Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Galdino, A.C.M.; de Oliveira, M.P.; Ramalho, T.C.; de Castro, A.A.; Branquinha, M.H.; Santos, A.L.S.
    Anti-virulence strategy against the multidrug-resistant bacterial pathogen Pseudomonas aeruginosa pseudolysin (elastase B) as a potential druggable target (2019), Curr. Protein Pept. Sci., 20, 471-487 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.24.26 medicine the enzyme is a promising target for the development of new anti-virulence compounds Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.26 1,10-phenanthroline 1 mM, complete inhibition Pseudomonas aeruginosa
3.4.24.26 1-(biphenyl-4-ylmethyl)-3-hydroxy-2-methylpyridine-4(1H)-thione
-
Pseudomonas aeruginosa
3.4.24.26 5-fluorouracil
-
Pseudomonas aeruginosa
3.4.24.26 Baicalin
-
Pseudomonas aeruginosa
3.4.24.26 dehydroleucodine
-
Pseudomonas aeruginosa
3.4.24.26 EDTA
-
Pseudomonas aeruginosa
3.4.24.26 EGTA
-
Pseudomonas aeruginosa
3.4.24.26 eriodictyol
-
Pseudomonas aeruginosa
3.4.24.26 eugenol
-
Pseudomonas aeruginosa
3.4.24.26 HS-CH2-CO-Phe-Tyr-NH2 at 0.2 mM and 0.025 mM inhibits the degradation of the pseudolysin natural substrates nucleoside diphosphate kinase and IgG, respectively Pseudomonas aeruginosa
3.4.24.26 HSAc-Leu-Phe 0.1 mM, inhibits 97% of the degradation of azocasein and elastin substrates by pseudolysin Pseudomonas aeruginosa
3.4.24.26 HSAc-Phe-Leu 0.1 mM, inhibits 97% of the degradation of azocasein and elastin substrates by pseudolysin Pseudomonas aeruginosa
3.4.24.26 N-aryl mercaptoacetamide
-
Pseudomonas aeruginosa
3.4.24.26 N-mercaptoacetyl-Phe-Tyr-amide
-
Pseudomonas aeruginosa
3.4.24.26 naringenin
-
Pseudomonas aeruginosa
3.4.24.26 phosphoramidon powerful inhibition action on pseudolysin Pseudomonas aeruginosa
3.4.24.26 taxifolin
-
Pseudomonas aeruginosa
3.4.24.26 vitexin
-
Pseudomonas aeruginosa
3.4.24.26 zingerone
-
Pseudomonas aeruginosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.26 extracellular most abundant peptidase found in pseudomonal secretions, which contributes to the invasiveness of Pseudomonas aeruginosa due to its ability to cleave several extracellular matrix proteins and to disrupt the basolateral intercellular functions present in the host tissues Pseudomonas aeruginosa
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.26 Zn2+ zinc-dependent metallopeptidase Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.26 33000
-
-
Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.26 Pseudomonas aeruginosa
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.26 azocasein + H2O
-
Pseudomonas aeruginosa ?
-
?
3.4.24.26 IgG + H2O
-
Pseudomonas aeruginosa ?
-
?
3.4.24.26 N-succinyl-Ala-Ala-Ala-4-nitroanilide + H2O
-
Pseudomonas aeruginosa N-succinyl-Ala-Ala-Ala + 4-nitroaniline
-
?
3.4.24.26 nucleoside diphosphate kinase + H2O
-
Pseudomonas aeruginosa ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.26 elastase B
-
Pseudomonas aeruginosa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.24.26 0.000041
-
N-mercaptoacetyl-Phe-Tyr-amide pH and temperature not specified in the publication Pseudomonas aeruginosa

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.4.24.26 0.0000059
-
pH and temperature not specified in the publication Pseudomonas aeruginosa N-aryl mercaptoacetamide
3.4.24.26 0.00273
-
pH and temperature not specified in the publication Pseudomonas aeruginosa 1-(biphenyl-4-ylmethyl)-3-hydroxy-2-methylpyridine-4(1H)-thione

General Information

EC Number General Information Comment Organism
3.4.24.26 physiological function the enzyme outstands as a pivotal virulence attribute during the infectious process, playing multifunctional roles in different aspects of the pathogen-host interaction. Most abundant peptidase found in pseudomonal secretions, which contributes to the invasiveness of Pseudomonas aeruginosa due to its ability to cleave several extracellular matrix proteins and to disrupt the basolateral intercellular functions present in the host tissues. Pseudolysin makes Pseudomonas aeruginosa able to overcome host defenses by the hydrolysis of many immunologically relevant molecules, including antibodies and complement components Pseudomonas aeruginosa