EC Number | Application | Comment | Organism |
---|---|---|---|
3.5.1.38 | medicine | L-glutaminase has a significant efficiency against Hep-G2 cell (IC50 6.8x02g/ml) and HeLa cells (IC50 8.3 g/ml), while the growth of MCF-7 cells is not affected. L-glutaminase has a moderate cytotoxic effect against HCT-116 cells (IC50 64.7 g/ml) and RAW 264.7 cells (IC50 59.3 g/ml). In vivo, L-glutaminase shows no observed changes in liver, kidney functions, hematological parameters and slight effect on red blood cells and level of platelets after 10 days of rabbit's injection | Streptomyces canarius |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.38 | 0.129 | - |
L-glutamine | pH not specified in the publication, temperature not specified in the publication | Streptomyces canarius | |
3.5.1.38 | 0.137 | - |
L-asparagine | pH not specified in the publication, temperature not specified in the publication | Streptomyces canarius | |
3.5.1.38 | 5.38 | - |
L-aspartate | pH not specified in the publication, temperature not specified in the publication | Streptomyces canarius |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.38 | Streptomyces canarius | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.1.38 | - |
Streptomyces canarius |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.1.38 | 132.2 | - |
pH not specified in the publication, temperature not specified in the publication | Streptomyces canarius |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.38 | L-asparagine + H2O | - |
Streptomyces canarius | L-aspartate + NH3 | - |
r | |
3.5.1.38 | L-aspartate + NH3 | - |
Streptomyces canarius | L-asparagine + H2O | - |
r | |
3.5.1.38 | L-glutamine + H2O | - |
Streptomyces canarius | L-glutamate + NH3 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.1.38 | ? | x * 44000, SDS-PAGE | Streptomyces canarius |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.38 | 40 | - |
- |
Streptomyces canarius |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.38 | 57.5 | - |
melting temperature | Streptomyces canarius |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.38 | 0.00025 | - |
L-aspartate | pH not specified in the publication, temperature not specified in the publication | Streptomyces canarius | |
3.5.1.38 | 0.00068 | - |
L-asparagine | pH not specified in the publication, temperature not specified in the publication | Streptomyces canarius | |
3.5.1.38 | 0.00075 | - |
L-glutamine | pH not specified in the publication, temperature not specified in the publication | Streptomyces canarius |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.38 | 8 | - |
- |
Streptomyces canarius |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.38 | 5 | 11 | - |
Streptomyces canarius |