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Literature summary extracted from

  • Mohamed, S.A.; Elshal, M.F.; Kumosani, T.A.; Aldahlawi, A.M.
    Purification and characterization of asparaginase from Phaseolus vulgaris seeds (2015), Evid. Based. Complement Alternat. Med., 2015, 309214 .
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.1 EDTA 10 mM, 62% residual activity Phaseolus vulgaris
3.5.1.1 Hg2+ 10 mM, 33% residual activity Phaseolus vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.1 6.72
-
 L-asparagine pH 8.0, 37°C Phaseolus vulgaris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.1 Ca2+ 10 mM, 110% of initial activity Phaseolus vulgaris
3.5.1.1 K+ 10 mM, 150% of initial activity Phaseolus vulgaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.1 79000
-
 gel filtration Phaseolus vulgaris

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Phaseolus vulgaris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.1
-
 Phaseolus vulgaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.1 seed
-
 Phaseolus vulgaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.1 846
-
 pH 8.0, 37°C Phaseolus vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 L-asparagine + H2O
-
 Phaseolus vulgaris L-aspartate + NH3
-
 ?
3.5.1.1 additional information poor substrates: D-asparagine, L-aspartic acid, and L-glutamic acid. No substrate: L-glutamine Phaseolus vulgaris ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.1 monomer 1 * 79000, SDS-PAGE Phaseolus vulgaris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.1 37
-
-
 Phaseolus vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.1 8
-
-
 Phaseolus vulgaris

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.1 6 9 more than 50% of maximum activity Phaseolus vulgaris