Literature summary extracted from
Schacherl, M.; Gompert, M.; Pardon, E.; Lamkemeyer, T.; Steyaert, J.; Baumann, U.
Crystallographic and biochemical characterization of the dimeric architecture of site-2 protease (2017), Biochim. Biophys. Acta Biomembr., 1859, 1859-1871 .
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.4.24.85 |
expressed in Escherichia coli C43 (DE3) cells for full-length protein and BL21 (DE3) pLysS (DE3) for the CBS domain |
Archaeoglobus fulgidus |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
3.4.24.85 |
regulatory CBS domain pair of the enzyme in the apo and nucleotide-bound form, sitting drop vapor diffusion method, using 0.1 M Bis-Tris pH 6.5, 0.2 M (NH4)2SO4, 20% (w/v) PEG 3350 |
Archaeoglobus fulgidus |
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
3.4.24.85 |
membrane |
- |
Archaeoglobus fulgidus |
16020 |
- |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.4.24.85 |
Zn2+ |
contains zinc |
Archaeoglobus fulgidus |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.4.24.85 |
Archaeoglobus fulgidus |
O29915 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.4.24.85 |
Ni-NTA column chromatography, Strep-Tactin column chromatography, and Superdex S200 gel filtration |
Archaeoglobus fulgidus |
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.4.24.85 |
homodimer |
x-ray crystallography |
Archaeoglobus fulgidus |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.4.24.85 |
MEROPS:M50 |
- |
Archaeoglobus fulgidus |
3.4.24.85 |
S2P |
- |
Archaeoglobus fulgidus |
3.4.24.85 |
site-2 protease |
- |
Archaeoglobus fulgidus |