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Literature summary extracted from

  • Sadeghi-Kaji, S.; Shareghi, B.; Saboury, A.A.; Farhadian, S.
    Spectroscopic and molecular docking studies on the interaction between spermidine and pancreatic elastase (2019), Int. J. Biol. Macromol., 131, 473-483 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.36 spermidine spermidine serves as a partial stabilizer and an activator for the enzyme Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.36 0.07761
-
 4-nitrophenyl acetate in the absence of spermidine, at pH 8.5 and 37°C Sus scrofa
3.4.21.36 0.08229
-
 4-nitrophenyl acetate in the presence of 0.2 mM spermidine, at pH 8.5 and 37°C Sus scrofa
3.4.21.36 0.09364
-
 4-nitrophenyl acetate in the presence of 0.3 mM spermidine, at pH 8.5 and 37°C Sus scrofa
3.4.21.36 0.11568
-
 4-nitrophenyl acetate in the presence of 0.5 mM spermidine, at pH 8.5 and 37°C Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.36 Sus scrofa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.36 pancreas
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.36 4-nitrophenyl acetate + H2O
-
 Sus scrofa 4-nitrophenol + acetate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.36 pancreatic elastase
-
 Sus scrofa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.36 60
-
 melting temperature Sus scrofa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.36 0.0058
-
 4-nitrophenyl acetate in the absence of spermidine, at pH 8.5 and 37°C Sus scrofa
3.4.21.36 0.007
-
 4-nitrophenyl acetate in the presence of 0.2 mM spermidine, at pH 8.5 and 37°C Sus scrofa
3.4.21.36 0.0092
-
 4-nitrophenyl acetate in the presence of 0.3 mM spermidine, at pH 8.5 and 37°C Sus scrofa
3.4.21.36 0.0125
-
 4-nitrophenyl acetate in the presence of 0.5 mM spermidine, at pH 8.5 and 37°C Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.36 8.5
-
-
 Sus scrofa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.21.36 0.075
-
 4-nitrophenyl acetate in the absence of spermidine, at pH 8.5 and 37°C Sus scrofa
3.4.21.36 0.085
-
 4-nitrophenyl acetate in the presence of 0.2 mM spermidine, at pH 8.5 and 37°C Sus scrofa
3.4.21.36 0.097
-
 4-nitrophenyl acetate in the presence of 0.3 mM spermidine, at pH 8.5 and 37°C Sus scrofa
3.4.21.36 0.107
-
 4-nitrophenyl acetate in the presence of 0.5 mM spermidine, at pH 8.5 and 37°C Sus scrofa