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Literature summary extracted from

  • Hu, X.; Compton, J.R.; Leary, D.H.; Olson, M.A.; Lee, M.S.; Cheung, J.; Ye, W.; Ferrer, M.; Southall, N.; Jadhav, A.; Morazzani, E.M.; Glass, P.J.; Marugan, J.; Legler, P.M.
    Kinetic, mutational, and structural studies of the Venezuelan equine encephalitis virus nonstructural protein 2 cysteine protease (2016), Biochemistry, 55, 3007-3019 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.B79 expression in Escherichia coli Venezuelan equine encephalitis virus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.22.B79 free enzyme and adduct with inhibitor E64d Venezuelan equine encephalitis virus

Protein Variants

EC Number Protein Variants Comment Organism
3.4.22.B79 K480A mutation leads to a 9fold decrease in kcat and kcat/Km. K480 likely enhances the nucleophilicity of the Cys residue Venezuelan equine encephalitis virus
3.4.22.B79 K706A SAM MTase domain mutation, increases Km 4.5fold to 500 microM Venezuelan equine encephalitis virus
3.4.22.B79 N475A mutation leads to a 24fold reduction in kcat/Km, and the conformation of this residue does not change after inhibition. Residue N475 forms the base of the P1 binding site and likely orients the substrate for nucleophilic attack or plays a role in product release Venezuelan equine encephalitis virus
3.4.22.B79 N545A mutation within the beta-hairpin, lightly but not significantly increases kcat and Km Venezuelan equine encephalitis virus
3.4.22.B79 R662A mutation leads to 16fold decreases in kcat/Km Venezuelan equine encephalitis virus
3.4.22.B79 R662K mutation leads to 16fold decreases in kcat/Km Venezuelan equine encephalitis virus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.B79 E64d inhibitor binds beneath a beta-hairpin at the interface of the SAM MTase and protease domains Venezuelan equine encephalitis virus
3.4.22.B79 additional information E64c, the carboxylic acid form of the E64d ester, does not inhibit the nsP2 protease Venezuelan equine encephalitis virus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.B79 Venezuelan equine encephalitis virus P27282
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3.4.22.B79 Venezuelan equine encephalitis virus Trinidad donkey P27282
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