EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.1.124 | DJ-1 gene is subcloned from pLenti6-DJ-1-V5-WT plasmid into the pRetroX-Tight-Puro Advanced vector. The confirmed clone is transfected into HEK-293 cells | Homo sapiens |
4.2.1.130 | DJ-1 gene is subcloned from pLenti6-DJ-1-V5-WT plasmid into the pRetroX-Tight-Puro Advanced vector. The confirmed clone is transfected into HEK-293 cells | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.1.130 | L166P | the Km-value for methylglyoxal of the mutant enzyme is about 2fold higher compared to the wild-type enzyme | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.130 | 900 | - |
methylglyoxal | 30°C, pH not specified in the publication, wild-type enzyme | Homo sapiens | |
4.2.1.130 | 1900 | - |
methylglyoxal | 30°C, pH not specified in the publication, mutant enzyme L166P | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.130 | methylglyoxal + H2O | Homo sapiens | - |
(R)-lactate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.124 | Homo sapiens | Q99497 | cf. EC 4.2.1.130 | - |
4.2.1.130 | Homo sapiens | Q99497 | cf. EC 3.5.1.124 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.1.124 | - |
Homo sapiens |
4.2.1.130 | - |
Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.124 | N6-(1-hydroxy-2-oxopropyl)-[alpha-synuclein]-L-lysine + H2O | - |
Homo sapiens | [alpha-synuclein]-L-lysine + lactate | - |
? | |
4.2.1.130 | methylglyoxal + H2O | - |
Homo sapiens | (R)-lactate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.1.124 | DJ-1 | - |
Homo sapiens |
4.2.1.130 | DJ-1 | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.124 | 30 | - |
assay at | Homo sapiens |
4.2.1.130 | 30 | - |
assay at | Homo sapiens |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.5.1.124 | malfunction | reduced DJ-1 activity due to mutations or oxidative stress may lead to the accumulation of glycated alpha-synuclein and its aggregates | Homo sapiens |
3.5.1.124 | physiological function | the deglycase activity of DJ-1 mitigates alpha-synuclein glycation and aggregation in dopaminergic cells | Homo sapiens |