S-adenosyl-L-methionine from S-adenosyl-L-homocysteine that involves the deamination of the latter to S-inosyl-L-homocysteine Methanocaldococcus jannaschii JCM 10045 inosine + L-homocysteine
-
?
3.13.1.9 S-inosyl-L-homocysteine + H2O the enzyme is specific for the hydrolysis and synthesis of S-inosyl-L-homocysteine, not S-adenosyl-L-homocysteine Methanocaldococcus jannaschii JCM 10045 inosine + L-homocysteine
-
r
3.13.2.B1 L-homocysteine + inosine
-
Methanocaldococcus jannaschii S-inosyl-L-homocysteine + H2O
-
r
3.13.2.B1 additional information the annotated S-adenosyl-L-homocysteine hydrolase in Methanocaldococcus jannaschii is specific for the hydrolysis and synthesis of S-inosyl-L-homocysteine, not S-adenosyl-L-homocysteine. No enzymatic activity is detected with S-adenosyl-L-homocysteine as the substrate in either the synthesis or hydrolysis direction. Enzyme SIHH shows a slight preference for L-homocysteine in the synthesis direction. It retains 94% activity when assayed with the same concentration of DL-homocysteine Methanocaldococcus jannaschii ?
-
?
3.13.2.B1 additional information the annotated S-adenosyl-L-homocysteine hydrolase in Methanocaldococcus jannaschii is specific for the hydrolysis and synthesis of S-inosyl-L-homocysteine, not S-adenosyl-L-homocysteine. No enzymatic activity is detected with S-adenosyl-L-homocysteine as the substrate in either the synthesis or hydrolysis direction. Enzyme SIHH shows a slight preference for L-homocysteine in the synthesis direction. It retains 94% activity when assayed with the same concentration of DL-homocysteine Methanocaldococcus jannaschii NBRC 100440 ?
-
?
3.13.2.B1 additional information the annotated S-adenosyl-L-homocysteine hydrolase in Methanocaldococcus jannaschii is specific for the hydrolysis and synthesis of S-inosyl-L-homocysteine, not S-adenosyl-L-homocysteine. No enzymatic activity is detected with S-adenosyl-L-homocysteine as the substrate in either the synthesis or hydrolysis direction. Enzyme SIHH shows a slight preference for L-homocysteine in the synthesis direction. It retains 94% activity when assayed with the same concentration of DL-homocysteine Methanocaldococcus jannaschii DSM 2661 ?
-
?
3.13.2.B1 additional information the annotated S-adenosyl-L-homocysteine hydrolase in Methanocaldococcus jannaschii is specific for the hydrolysis and synthesis of S-inosyl-L-homocysteine, not S-adenosyl-L-homocysteine. No enzymatic activity is detected with S-adenosyl-L-homocysteine as the substrate in either the synthesis or hydrolysis direction. Enzyme SIHH shows a slight preference for L-homocysteine in the synthesis direction. It retains 94% activity when assayed with the same concentration of DL-homocysteine Methanocaldococcus jannaschii ATCC 43067 ?
-
?
3.13.2.B1 additional information the annotated S-adenosyl-L-homocysteine hydrolase in Methanocaldococcus jannaschii is specific for the hydrolysis and synthesis of S-inosyl-L-homocysteine, not S-adenosyl-L-homocysteine. No enzymatic activity is detected with S-adenosyl-L-homocysteine as the substrate in either the synthesis or hydrolysis direction. Enzyme SIHH shows a slight preference for L-homocysteine in the synthesis direction. It retains 94% activity when assayed with the same concentration of DL-homocysteine Methanocaldococcus jannaschii JAL-1 ?
-
?
3.13.2.B1 additional information the annotated S-adenosyl-L-homocysteine hydrolase in Methanocaldococcus jannaschii is specific for the hydrolysis and synthesis of S-inosyl-L-homocysteine, not S-adenosyl-L-homocysteine. No enzymatic activity is detected with S-adenosyl-L-homocysteine as the substrate in either the synthesis or hydrolysis direction. Enzyme SIHH shows a slight preference for L-homocysteine in the synthesis direction. It retains 94% activity when assayed with the same concentration of DL-homocysteine Methanocaldococcus jannaschii JCM 10045 ?
-
?
3.13.2.B1 S-inosyl-L-homocysteine + H2O
-
Methanocaldococcus jannaschii L-homocysteine + inosine
-
r
3.13.2.B1 S-inosyl-L-homocysteine + H2O
-
Methanocaldococcus jannaschii NBRC 100440 L-homocysteine + inosine
-
r
3.13.2.B1 S-inosyl-L-homocysteine + H2O
-
Methanocaldococcus jannaschii DSM 2661 L-homocysteine + inosine
-
r
3.13.2.B1 S-inosyl-L-homocysteine + H2O
-
Methanocaldococcus jannaschii ATCC 43067 L-homocysteine + inosine
-
r
3.13.2.B1 S-inosyl-L-homocysteine + H2O
-
Methanocaldococcus jannaschii JAL-1 L-homocysteine + inosine
-
r
3.13.2.B1 S-inosyl-L-homocysteine + H2O
-
Methanocaldococcus jannaschii JCM 10045 L-homocysteine + inosine
-
r

Subunits

EC Number Subunits Comment Organism
3.13.1.9 dimer 2 * 49000, SDS-PAGE, the ratio of the tetramer form to the dimer form is 4:1 Methanocaldococcus jannaschii
3.13.1.9 tetramer 4 * 49000, SDS-PAGE, the ratio of the tetramer form to the dimer form is 4:1 Methanocaldococcus jannaschii
3.13.2.B1 homotetramer 4 * 49000, recombinant enzyme, SDS-PAGE, 4 * 46000, about, sequence calculation Methanocaldococcus jannaschii
3.13.2.B1 More the ratio of the tetramer form to the dimer form of SIHH is 4:1 based on their 280-nm absorbance. Peptide mapping with mass spectrometric analysis Methanocaldococcus jannaschii

Synonyms

EC Number Synonyms Comment Organism
3.13.1.9 MJ1388
-
Methanocaldococcus jannaschii
3.13.1.9 SIHH
-
Methanocaldococcus jannaschii
3.13.2.B1 MJ1388
-
Methanocaldococcus jannaschii
3.13.2.B1 SIHH
-
Methanocaldococcus jannaschii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.13.1.9 70
-
-
Methanocaldococcus jannaschii
3.13.2.B1 70
-
-
Methanocaldococcus jannaschii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.13.1.9 80
-
the enzyme remains soluble up to 80°C Methanocaldococcus jannaschii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.13.1.9 0.42
-
S-Inosyl-L-homocysteine pH 7.0, 70°C Methanocaldococcus jannaschii
3.13.1.9 0.83
-
Inosine pH 7.0, 70°C Methanocaldococcus jannaschii
3.13.1.9 0.83
-
L-homocysteine pH 7.0, 70°C Methanocaldococcus jannaschii
3.13.2.B1 0.42
-
S-Inosyl-L-homocysteine pH 7.5, 70°C, recombinant enzyme Methanocaldococcus jannaschii
3.13.2.B1 0.83
-
Inosine pH 7.5, 70°C, recombinant enzyme Methanocaldococcus jannaschii
3.13.2.B1 0.83
-
L-homocysteine pH 7.5, 70°C, recombinant enzyme Methanocaldococcus jannaschii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.13.1.9 7 7.5 the enzyme shows maximal activity at pH 7.0 to 7.5 and pH 9.6 Methanocaldococcus jannaschii
3.13.1.9 9.6
-
the enzyme shows maximal activity at pH 7.0 to 7.5 and pH 9.6 Methanocaldococcus jannaschii
3.13.2.B1 7 7.5 optimum 1 Methanocaldococcus jannaschii
3.13.2.B1 9.6
-
optimum 2 Methanocaldococcus jannaschii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.13.1.9 7 11 no measurable activity at either pH 6.5 or pH 11 and shows maximal activity at pH 7.0 to 7.5 and pH 9.6. pH 11: about 70% of maximal activity Methanocaldococcus jannaschii
3.13.2.B1 7 10 enzyme SIHH has no measurable activity at either pH 6.5 or pH 11.0 and shows maximal activity at pH 7.0 to 7.5 and pH 9.6 Methanocaldococcus jannaschii

Cofactor

EC Number Cofactor Comment Organism Structure
3.13.1.9 NAD+ the enzyme binds an NAD+ cofactor. Apo-enzyme has no activity without the addition of NAD+ Methanocaldococcus jannaschii
3.13.2.B1 NAD+ enzyme-bound, absolutely required for activity, addition of excess NAD+ (0.2 mM) increases enzymatic activity by about 2fold Methanocaldococcus jannaschii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.13.1.9 2
-
Inosine pH 7.0, 70°C Methanocaldococcus jannaschii

General Information

EC Number General Information Comment Organism
3.13.1.9 physiological function the enzyme participates in an alternative pathway for the regeneration of S-adenosyl-L-methionine from S-adenosyl-L-homocysteine that involves the deamination of the latter to S-inosyl-L-homocysteine Methanocaldococcus jannaschii
3.13.2.B1 evolution the S-inosyl-L-homocysteine hydrolase, which is specific for S-inosyl-L-homocysteine, shares with S-adenosyl-L-homocysteine hydrolase more than 45% sequence identity in Methanocaldococcus jannaschii Methanocaldococcus jannaschii
3.13.2.B1 metabolism canonical versus methanogenic (involving the enzyme) pathways for the recycling of S-adenosyl-L-homocysteine to S-adenosyl-L-methionine, overview Methanocaldococcus jannaschii
3.13.2.B1 physiological function enzyme S-inosyl-L-homocysteine hydrolase (SIHH) demonstrates a modified pathway in the methanogen for the regeneration of SAM from S-adenosyl-L-homocysteine that uses the deamination of S-adenosyl-L-homocysteine to form S-inosyl-L-homocysteine Methanocaldococcus jannaschii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.13.1.9 1.3
-
Inosine pH 7.0, 70°C Methanocaldococcus jannaschii
3.13.1.9 1.9
-
S-Inosyl-L-homocysteine pH 7.0, 70°C Methanocaldococcus jannaschii
3.13.1.9 152
-
L-homocysteine pH 7.0, 70°C Methanocaldococcus jannaschii
3.13.2.B1 1.3
-
Inosine pH 7.5, 70°C, recombinant enzyme Methanocaldococcus jannaschii
3.13.2.B1 1.9
-
S-Inosyl-L-homocysteine pH 7.5, 70°C, recombinant enzyme Methanocaldococcus jannaschii
3.13.2.B1 153.7
-
L-homocysteine pH 7.5, 70°C, recombinant enzyme Methanocaldococcus jannaschii