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Literature summary extracted from

  • Xia, B.; Liu, Y.; Guevara, J.; Li, J.; Jilich, C.; Yang, Y.; Wang, L.; Dominy, B.N.; Cao, W.
    Correlated mutation in the evolution of catalysis in uracil DNA glycosylase superfamily (2017), Sci. Rep., 7, 45978 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.27 expressed in Escherichia coli BL21(DE3) cells Thermus thermophilus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.2.27 E41A/G42D the mutant shows severely reduced activity compared to the wild type enzyme Thermus thermophilus
3.2.2.27 E41Q the mutant shows 888fold reduced activity compared to the wild type enzyme Thermus thermophilus
3.2.2.27 E41Q/G42D the mutant shows 5.3fold reduced activity compared to the wild type enzyme Thermus thermophilus
3.2.2.27 E47A the mutant shows severely reduced activity compared to the wild type enzyme Thermus thermophilus
3.2.2.27 F54A the mutant shows severely reduced activity compared to the wild type enzyme Thermus thermophilus
3.2.2.27 G42D the mutant shows 89fold reduced activity compared to the wild type enzyme Thermus thermophilus
3.2.2.27 H155S the mutant shows reduced activity compared to the wild type enzyme Thermus thermophilus
3.2.2.27 N80A the mutant shows severely reduced activity compared to the wild type enzyme Thermus thermophilus
3.2.2.27 N89A the mutant shows reduced activity compared to the wild type enzyme Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.27 0.00017
-
 double stranded DNA containing G-U mismatch mutant enzyme E41Q/G42D, at pH 7.6 and 60°C Thermus thermophilus
3.2.2.27 0.00074
-
 double stranded DNA containing G-U mismatch mutant enzyme N89A, at pH 7.6 and 60°C Thermus thermophilus
3.2.2.27 0.00076
-
 double stranded DNA containing G-U mismatch mutant enzyme H155S, at pH 7.6 and 60°C Thermus thermophilus
3.2.2.27 0.00097
-
 double stranded DNA containing G-U mismatch wild type enzyme, at pH 7.6 and 60°C Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.27 uracil-containing single-stranded DNA + H2O Thermus thermophilus
-
 uracil + single-stranded DNA with abasic site
-
 ?
3.2.2.27 uracil-mismatched double-stranded DNA + H2O Thermus thermophilus
-
 uracil + double-stranded DNA with abasic site
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.27 Thermus thermophilus Q5SKC5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.27
-
 Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.27 double stranded DNA containing G-U mismatch + H2O
-
 Thermus thermophilus uracil + double-stranded DNA with abasic site
-
 ?
3.2.2.27 additional information the enzyme does not show any detectable activity on other deaminated bases than uracil Thermus thermophilus ?
-
 ?
3.2.2.27 uracil-containing single-stranded DNA + H2O
-
 Thermus thermophilus uracil + single-stranded DNA with abasic site
-
 ?
3.2.2.27 uracil-mismatched double-stranded DNA + H2O
-
 Thermus thermophilus uracil + double-stranded DNA with abasic site
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.2.27 family 4 UDGa
-
 Thermus thermophilus
3.2.2.27 UDG
-
 Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.2.27 0.000072
-
 double stranded DNA containing G-U mismatch mutant enzyme H155S, at pH 7.6 and 60°C Thermus thermophilus
3.2.2.27 0.0075
-
 double stranded DNA containing G-U mismatch mutant enzyme E41Q/G42D, at pH 7.6 and 60°C Thermus thermophilus
3.2.2.27 0.025
-
 double stranded DNA containing G-U mismatch mutant enzyme N89A, at pH 7.6 and 60°C Thermus thermophilus
3.2.2.27 0.23
-
 double stranded DNA containing G-U mismatch wild type enzyme, at pH 7.6 and 60°C Thermus thermophilus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.2.27 0.0092
-
 double stranded DNA containing G-U mismatch mutant enzyme H155S, at pH 7.6 and 60°C Thermus thermophilus
3.2.2.27 35
-
 double stranded DNA containing G-U mismatch mutant enzyme N89A, at pH 7.6 and 60°C Thermus thermophilus
3.2.2.27 45
-
 double stranded DNA containing G-U mismatch mutant enzyme E41Q/G42D, at pH 7.6 and 60°C Thermus thermophilus
3.2.2.27 240
-
 double stranded DNA containing G-U mismatch wild type enzyme, at pH 7.6 and 60°C Thermus thermophilus