EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.14.11.65 | tetrahydrofolate | binding kinetic analysis of folate derivatives, e.g. folates bound to glutamates, the (6R,S) form of the natural pentaglutamate form of tetrahydrofolate bound with the highest affinity to full-length LSD1, binding of different forms of folate to LSD1, and binding structures, overview | Homo sapiens | |
1.14.99.66 | tetrahydrofolate | binding kinetic analysis of folate derivatives, e.g. folates bound to glutamates, the (6R,S) form of the natural pentaglutamate form of tetrahydrofolate bound with the highest affinity to full-length LSD1, binding of different forms of folate to LSD1, and binding structures, overview | Homo sapiens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.11.65 | recombinant expression of N-terminally His-tagged enzyme in Escherichia coli BL21(DE3), recombinant expression of N-terminally truncated GST-tagged LSD1 | Homo sapiens |
1.14.99.66 | recombinant expression of N-terminally His-tagged enzyme in Escherichia coli BL21(DE3), recombinant expression of N-terminally truncated GST-tagged LSD1 | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.11.65 | nucleus | LSD1 is associated with folate | Homo sapiens | 5634 | - |
1.14.99.66 | nucleus | LSD1 is associated with folate | Homo sapiens | 5634 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.11.65 | 100000 | - |
about, recombinant enzyme, gel filtration | Homo sapiens |
1.14.99.66 | 100000 | - |
about, recombinant enzyme, gel filtration | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.11.65 | [histone H3]-N6,N6-dimethyl-L-lysine 9 + 2-oxoglutarate + O2 | Homo sapiens | - |
[histone H3]-N6-methyl-L-lysine 9 + succinate + formaldehyde + CO2 | - |
? | |
1.14.11.65 | [histone H3]-N6-methyl-L-lysine 9 + 2-oxoglutarate + O2 | Homo sapiens | - |
[histone H3]-L-lysine 9 + succinate + formaldehyde + CO2 | - |
? | |
1.14.99.66 | [histone H3]-N6,N6-dimethyl-L-lysine 4 + 2-oxoglutarate + O2 | Homo sapiens | - |
[histone H3]-N6-methyl-L-lysine 4 + succinate + formaldehyde + CO2 | - |
? | |
1.14.99.66 | [histone H3]-N6-methyl-L-lysine 4 + 2-oxoglutarate + O2 | Homo sapiens | - |
[histone H3]-L-lysine 4 + succinate + formaldehyde + CO2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.11.65 | Homo sapiens | O60341 | - |
- |
1.14.99.66 | Homo sapiens | O60341 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.11.65 | recombinant N-terminally His-tagged enzyme by nickel affinity chromatography from Escherichia coli BL21(DE3), or by ammonium sulfate fractionation and anion exchange chromatography, recombinant N-terminally truncated GST-tagged LSD1 by glutathione affinity chromatography | Homo sapiens |
1.14.99.66 | recombinant N-terminally His-tagged enzyme by nickel affinity chromatography from Escherichia coli BL21(DE3), or by ammonium sulfate fractionation and anion exchange chromatography, recombinant N-terminally truncated GST-tagged LSD1 by glutathione affinity chromatography | Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.11.65 | HeLa cell | nuclear extracts of HeLa cells contain LSD1 that is associated with folate | Homo sapiens | - |
1.14.99.66 | HeLa cell | nuclear extracts of HeLa cells contain LSD1 that is associated with folate | Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.11.65 | additional information | the bifunctional enzyme catalyzes the demethylation of H3K9me2/me1 and H3K4me2/me1 (1.14.99.66) | Homo sapiens | ? | - |
? | |
1.14.11.65 | [histone H3]-N6,N6-dimethyl-L-lysine 9 + 2-oxoglutarate + O2 | - |
Homo sapiens | [histone H3]-N6-methyl-L-lysine 9 + succinate + formaldehyde + CO2 | - |
? | |
1.14.11.65 | [histone H3]-N6-methyl-L-lysine 9 + 2-oxoglutarate + O2 | - |
Homo sapiens | [histone H3]-L-lysine 9 + succinate + formaldehyde + CO2 | - |
? | |
1.14.99.66 | additional information | the bifunctional enzyme catalyzes the demethylation of H3K9me2/me1 (EC 1.14.11.65) and H3K4me2/me1 | Homo sapiens | ? | - |
? | |
1.14.99.66 | [histone H3]-N6,N6-dimethyl-L-lysine 4 + 2-oxoglutarate + O2 | - |
Homo sapiens | [histone H3]-N6-methyl-L-lysine 4 + succinate + formaldehyde + CO2 | - |
? | |
1.14.99.66 | [histone H3]-N6-methyl-L-lysine 4 + 2-oxoglutarate + O2 | - |
Homo sapiens | [histone H3]-L-lysine 4 + succinate + formaldehyde + CO2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.11.65 | monomer | 1 * 95059, LSD1 without N-terminal methionine, mass spectrometry | Homo sapiens |
1.14.99.66 | monomer | 1 * 95059, LSD1 without N-terminal methionine, mass spectrometry | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.11.65 | LSD1 | - |
Homo sapiens |
1.14.11.65 | More | see also for EC 1.14.99.66 | Homo sapiens |
1.14.99.66 | LSD1 | - |
Homo sapiens |
1.14.99.66 | More | see also for EC 1.14.11.65 | Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.11.65 | 22 | - |
assay at room temperature | Homo sapiens |
1.14.99.66 | 22 | - |
assay at room temperature | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.11.65 | 7.2 | 7.5 | assay at | Homo sapiens |
1.14.99.66 | 7.2 | 7.5 | assay at | Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.11.65 | FAD | required, a flavin-containing enzyme | Homo sapiens | |
1.14.99.66 | FAD | required, a flavin-containing enzyme | Homo sapiens |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.11.65 | additional information | mechanism of histone H3 demethylation by demethylase LSD1, overview | Homo sapiens |
1.14.11.65 | physiological function | methylation on the N-terminal tails of histone lysines serves as an epigenetic control mechanism, which is regulated by demathylases | Homo sapiens |
1.14.99.66 | additional information | mechanism of histone H3 demethylation by demethylase LSD1, overview | Homo sapiens |
1.14.99.66 | physiological function | methylation on the N-terminal tails of histone lysines serves as an epigenetic control mechanism, which is regulated by demathylases | Homo sapiens |