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Literature summary extracted from

  • Luo, Q.; Chen, D.; Boom, R.M.; Janssen, A.E.M.
    Revisiting the enzymatic kinetics of pepsin using isothermal titration calorimetry (2018), Food Chem., 268, 94-100 .
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.23.1 0.04
-
benzyloxycarbonyl-Phe-Phe-OEt at 43 mM ionic strength, at pH 5.5 and 37°C Sus scrofa
3.4.23.1 0.2
-
bovine serum albumin at 43 mM ionic strength, at pH 2.0 and 37°C Sus scrofa
3.4.23.1 0.2
-
benzyloxycarbonyl-Phe-Phe 3-(4-pyridyl)propyl ester at pH 3.0 and 37°C Sus scrofa
3.4.23.1 0.26
-
benzyloxycarbonyl-Phe-Phe-OEt at pH 1.0 and 37°C Sus scrofa
3.4.23.1 0.33
-
benzyloxycarbonyl-Phe-Phe-OEt at pH 2.0 and 37°C Sus scrofa
3.4.23.1 0.37
-
benzyloxycarbonyl-Phe-Phe-OEt at pH 1.5 and 37°C Sus scrofa
3.4.23.1 0.4
-
benzyloxycarbonyl-Gly-Phe-Phe 3-(4-pyridyl)propyl ester at pH 3.5 and 37°C Sus scrofa
3.4.23.1 0.4
-
benzyloxycarbonyl-Gly-Gly-Phe-Phe 3-(4-pyridyl)propyl ester at pH 3.5 and 37°C Sus scrofa
3.4.23.1 0.7
-
benzyloxycarbonyl-Phe-Phe 3-(4-pyridyl)propyl ester at pH 2.0 and 37°C Sus scrofa
3.4.23.1 0.8
-
benzyloxycarbonyl-Gly-Gly-Phe-Phe 3-(4-pyridyl)propyl ester at pH 2.0 and 37°C Sus scrofa
3.4.23.1 1.1
-
benzyloxycarbonyl-Gly-Phe-Phe 3-(4-pyridyl)propyl ester at pH 2.0 and 37°C Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.4.23.1 Sus scrofa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.23.1 gastric mucosa
-
Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.23.1 benzyloxycarbonyl-Gly-Gly-Phe-Phe 3-(4-pyridyl)propyl ester + H2O
-
Sus scrofa benzyloxycarbonyl-Gly-Gly-Phe + Phe 3-(4-pyridyl)propyl ester
-
?
3.4.23.1 benzyloxycarbonyl-Gly-Phe-Phe 3-(4-pyridyl)propyl ester + H2O
-
Sus scrofa benzyloxycarbonyl-Gly-Phe + Phe 3-(4-pyridyl)propyl ester
-
?
3.4.23.1 benzyloxycarbonyl-Phe-Phe 3-(4-pyridyl)propyl ester + H2O
-
Sus scrofa benzyloxycarbonyl-Phe + Phe 3-(4-pyridyl)propyl ester
-
?
3.4.23.1 benzyloxycarbonyl-Phe-Phe-OEt + H2O
-
Sus scrofa benzyloxycarbonyl-Phe + Phe-OEt
-
?
3.4.23.1 Bovine serum albumin + H2O
-
Sus scrofa ?
-
?
3.4.23.1 Z-GGFF-OP4P + H2O
-
Sus scrofa benzyloxycarbonyl-GGF + Phe 3-(4-pyridyl)propyl ester
-
?

Subunits

EC Number Subunits Comment Organism
3.4.23.1 ? x * 34600, calculated from amino acid sequence Sus scrofa

Synonyms

EC Number Synonyms Comment Organism
3.4.23.1 pepsin
-
Sus scrofa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.23.1 0.08
-
benzyloxycarbonyl-Phe-Phe-OEt at 43 mM ionic strength, at pH 5.5 and 37°C Sus scrofa
3.4.23.1 0.1
-
benzyloxycarbonyl-Phe-Phe-OEt at pH 1.0 and 37°C Sus scrofa
3.4.23.1 0.11
-
benzyloxycarbonyl-Phe-Phe-OEt at pH 2.0 and 37°C Sus scrofa
3.4.23.1 0.13
-
benzyloxycarbonyl-Phe-Phe-OEt at pH 1.5 and 37°C Sus scrofa
3.4.23.1 0.49
-
benzyloxycarbonyl-Phe-Phe 3-(4-pyridyl)propyl ester at pH 2.0 and 37°C Sus scrofa
3.4.23.1 0.74
-
benzyloxycarbonyl-Phe-Phe 3-(4-pyridyl)propyl ester at pH 3.0 and 37°C Sus scrofa
3.4.23.1 2.2
-
benzyloxycarbonyl-Gly-Phe-Phe 3-(4-pyridyl)propyl ester at pH 2.0 and 37°C Sus scrofa
3.4.23.1 3.1
-
benzyloxycarbonyl-Gly-Phe-Phe 3-(4-pyridyl)propyl ester at pH 3.5 and 37°C Sus scrofa
3.4.23.1 4.44
-
bovine serum albumin at 43 mM ionic strength, at pH 2.0 and 37°C Sus scrofa
3.4.23.1 56.5
-
benzyloxycarbonyl-Gly-Gly-Phe-Phe 3-(4-pyridyl)propyl ester at pH 2.0 and 37°C Sus scrofa
3.4.23.1 71.8
-
benzyloxycarbonyl-Gly-Gly-Phe-Phe 3-(4-pyridyl)propyl ester at pH 3.5 and 37°C Sus scrofa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.23.1 0.31
-
benzyloxycarbonyl-Phe-Phe-OEt at pH 2.0 and 37°C Sus scrofa
3.4.23.1 0.35
-
benzyloxycarbonyl-Phe-Phe-OEt at pH 1.5 and 37°C Sus scrofa
3.4.23.1 0.39
-
benzyloxycarbonyl-Phe-Phe-OEt at pH 1.0 and 37°C Sus scrofa
3.4.23.1 0.7
-
benzyloxycarbonyl-Phe-Phe 3-(4-pyridyl)propyl ester at pH 2.0 and 37°C Sus scrofa
3.4.23.1 2
-
benzyloxycarbonyl-Gly-Phe-Phe 3-(4-pyridyl)propyl ester at pH 2.0 and 37°C Sus scrofa
3.4.23.1 2.65
-
benzyloxycarbonyl-Phe-Phe-OEt at 43 mM ionic strength, at pH 5.5 and 37°C Sus scrofa
3.4.23.1 3.7
-
benzyloxycarbonyl-Phe-Phe 3-(4-pyridyl)propyl ester at pH 3.0 and 37°C Sus scrofa
3.4.23.1 7.8
-
benzyloxycarbonyl-Gly-Phe-Phe 3-(4-pyridyl)propyl ester at pH 3.5 and 37°C Sus scrofa
3.4.23.1 22.1
-
bovine serum albumin at 43 mM ionic strength, at pH 2.0 and 37°C Sus scrofa
3.4.23.1 70.6
-
benzyloxycarbonyl-Gly-Gly-Phe-Phe 3-(4-pyridyl)propyl ester at pH 2.0 and 37°C Sus scrofa
3.4.23.1 180
-
benzyloxycarbonyl-Gly-Gly-Phe-Phe 3-(4-pyridyl)propyl ester at pH 3.5 and 37°C Sus scrofa