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Literature summary extracted from

  • Husain, I.; Sharma, A.; Kumar, S.; Malik, F.
    Purification and characterization of glutaminase free asparaginase from Pseudomonas otitidis Induce apoptosis in human leukemia MOLT-4 cells (2016), Biochimie, 121, 38-51 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.1 medicine the anticancer activity of purified asparaginase is comparable or higher than that of commercial Escherichia coli asparaginase. The purified enzyme induces apoptotic cell death in dose-dependent manner, probybly via activation of anintrinsic apoptotic pathway. The purified enzyme is nontoxic for human noncancerous FR-2 cells and human blood lymphocytes Pseudomonas otitidis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.1 Ca2+ 150 mM, about 75% of initial activity Pseudomonas otitidis
3.5.1.1 Cd2+ 10 mM, loss of activity Pseudomonas otitidis
3.5.1.1 EDTA 5 mM, about 85% residual activity Pseudomonas otitidis
3.5.1.1 Fe3+ 100 mM, about 55% of initial activity Pseudomonas otitidis
3.5.1.1 Hg2+ 100 mM, loss of activity Pseudomonas otitidis
3.5.1.1 Mg2+ 40 mM, about 30% of initial activity Pseudomonas otitidis
3.5.1.1 Mn2+ 100 mM, about 75% of initial activity Pseudomonas otitidis
3.5.1.1 Ni2+ 10 mM, loss of activity Pseudomonas otitidis
3.5.1.1 SDS 2 mM, about 55% residual activity Pseudomonas otitidis
3.5.1.1 Thiourea 1 mM, about 85% residual activtiy Pseudomonas otitidis
3.5.1.1 Zn2+ 100 mM, about 60% of initial activity Pseudomonas otitidis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.1 K+ 150 mM, about 130% of initial activity Pseudomonas otitidis
3.5.1.1 Na+ 50 mM, about 115% of initial activity Pseudomonas otitidis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.1 205000
-
 PAGE Pseudomonas otitidis

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Pseudomonas otitidis A0A1I0U0N3
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.1 107.84
-
 pH 8.6, 37°C Pseudomonas otitidis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 L-asparagine + H2O
-
 Pseudomonas otitidis L-aspartate + NH3
-
 ?
3.5.1.1 additional information poor or no substrates: D-asparagine, L-glutamine, D-glutamine, L-histidine, L-ornithine, Boc-L-asapragine, N-alpha-acetyl-L-asparagine, urea, acrylamide Pseudomonas otitidis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.1 hexamer 6 * 34000, SDS-PAGE Pseudomonas otitidis

Synonyms

EC Number Synonyms Comment Organism
3.5.1.1 SAMN05216263_10744
-
 Pseudomonas otitidis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.1 40
-
-
 Pseudomonas otitidis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.1 7.5
-
-
 Pseudomonas otitidis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.5.1.1 Pseudomonas otitidis isoelectric focussing
-
 5.5