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Literature summary extracted from

  • Curci, N.; Strazzulli, A.; De Lise, F.; Iacono, R.; Maurelli, L.; Dal Piaz, F.; Cobucci-Ponzano, B.; Moracci, M.
    Identification of a novel esterase from the thermophilic bacterium Geobacillus thermodenitrificans NG80-2 (2019), Extremophiles, 23, 407-419 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.108 2-mercaptoethanol about 160% activity at 10 mM Geobacillus thermodenitrificans
3.1.1.108 dithiothreitol about 140% activity at 1 mM Geobacillus thermodenitrificans
3.1.1.108 EDTA about 125% activity at 1 mM Geobacillus thermodenitrificans
3.1.1.108 n-hexane about 140% activity at 30% (v/v) Geobacillus thermodenitrificans
3.1.1.108 Triton X-100 about 150% activity at 10 mM Geobacillus thermodenitrificans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.108 expressed in Escherichia coli BL21 Star (DE3) One Shot cells Geobacillus thermodenitrificans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.108 acetone about 60% residual activity at 30% (v/v) Geobacillus thermodenitrificans
3.1.1.108 acetonitrile about 30% residual activity at 10% (v/v) Geobacillus thermodenitrificans
3.1.1.108 chloroform about 40% residual activity at 30% (v/v) Geobacillus thermodenitrificans
3.1.1.108 Cu2+ about 70% residual activity at 10 mM Geobacillus thermodenitrificans
3.1.1.108 dimethylformamide about 50% residual activity at 10% (v/v) Geobacillus thermodenitrificans
3.1.1.108 DMSO about 60% residual activity at 30% (v/v) Geobacillus thermodenitrificans
3.1.1.108 ethanol about 40% residual activity at 30% (v/v) Geobacillus thermodenitrificans
3.1.1.108 Fe2+ about 70% residual activity at 10 mM Geobacillus thermodenitrificans
3.1.1.108 K+ about 70% residual activity at 10 mM Geobacillus thermodenitrificans
3.1.1.108 methanol about 50% residual activity at 30% (v/v) Geobacillus thermodenitrificans
3.1.1.108 additional information EDTA has no inhibiting effect Geobacillus thermodenitrificans
3.1.1.108 n-butanol about 30% residual activity at 10% (v/v) Geobacillus thermodenitrificans
3.1.1.108 phenylmethylsulfonyl fluoride about 25% residual activity at 1 mM Geobacillus thermodenitrificans
3.1.1.108 propan-2-ol about 30% residual activity at 10% (v/v) Geobacillus thermodenitrificans
3.1.1.108 SDS about 60% residual activity at 10 mM Geobacillus thermodenitrificans
3.1.1.108 Zn2+ about 70% residual activity at 10 mM Geobacillus thermodenitrificans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.108 0.005
-
 4-nitrophenyl caprylate at pH 7.5 and 55°C Geobacillus thermodenitrificans
3.1.1.108 0.64
-
 4-nitrophenyl butyrate at pH 7.5 and 55°C Geobacillus thermodenitrificans
3.1.1.108 0.97
-
 4-nitrophenyl acetate at pH 7.5 and 55°C Geobacillus thermodenitrificans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.108 Mn2+ about 110% activity at 10 mM Geobacillus thermodenitrificans
3.1.1.108 additional information not influenced by Ca2+ and Mg2+ Geobacillus thermodenitrificans
3.1.1.108 Na+ about 110% activity at 10 mM Geobacillus thermodenitrificans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.108 26000
-
 gel filtration Geobacillus thermodenitrificans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.108 iron(III)-enterobactin + 3 H2O Geobacillus thermodenitrificans overall reaction iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine complex + 2 N-(2,3-dihydroxybenzoyl)-L-serine
-
 ?
3.1.1.108 iron(III)-enterobactin + 3 H2O Geobacillus thermodenitrificans NG80-2 overall reaction iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine complex + 2 N-(2,3-dihydroxybenzoyl)-L-serine
-
 ?
3.1.1.108 iron(III)-enterobactin + H2O Geobacillus thermodenitrificans
-
 iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine trimer complex + N-(2,3-dihydroxybenzoyl)-L-serine
-
 ?
3.1.1.108 iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine dimer complex + H2O Geobacillus thermodenitrificans
-
 iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine complex + N-(2,3-dihydroxybenzoyl)-L-serine
-
 ?
3.1.1.108 iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine trimer complex + H2O Geobacillus thermodenitrificans
-
 iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine dimer complex + N-(2,3-dihydroxybenzoyl)-L-serine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.108 Geobacillus thermodenitrificans A0A1W6VPA7
-
-
3.1.1.108 Geobacillus thermodenitrificans NG80-2 A0A1W6VPA7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.108 His Trap column chromatography, Ni-TED 1000 column chromatography, and Superdex 75 gel filtration Geobacillus thermodenitrificans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.108 4-nitrophenyl acetate + H2O best substrate Geobacillus thermodenitrificans 4-nitrophenol + acetate
-
 ?
3.1.1.108 4-nitrophenyl acetate + H2O best substrate Geobacillus thermodenitrificans NG80-2 4-nitrophenol + acetate
-
 ?
3.1.1.108 4-nitrophenyl butyrate + H2O
-
 Geobacillus thermodenitrificans 4-nitrophenol + butyrate
-
 ?
3.1.1.108 4-nitrophenyl butyrate + H2O
-
 Geobacillus thermodenitrificans NG80-2 4-nitrophenol + butyrate
-
 ?
3.1.1.108 4-nitrophenyl caprylate + H2O
-
 Geobacillus thermodenitrificans 4-nitrophenol + caprylate
-
 ?
3.1.1.108 4-nitrophenyl caprylate + H2O
-
 Geobacillus thermodenitrificans NG80-2 4-nitrophenol + caprylate
-
 ?
3.1.1.108 iron(III)-enterobactin + 3 H2O overall reaction Geobacillus thermodenitrificans iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine complex + 2 N-(2,3-dihydroxybenzoyl)-L-serine
-
 ?
3.1.1.108 iron(III)-enterobactin + 3 H2O overall reaction Geobacillus thermodenitrificans NG80-2 iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine complex + 2 N-(2,3-dihydroxybenzoyl)-L-serine
-
 ?
3.1.1.108 iron(III)-enterobactin + H2O
-
 Geobacillus thermodenitrificans iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine trimer complex + N-(2,3-dihydroxybenzoyl)-L-serine
-
 ?
3.1.1.108 iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine dimer complex + H2O
-
 Geobacillus thermodenitrificans iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine complex + N-(2,3-dihydroxybenzoyl)-L-serine
-
 ?
3.1.1.108 iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine trimer complex + H2O
-
 Geobacillus thermodenitrificans iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine dimer complex + N-(2,3-dihydroxybenzoyl)-L-serine
-
 ?
3.1.1.108 additional information no activity with 4-nitrophenyl laurate, bacillibactin and enterobactin Geobacillus thermodenitrificans ?
-
 ?
3.1.1.108 additional information no activity with 4-nitrophenyl laurate, bacillibactin and enterobactin Geobacillus thermodenitrificans NG80-2 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.108 monomer 1 * 30000, SDS-PAGE Geobacillus thermodenitrificans
3.1.1.108 monomer 1 * 25940, calculated from amino acid sequence Geobacillus thermodenitrificans

Synonyms

EC Number Synonyms Comment Organism
3.1.1.108 EstGtA3
-
 Geobacillus thermodenitrificans
3.1.1.108 GTNG_0744
-
 Geobacillus thermodenitrificans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.108 60
-
-
 Geobacillus thermodenitrificans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.108 55 65 the enzyme is 100% stable when incubated for 16 h at 55°C. About 20% residual activity is observed after 5 min at 65°C Geobacillus thermodenitrificans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.108 1.24
-
 4-nitrophenyl caprylate at pH 7.5 and 55°C Geobacillus thermodenitrificans
3.1.1.108 3.03
-
 4-nitrophenyl butyrate at pH 7.5 and 55°C Geobacillus thermodenitrificans
3.1.1.108 6.8
-
 4-nitrophenyl acetate at pH 7.5 and 55°C Geobacillus thermodenitrificans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.108 7 8.5 the enzyme is active in the pH range 7.0-8.5 Geobacillus thermodenitrificans

Cofactor

EC Number Cofactor Comment Organism Structure
3.1.1.108 additional information the enzyme does not require cofactors for its activity Geobacillus thermodenitrificans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.108 4.73
-
 4-nitrophenyl butyrate at pH 7.5 and 55°C Geobacillus thermodenitrificans
3.1.1.108 7.01
-
 4-nitrophenyl acetate at pH 7.5 and 55°C Geobacillus thermodenitrificans
3.1.1.108 248
-
 4-nitrophenyl caprylate at pH 7.5 and 55°C Geobacillus thermodenitrificans