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Literature summary extracted from

  • Malbert, Y.; Moulis, C.; Brison, Y.; Morel, S.; Andre, I.; Remaud-Simeon, M.
    Engineering a branching sucrase for flavonoid glucoside diversification (2018), Sci. Rep., 8, 15153 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.4.1.373 synthesis use of enzyme to generate a platform for flavonoid glucosylation. Engineered variants show remarkable ability for luteolin, morin and naringenin glucosylation with conversion ranging from 30% to 90% Leuconostoc mesenteroides

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.B77 additional information engineering of truncated variant DELTAN123-GBD-CD2 for flavonoid glucoside diversification. Mutantions at residues W2135, F2136, F2163 and L2166 lead to improved promiscuity towards flavonoids including quercetin, luteolin, apigenin, morin, chrysin and naringenin Leuconostoc citreum
2.4.1.373 W2135F mutant displays 3-4fold improved activity with naringenin Leuconostoc mesenteroides
2.4.1.373 W2135G mutant displays 3-4fold improved activity with naringenin Leuconostoc mesenteroides
2.4.1.373 W2135G mutant synthesizes four times more morin glucoside than the wild-type Leuconostoc mesenteroides
2.4.1.373 W2135I/F2136Y mutant displays 3-4fold improved activity with naringenin, about 60% conversion. Mutant converts nearly 3-times more naringenin than luteolin (59% and 17%, conversion, respectively) Leuconostoc mesenteroides
2.4.1.373 W2135L-F2136L mutant significantly synthesizes quercetin di-glucoside Leuconostoc mesenteroides
2.4.1.373 W2135V mutant displays 3-4fold improved activity with naringenin Leuconostoc mesenteroides

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.B77 Leuconostoc citreum
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-
-
2.4.1.B77 Leuconostoc citreum NRRL B-1299
-
-
-
2.4.1.373 Leuconostoc mesenteroides Q8G9Q2
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-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.B77 additional information enzyme additionally has transglucosylation activity towards flavonoids including quercetin, luteolin, apigenin, morin, chrysin and naringenin Leuconostoc citreum ?
-
 ?
2.4.1.B77 additional information enzyme additionally has transglucosylation activity towards flavonoids including quercetin, luteolin, apigenin, morin, chrysin and naringenin Leuconostoc citreum NRRL B-1299 ?
-
 ?
2.4.1.373 sucrose + luteolin
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 Leuconostoc mesenteroides D-fructose + luteolin glucoside
-
 ?
2.4.1.373 sucrose + morin
-
 Leuconostoc mesenteroides D-fructose + morin glucoside one mono-glucoside, three di-glucosides and one tri-glucoside ?
2.4.1.373 sucrose + naringenin
-
 Leuconostoc mesenteroides D-fructose + naringenin glucoside one naringenin mono-glucoside, two di-glucosides and one tri-glucoside ?
2.4.1.373 sucrose + quercetin
-
 Leuconostoc mesenteroides D-fructose + quercetin glucoside
-
 ?