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Literature summary extracted from

  • Marui, J.; Matsushita-Morita, M.; Tada, S.; Hattori, R.; Suzuki, S.; Amano, H.; Ishida, H.; Yamagata, Y.; Takeuchi, M.; Kusumoto, K.
    Enzymatic properties of the glycine D-alanine aminopeptidase of Aspergillus oryzae and its activity profiles in liquid-cultured mycelia and solid-state rice culture (rice koji) (2012), Appl. Microbiol. Biotechnol., 93, 655-669 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.19 AEBSF
-
 Aspergillus oryzae
3.4.11.19 benzamidine
-
 Aspergillus oryzae
3.4.11.19 additional information not inhibitory: bestatin Aspergillus oryzae
3.4.11.19 PCMB
-
 Aspergillus oryzae
3.4.11.19 TLCK
-
 Aspergillus oryzae
3.4.11.19 TPCK
-
 Aspergillus oryzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.19 126300
-
 gel filtration Aspergillus oryzae

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.19 Aspergillus oryzae G7H7Y1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.19 recombinant protein, purified GdaA might be digested during the final step of purification without affecting the activity and dimerization under nondenaturing conditions Aspergillus oryzae

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.19 D-Ala 4-nitroanilide + H2O 93% of the activity with Gly 4-nitroanilide Aspergillus oryzae D-Ala + 4-nitroaniline
-
 ?
3.4.11.19 D-Ala-D-Ala + H2O
-
 Aspergillus oryzae 2 D-Ala
-
 ?
3.4.11.19 D-Ala-Gly + H2O
-
 Aspergillus oryzae D-Ala + Gly
-
 ?
3.4.11.19 D-Ala-Gly-Gly + H2O
-
 Aspergillus oryzae D-Ala + Gly-Gly
-
 ?
3.4.11.19 D-Ala-L-Ala + H2O
-
 Aspergillus oryzae D-Ala + L-Ala
-
 ?
3.4.11.19 Gly 4-nitroanilide + H2O
-
 Aspergillus oryzae Gly + 4-nitroaniline
-
 ?
3.4.11.19 Gly-Gly + H2O
-
 Aspergillus oryzae Gly + Gly
-
 ?
3.4.11.19 Gly-Gly-Gly + 2 H2O
-
 Aspergillus oryzae 3 Gly
-
 ?
3.4.11.19 Gly-L-Ala + H2O
-
 Aspergillus oryzae Gly + L-Ala
-
 ?
3.4.11.19 Gly-L-Cys + H2O
-
 Aspergillus oryzae Gly + L-Cys
-
 ?
3.4.11.19 Gly-L-Cys-Gly + H2O
-
 Aspergillus oryzae Gly + L-Cys-Gly
-
 ?
3.4.11.19 L-Ala 4-nitroanilide + H2O 12% of the activity with Gly 4-nitroanilide Aspergillus oryzae L-Ala + 4-nitroaniline
-
 ?
3.4.11.19 additional information substrates beta-Ala-4-nitroanilide, L-Leu-4-nitroanilide, D-Leu-4-nitroanilide, L-Met-4-nitroanilide, L-Pro-4-nitroanilide, L-Val-4-nitroanilide, L-Ile-4-nitroanilide, L-Phe-4-nitroanilide, D-Phe-4-nitroanilide, L-Lys-4-nitroanilide, L-Arg-4-nitroanilide, L-His-4-nitroanilide, L-Asp-4-nitroanilide, L-Glu-4-nitroanilide show less than 0.5% of the activity with Gly-4-nitroanilide, no substrates: L-Ala-Gly and L-Cys-Gly dipeptides and L-Cys-Gly-Gly tripeptide Aspergillus oryzae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.19 dimer 2 * 59300, calculated from sequence Aspergillus oryzae

Synonyms

EC Number Synonyms Comment Organism
3.4.11.19 GdaA
-
 Aspergillus oryzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.19 40
-
-
 Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.19 8 9
-
 Aspergillus oryzae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.11.19 5 11
-
 Aspergillus oryzae

General Information

EC Number General Information Comment Organism
3.4.11.19 physiological function GdaA is largely responsible for intracellular glycine and D-alanine aminopeptidase activities in Aspergillus oryzae during stationary-phase growth in liquid media. In addition, the activity increases in response to the depletion of nitrogen or carbon sources in the growth media. No significant differences are observed in the vegetative growth and conidiation on minimal and complete media in the GdaA deletion or overexpression strains, and control strains Aspergillus oryzae