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Literature summary extracted from

  • El-Sayed, A.; Hassan, M.; Nada, H.
    Purification, immobilization, and biochemical characterization of L-arginine deiminase from thermophilic Aspergillus fumigatus KJ434941 Anticancer activity in vitro (2015), Biotechnol. Prog., 31, 396-405 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.3.6 drug development L-arginine deiminase has a powerful anticancer activity against various tumors, via arginine depletion, arresting the cell cycle at G1 phase. The free and PEGylated enzyme exhibits a similar cytotoxic efficacy against HCT, HEP-G2, and MCF7 cells, lower than the cytotoxic efficacy of to enzyme covalently immobilized on dextran. The in vitro anticancer activity of the enzyme against HCT, MCF7, and HEPG-2 cells is increased by five-, three-, and threefold upon covalent modification by dextran Aspergillus fumigatus

General Stability

EC Number General Stability Organism
3.5.3.6 upon proteolysis for 30 min, the residual activity of the soluble enzyme, the PEGylated enzyme, and the enzyme covalently immobilized on dextran is 8.0%, 32.0%, and 20.0% for proteinase K and 10.0%, 52.0%, and 90.0% for acid protease, respectively Aspergillus fumigatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.3.6 6-diazo-5-oxo-norleucine
-
 Aspergillus fumigatus
3.5.3.6 hydroxylamine
-
 Aspergillus fumigatus
3.5.3.6 iodoacetate
-
 Aspergillus fumigatus
3.5.3.6 propargylglycine
-
 Aspergillus fumigatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.6 8.66
-
 L-alanine pH 7.0, 45°C, soluble enzyme Aspergillus fumigatus
3.5.3.6 8.76
-
 L-arginine pH 7.0, 45°C, soluble enzyme Aspergillus fumigatus
3.5.3.6 12.34
-
 L-lysine pH 7.0, 45°C, soluble enzyme Aspergillus fumigatus
3.5.3.6 15.48
-
 L-lysine pH 7.0, 45°C, enzyme covalently immobilized on dextran Aspergillus fumigatus
3.5.3.6 15.48
-
 L-alanine pH 7.0, 45°C, PEGylated enzyme Aspergillus fumigatus
3.5.3.6 15.57
-
 L-arginine pH 7.0, 45°C, PEGylated enzyme Aspergillus fumigatus
3.5.3.6 18.42
-
 L-lysine pH 7.0, 45°C, PEGylated enzyme Aspergillus fumigatus
3.5.3.6 22.52
-
 L-arginine pH 7.0, 45°C, enzyme covalently immobilized on dextran Aspergillus fumigatus
3.5.3.6 23.21
-
 L-alanine pH 7.0, 45°C, enzyme covalently immobilized on dextran Aspergillus fumigatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.6 100000 110000 gel filtration Aspergillus fumigatus

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.6 Aspergillus fumigatus
-
-
-
3.5.3.6 Aspergillus fumigatus KJ434941
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.6
-
 Aspergillus fumigatus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.3.6 18
-
 pH 7.0, 45°C, enzyme covalently immobilized on dextran Aspergillus fumigatus
3.5.3.6 21.5
-
 pH 7.0, 45°C, PEGylated enzyme Aspergillus fumigatus
3.5.3.6 26.7
-
 pH 7.0, 45°C, soluble enzyme Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.6 L-alanine + H2O
-
 Aspergillus fumigatus ? + NH3
-
 ?
3.5.3.6 L-alanine + H2O
-
 Aspergillus fumigatus KJ434941 ? + NH3
-
 ?
3.5.3.6 L-arginine + H2O
-
 Aspergillus fumigatus L-citrulline + NH3
-
 ?
3.5.3.6 L-arginine + H2O
-
 Aspergillus fumigatus KJ434941 L-citrulline + NH3
-
 ?
3.5.3.6 L-lysine + H2O
-
 Aspergillus fumigatus ? + NH3
-
 ?
3.5.3.6 L-lysine + H2O
-
 Aspergillus fumigatus KJ434941 ? + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.3.6 homodimer 2 * 50000, SDS-PAGE Aspergillus fumigatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.3.6 40
-
 soluble enzyme (t1/2: 12.1 h), EGylated enzyme (t1/2: 14.6 h), enzyme covalently immobilized on dextran (13.9 h) Aspergillus fumigatus
3.5.3.6 50
-
 PEGylated enzyme displays twofold resistance to thermal denaturation (t1/2: 13.9 h) compared to soluble enzyme (t1/2: 8.3 h) Aspergillus fumigatus
3.5.3.6 60
-
 soluble enzyme (t1/2: 5.7 h), EGylated enzyme (t1/2: 12.1 h), enzyme covalently immobilized on dextran (12.1 h) Aspergillus fumigatus
3.5.3.6 70
-
 thermal stability of PEGylated enzyme (9.1 h) is increased by 1.7fold. Similar stability to enzyme covalently immobilized on dextran (5.7 h) with the soluble enzyme (5.6 h) Aspergillus fumigatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.3.6 0.0024
-
 L-alanine pH 7.0, 45°C, soluble enzyme Aspergillus fumigatus
3.5.3.6 0.0033
-
 L-lysine pH 7.0, 45°C, enzyme covalently immobilized on dextran Aspergillus fumigatus
3.5.3.6 0.0034
-
 L-alanine pH 7.0, 45°C, PEGylated enzyme Aspergillus fumigatus
3.5.3.6 0.004
-
 L-lysine pH 7.0, 45°C, soluble enzyme Aspergillus fumigatus
3.5.3.6 0.0041
-
 L-arginine pH 7.0, 45°C, enzyme covalently immobilized on dextran Aspergillus fumigatus
3.5.3.6 0.0043
-
 L-lysine pH 7.0, 45°C, PEGylated enzyme Aspergillus fumigatus
3.5.3.6 0.00475
-
 L-arginine pH 7.0, 45°C, PEGylated enzyme Aspergillus fumigatus
3.5.3.6 0.00478
-
 L-arginine pH 7.0, 45°C, soluble enzyme Aspergillus fumigatus
3.5.3.6 0.006
-
 L-alanine pH 7.0, 45°C, enzyme covalently immobilized on dextran Aspergillus fumigatus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.3.6 0.343
-
 hydroxylamine pH 7.0, 45°C, soluble enzyme Aspergillus fumigatus
3.5.3.6 0.387
-
 iodoacetate pH 7.0, 45°C, soluble enzyme Aspergillus fumigatus
3.5.3.6 0.4511
-
 iodoacetate pH 7.0, 45°C, PEGylated enzyme Aspergillus fumigatus
3.5.3.6 0.481
-
 propargylglycine pH 7.0, 45°C, soluble enzyme Aspergillus fumigatus
3.5.3.6 0.615
-
 hydroxylamine pH 7.0, 45°C, enzyme covalently immobilized on dextran Aspergillus fumigatus
3.5.3.6 0.783
-
 propargylglycine pH 7.0, 45°C, enzyme covalently immobilized on dextran Aspergillus fumigatus
3.5.3.6 1.164
-
 hydroxylamine pH 7.0, 45°C, PEGylated enzyme Aspergillus fumigatus
3.5.3.6 1.215
-
 6-diazo-5-oxo-norleucine pH 7.0, 45°C, soluble enzyme Aspergillus fumigatus
3.5.3.6 2.081
-
 6-diazo-5-oxo-norleucine pH 7.0, 45°C, enzyme covalently immobilized on dextran Aspergillus fumigatus
3.5.3.6 2.232
-
 6-diazo-5-oxo-norleucine pH 7.0, 45°C, PEGylated enzyme Aspergillus fumigatus
3.5.3.6 2.291
-
 iodoacetate pH 7.0, 45°C, enzyme covalently immobilized on dextran Aspergillus fumigatus
3.5.3.6 2.739
-
 propargylglycine pH 7.0, 45°C, PEGylated enzyme Aspergillus fumigatus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.5.3.6 1.9
-
 pH 7.0, 45°C, PEGylated enzyme Aspergillus fumigatus iodoacetate
3.5.3.6 1.98
-
 pH 7.0, 45°C, enzyme covalently immobilized on dextran Aspergillus fumigatus hydroxylamine
3.5.3.6 2.3
-
 pH 7.0, 45°C, soluble enzyme Aspergillus fumigatus hydroxylamine
3.5.3.6 2.52
-
 pH 7.0, 45°C, enzyme covalently immobilized on dextran Aspergillus fumigatus propargylglycine
3.5.3.6 2.6
-
 pH 7.0, 45°C, soluble enzyme Aspergillus fumigatus iodoacetate
3.5.3.6 3.22
-
 pH 7.0, 45°C, soluble enzyme Aspergillus fumigatus propargylglycine
3.5.3.6 4.9
-
 pH 7.0, 45°C, PEGylated enzyme Aspergillus fumigatus hydroxylamine
3.5.3.6 6.7
-
 pH 7.0, 45°C, enzyme covalently immobilized on dextran Aspergillus fumigatus 6-diazo-5-oxo-norleucine
3.5.3.6 7.39
-
 pH 7.0, 45°C, enzyme covalently immobilized on dextran Aspergillus fumigatus iodoacetate
3.5.3.6 8.15
-
 pH 7.0, 45°C, soluble enzyme Aspergillus fumigatus 6-diazo-5-oxo-norleucine
3.5.3.6 9.4
-
 pH 7.0, 45°C, PEGylated enzyme Aspergillus fumigatus 6-diazo-5-oxo-norleucine
3.5.3.6 11.54
-
 pH 7.0, 45°C, PEGylated enzyme Aspergillus fumigatus propargylglycine

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.3.6 0.00018
-
 L-arginine pH 7.0, 45°C, enzyme covalently immobilized on dextran Aspergillus fumigatus
3.5.3.6 0.000215
-
 L-lysine pH 7.0, 45°C, enzyme covalently immobilized on dextran Aspergillus fumigatus
3.5.3.6 0.000215
-
 L-alanine pH 7.0, 45°C, PEGylated enzyme Aspergillus fumigatus
3.5.3.6 0.000235
-
 L-lysine pH 7.0, 45°C, PEGylated enzyme Aspergillus fumigatus
3.5.3.6 0.000258
-
 L-alanine pH 7.0, 45°C, enzyme covalently immobilized on dextran Aspergillus fumigatus
3.5.3.6 0.000277
-
 L-alanine pH 7.0, 45°C, soluble enzyme Aspergillus fumigatus
3.5.3.6 0.0003
-
 L-arginine pH 7.0, 45°C, PEGylated enzyme Aspergillus fumigatus
3.5.3.6 0.00032
-
 L-lysine pH 7.0, 45°C, soluble enzyme Aspergillus fumigatus
3.5.3.6 0.00055
-
 L-arginine pH 7.0, 45°C, soluble enzyme Aspergillus fumigatus