Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Avinash, V.S.; Ramasamy, S.; Suresh, C.G.; Pundle, A.
    Penicillin V acylase from Pectobacterium atrosepticum exhibits high specific activity and unique kinetics (2015), Int. J. Biol. Macromol., 79, 1-7 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.11 2-butanone 5% (v/v), 30 min at room temperature, 1.74fold activation Pectobacterium atrosepticum
3.5.1.11 2-mercaptoethanol 1 mM, 1.19fold activation Pectobacterium atrosepticum
3.5.1.11 acetonitrile 5% (v/v), 30 min at room temperature, 1.65fold activation Pectobacterium atrosepticum
3.5.1.11 CTAB 0.1%, 2.5fold activation Pectobacterium atrosepticum
3.5.1.11 dithiothreitol 10 mM, 1.23fold activation Pectobacterium atrosepticum
3.5.1.11 ethyl acetate 5% (v/v), 30 min at room temperature, 1.47fold activation Pectobacterium atrosepticum
3.5.1.11 hexane 5% (v/v), 30 min at room temperature, 1.13fold activation Pectobacterium atrosepticum
3.5.1.11 Isopropanol 5% (v/v), 30 min at room temperature, 1.95fold activation Pectobacterium atrosepticum
3.5.1.11 Triton X-100 0.1%, 1.8fold activation Pectobacterium atrosepticum
3.5.1.11 Tween 20 0.1%, 1.75fold activation Pectobacterium atrosepticum

Application

EC Number Application Comment Organism
3.5.1.11 pharmacology both the recombinant protein yield and the specific activity of the enzyme are very high, auguring well for application in the pharmaceutical industry for production of the pharmaceutical intermediates 6-amino penicillanic acid. Penicillin acylases find use in the pharmaceutical industry for the production of semi-synthetic antibiotics Pectobacterium atrosepticum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.11 expressed in Escherichia coli, attaining a very high yield (250 mg/l) and a comparatively high specific activity (430 IU/mg) Pectobacterium atrosepticum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.11 SDS 0.1%, 90% loss of activity Pectobacterium atrosepticum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.11 154000
-
gel filtration Pectobacterium atrosepticum

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.11 Pectobacterium atrosepticum Q6D291
-
-
3.5.1.11 Pectobacterium atrosepticum ATCC BAA-672 Q6D291
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.11
-
Pectobacterium atrosepticum

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.5.1.11 the enzyme starts progressively losing activity after 1.5 M guanidine hydrochloride, and is completelly inactivated at concentrations over 2 M. Dilution of guanidine hydrochloride leads to revival of the original enzyme activity within 1 h, indicating that unfolding is completely reversible Pectobacterium atrosepticum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.11 2-nitro-5-(phenoxyacetamido)-benzoic acid + H2O
-
Pectobacterium atrosepticum 5-amino-2-nitrobenzoic acid + phenoxyacetate
-
?
3.5.1.11 2-nitro-5-(phenoxyacetamido)-benzoic acid + H2O
-
Pectobacterium atrosepticum ATCC BAA-672 5-amino-2-nitrobenzoic acid + phenoxyacetate
-
?
3.5.1.11 penicillin V + H2O
-
Pectobacterium atrosepticum 6-aminopenicillanic acid + phenoxyacetic acid
-
?
3.5.1.11 penicillin V + H2O
-
Pectobacterium atrosepticum ATCC BAA-672 6-aminopenicillanic acid + phenoxyacetic acid
-
?

Subunits

EC Number Subunits Comment Organism
3.5.1.11 tetramer 4 * 39191, matrix-associated laser desorption ionization-mass spectrometry Pectobacterium atrosepticum

Synonyms

EC Number Synonyms Comment Organism
3.5.1.11 Eca3205
-
Pectobacterium atrosepticum
3.5.1.11 penicillin V acylase
-
Pectobacterium atrosepticum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.11 45
-
-
Pectobacterium atrosepticum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.11 35 60 35°C: about 60% of maximal activity, 60°C: about 35% of maximal activity Pectobacterium atrosepticum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.11 30
-
15 min, the enzyme retains 87% of its original activity Pectobacterium atrosepticum
3.5.1.11 40
-
90 min, about 35% loss of activity Pectobacterium atrosepticum
3.5.1.11 50
-
90 min, about 50% loss of activity Pectobacterium atrosepticum
3.5.1.11 60
-
60 min, about 80% loss of activity Pectobacterium atrosepticum
3.5.1.11 70
-
30 min, about 80% loss of activity Pectobacterium atrosepticum
3.5.1.11 80
-
15 min, about 90% loss of activity Pectobacterium atrosepticum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.11 5
-
acetate buffer Pectobacterium atrosepticum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.11 4 6 pH 4.0: about 40% of maximal activity, pH 6.0: about 35% of maximal activity Pectobacterium atrosepticum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.11 3 7 the enzyme is functionally stable in the pH range 3-7 with than 80% retention in activity after 4 h. Significant reduction in activity at pH 9-11, with only 40% of the original activity retained. The enzyme is rapidly inactivated at pH 1-2 Pectobacterium atrosepticum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.5.1.11 Pectobacterium atrosepticum
-
-
8.4