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Literature summary extracted from

  • Reda, F.
    Kinetic properties of Streptomyces canarius L-glutaminase and its anticancer efficiency (2015), Braz. J. Microbiol., 46, 957-968 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.5.1.38 medicine L-glutaminase has a significant efficiency against Hep-G2 cell (IC50 6.8x02g/ml) and HeLa cells (IC50 8.3 g/ml), while the growth of MCF-7 cells is not affected. L-glutaminase has a moderate cytotoxic effect against HCT-116 cells (IC50 64.7 g/ml) and RAW 264.7 cells (IC50 59.3 g/ml). In vivo, L-glutaminase shows no observed changes in liver, kidney functions, hematological parameters and slight effect on red blood cells and level of platelets after 10 days of rabbit's injection Streptomyces canarius

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.38 0.129
-
L-glutamine pH not specified in the publication, temperature not specified in the publication Streptomyces canarius
3.5.1.38 0.137
-
L-asparagine pH not specified in the publication, temperature not specified in the publication Streptomyces canarius
3.5.1.38 5.38
-
L-aspartate pH not specified in the publication, temperature not specified in the publication Streptomyces canarius

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.38 Streptomyces canarius
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.38
-
Streptomyces canarius

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.38 132.2
-
pH not specified in the publication, temperature not specified in the publication Streptomyces canarius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.38 L-asparagine + H2O
-
Streptomyces canarius L-aspartate + NH3
-
r
3.5.1.38 L-aspartate + NH3
-
Streptomyces canarius L-asparagine + H2O
-
r
3.5.1.38 L-glutamine + H2O
-
Streptomyces canarius L-glutamate + NH3
-
?

Subunits

EC Number Subunits Comment Organism
3.5.1.38 ? x * 44000, SDS-PAGE Streptomyces canarius

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.38 40
-
-
Streptomyces canarius

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.38 57.5
-
melting temperature Streptomyces canarius

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.38 0.00025
-
L-aspartate pH not specified in the publication, temperature not specified in the publication Streptomyces canarius
3.5.1.38 0.00068
-
L-asparagine pH not specified in the publication, temperature not specified in the publication Streptomyces canarius
3.5.1.38 0.00075
-
L-glutamine pH not specified in the publication, temperature not specified in the publication Streptomyces canarius

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.38 8
-
-
Streptomyces canarius

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.38 5 11
-
Streptomyces canarius