Literature summary extracted from
Hu, X.; Compton, J.R.; Leary, D.H.; Olson, M.A.; Lee, M.S.; Cheung, J.; Ye, W.; Ferrer, M.; Southall, N.; Jadhav, A.; Morazzani, E.M.; Glass, P.J.; Marugan, J.; Legler, P.M.
Kinetic, mutational, and structural studies of the Venezuelan equine encephalitis virus nonstructural protein 2 cysteine protease (2016), Biochemistry, 55, 3007-3019 .
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.4.22.B79 |
expression in Escherichia coli |
Venezuelan equine encephalitis virus |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
3.4.22.B79 |
free enzyme and adduct with inhibitor E64d |
Venezuelan equine encephalitis virus |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
3.4.22.B79 |
K480A |
mutation leads to a 9fold decrease in kcat and kcat/Km. K480 likely enhances the nucleophilicity of the Cys residue |
Venezuelan equine encephalitis virus |
3.4.22.B79 |
K706A |
SAM MTase domain mutation, increases Km 4.5fold to 500 microM |
Venezuelan equine encephalitis virus |
3.4.22.B79 |
N475A |
mutation leads to a 24fold reduction in kcat/Km, and the conformation of this residue does not change after inhibition. Residue N475 forms the base of the P1 binding site and likely orients the substrate for nucleophilic attack or plays a role in product release |
Venezuelan equine encephalitis virus |
3.4.22.B79 |
N545A |
mutation within the beta-hairpin, lightly but not significantly increases kcat and Km |
Venezuelan equine encephalitis virus |
3.4.22.B79 |
R662A |
mutation leads to 16fold decreases in kcat/Km |
Venezuelan equine encephalitis virus |
3.4.22.B79 |
R662K |
mutation leads to 16fold decreases in kcat/Km |
Venezuelan equine encephalitis virus |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.4.22.B79 |
E64d |
inhibitor binds beneath a beta-hairpin at the interface of the SAM MTase and protease domains |
Venezuelan equine encephalitis virus |
|
3.4.22.B79 |
additional information |
E64c, the carboxylic acid form of the E64d ester, does not inhibit the nsP2 protease |
Venezuelan equine encephalitis virus |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.4.22.B79 |
Venezuelan equine encephalitis virus |
P27282 |
- |
- |
3.4.22.B79 |
Venezuelan equine encephalitis virus Trinidad donkey |
P27282 |
- |
- |