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Literature summary extracted from

  • Nakae, S.; Hijikata, A.; Tsuji, T.; Yonezawa, K.; Kouyama, K.I.; Mayanagi, K.; Ishino, S.; Ishino, Y.; Shirai, T.
    Structure of the EndoMS-DNA complex as mismatch restriction endonuclease (2016), Structure, 24, 1960-1971 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.21.B3 mutant enzyme D165A Thermococcus kodakarensis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.21.B3 the structures of TkoEndoMS in complex with dsDNA substrates and in unbound (apo) form are determined at a maximum 2.4 A resolution with X-ray crystallography. The crystals of apo TkoEndoMS are grown in a stock solution (50 mM Tris-HCl [pH 8.0], 0.1 mM EDTA, 0.5 mM DDT, 10% [w/v] glycerol, 0.6 M NaCl, and 445 mM TkoEndoMS) stored at 4°C. The DNA cleavage reaction of the enzyme in the crystals is inhibited by replacing Asp165, one of the putative catalytic sites, with Ala Thermococcus kodakarensis

Protein Variants

EC Number Protein Variants Comment Organism
3.1.21.B3 D165A the DNA cleavage reaction of the enzyme in the crystals is inhibited by replacing Asp165, one of the putative catalytic sites, with Ala Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.21.B3 DNA + H2O Thermococcus kodakarensis the enzyme acts in mismatch repair ?
-
 ?
3.1.21.B3 DNA + H2O Thermococcus kodakarensis ATCC BAA-918 the enzyme acts in mismatch repair ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.B3 Thermococcus kodakarensis Q5JER9
-
-
3.1.21.B3 Thermococcus kodakarensis ATCC BAA-918 Q5JER9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.21.B3 mutant enzyme D165A Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.B3 DNA + H2O the enzyme acts in mismatch repair Thermococcus kodakarensis ?
-
 ?
3.1.21.B3 DNA + H2O the enzyme specifically cleaves double-stranded DNA containing mismatched bases. The complex structure of the enzyme dimer with dsDNA reveals that the mismatched bases are flipped out into binding sites Thermococcus kodakarensis ?
-
 ?
3.1.21.B3 DNA + H2O the enzyme acts in mismatch repair Thermococcus kodakarensis ATCC BAA-918 ?
-
 ?
3.1.21.B3 DNA + H2O the enzyme specifically cleaves double-stranded DNA containing mismatched bases. The complex structure of the enzyme dimer with dsDNA reveals that the mismatched bases are flipped out into binding sites Thermococcus kodakarensis ATCC BAA-918 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.21.B3 endonuclease NucS
-
 Thermococcus kodakarensis
3.1.21.B3 TK1898
-
 Thermococcus kodakarensis
3.1.21.B3 TkEndoMS
-
 Thermococcus kodakarensis

General Information

EC Number General Information Comment Organism
3.1.21.B3 physiological function the enzyme acts in mismatch repair Thermococcus kodakarensis