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Literature summary extracted from

  • Pfleger, B.F.; Lee, J.Y.; Somu, R.V.; Aldrich, C.C.; Hanna, P.C.; Sherman, D.H.
    Characterization and analysis of early enzymes for petrobactin biosynthesis in Bacillus anthracis (2007), Biochemistry, 46, 4147-4157 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.62 expression in Escherichia coli BLR(DE3) Bacillus anthracis

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.62 3,4-dihydroxybenzoate substrate inhibition Bacillus anthracis
6.2.1.62 ATP substrate inhibition Bacillus anthracis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.62 0.0031
-
 3,4-dihydroxybenzoate pH 7.5, temperature not specified in the publication Bacillus anthracis
6.2.1.62 0.216
-
 ATP pH 7.5, temperature not specified in the publication Bacillus anthracis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.62 47000
-
 His-tagged enzyme, SDS-PAGE Bacillus anthracis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.62 ATP + 3,4-dihydroxybenzoate + holo-[aryl-carrier protein] Bacillus anthracis the enzyme is involved in petrobactin biosynthesis AMP + diphosphate + 3,4-dihydroxybenzoyl-[aryl-carrier protein]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.62 Bacillus anthracis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.62
-
 Bacillus anthracis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.62 (3,4-dihydroxybenzoyl)adenylate + holo-[aryl-carrier protein] the adenylation domain of the enzyme catalyses the activation of 3,4-dihydroxybenzoate to (3,4-dihydroxybenzoyl)adenylate, followed by the transfer of the activated compound to the free thiol of a phosphopantetheine arm of an aryl-carrier protein domain Bacillus anthracis AMP + 3,4-dihydroxybenzoyl-[aryl-carrier protein]
-
 ?
6.2.1.62 ATP + 3,4-dihydroxybenzoate the adenylation domain of the enzyme catalyses the activation of 3,4-dihydroxybenzoate to (3,4-dihydroxybenzoyl)adenylate, followed by the transfer of the activated compound to the free thiol of a phosphopantetheine arm of an aryl-carrier protein domain Bacillus anthracis diphosphate + (3,4-dihydroxybenzoyl)adenylate
-
 ?
6.2.1.62 ATP + 3,4-dihydroxybenzoate + holo-[aryl-carrier protein] the enzyme is involved in petrobactin biosynthesis Bacillus anthracis AMP + diphosphate + 3,4-dihydroxybenzoyl-[aryl-carrier protein]
-
 ?
6.2.1.62 ATP + 3,4-dihydroxybenzoate + holo-[aryl-carrier protein] the adenylation domain of the enzyme catalyses the activation of 3,4-dihydroxybenzoate to (3,4-dihydroxybenzoyl)adenylate, followed by the transfer of the activated compound to the free thiol of a phosphopantetheine arm of an aryl-carrier protein domain Bacillus anthracis AMP + diphosphate + 3,4-dihydroxybenzoyl-[aryl-carrier protein]
-
 ?
6.2.1.62 ATP + 3,5-dihydroxybenzoic acid + holo-[aryl-carrier protein] about 20% of the activity compared to 3,4-dihydroxybenzoate Bacillus anthracis AMP + diphosphate + 3,5-dihydroxybenzoyl-[aryl-carrier protein]
-
 ?
6.2.1.62 ATP + 3-chloro-4-hydroxybenzoic acid + holo-[aryl-carrier protein] about 5% of the activity compared to 3,4-dihydroxybenzoate Bacillus anthracis AMP + diphosphate + 3-chloro-4-hydroxybenzoyl-[aryl-carrier protein]
-
 ?
6.2.1.62 ATP + 3-hydroxybenzoic acid + holo-[aryl-carrier protein] about 5% of thze activity compared to 3,4-dihydroxybenzoate Bacillus anthracis AMP + diphosphate + 3-hydroxybenzoyl-[aryl-carrier protein]
-
 ?
6.2.1.62 ATP + 4-fluoro-3-hydroxybenzoic acid + holo-[aryl-carrier protein] about 5% of the activity compared to 3,4-dihydroxybenzoate Bacillus anthracis AMP + diphosphate + 4-fluoro-3-hydroxybenzoyl-[aryl-carrier protein]
-
 ?
6.2.1.62 ATP + 4-hydroxybenzoic acid + holo-[aryl-carrier protein] about 60% of the activity compared to 3,4-dihydroxybenzoate Bacillus anthracis AMP + diphosphate + 4-hydroxybenzoyl-[aryl-carrier protein]
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.62 ? x * 47000, His-tagged enzyme, SDS-PAGE Bacillus anthracis

Synonyms

EC Number Synonyms Comment Organism
6.2.1.62 3,4-dihydroxybenzoic acid-AMP ligase
-
 Bacillus anthracis
6.2.1.62 asbC
-
 Bacillus anthracis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2.1.62 0.118
-
 3,4-dihydroxybenzoate pH 7.5, temperature not specified in the publication Bacillus anthracis
6.2.1.62 0.152
-
 ATP pH 7.5, temperature not specified in the publication Bacillus anthracis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.2.1.62 0.035
-
 3,4-dihydroxybenzoate pH 7.5, temperature not specified in the publication Bacillus anthracis
6.2.1.62 54
-
 ATP pH 7.5, temperature not specified in the publication Bacillus anthracis

General Information

EC Number General Information Comment Organism
6.2.1.62 physiological function the enzyme is involved in petrobactin biosynthesis Bacillus anthracis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.2.1.62 0.7
-
 ATP pH 7.5, temperature not specified in the publication Bacillus anthracis
6.2.1.62 30.06
-
 3,4-dihydroxybenzoate pH 7.5, temperature not specified in the publication Bacillus anthracis