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Literature summary extracted from

  • Patil, K.; Mahajan, R.; Lautre, H.; Patil, V.
    Isolation, purification and characterization of acid phosphatase from Scenedesmus obliquus (2016), Res. J. Pharm. Biol. Chem. Sci., 7, 1584-1597 .
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.2 Ca2+ about 112% activity at 1 mM Tetradesmus obliquus
3.1.3.2 Cu2+ about 130% activity at 1 mM Tetradesmus obliquus
3.1.3.2 EDTA about 120% activity at 1 mM Tetradesmus obliquus
3.1.3.2 Fe3+ about 120% activity at 1 mM Tetradesmus obliquus
3.1.3.2 guaiacol about 120% activity at 1 mM Tetradesmus obliquus
3.1.3.2 Triton X-100 about 115% activity at 1 mM Tetradesmus obliquus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 acetone about 70% residual activity at 1 mM Tetradesmus obliquus
3.1.3.2 butanol about 40% residual activity at 1 mM Tetradesmus obliquus
3.1.3.2 chloroform about 30% residual activity at 1 mM Tetradesmus obliquus
3.1.3.2 hexane about 90% residual activity at 1 mM Tetradesmus obliquus
3.1.3.2 Hg2+ about 95% residual activity at 1 mM Tetradesmus obliquus
3.1.3.2 isoamyl alcohol about 80% residual activity at 1 mM Tetradesmus obliquus
3.1.3.2 K+ strong inhibition, about 55% residual activity at 1 mM Tetradesmus obliquus
3.1.3.2 Mg2+ about 95% residual activity at 1 mM Tetradesmus obliquus
3.1.3.2 SDS strong inhibition, about 10% residual activity at 1 mM Tetradesmus obliquus
3.1.3.2 Tween 80 strong inhibition, about 60% residual activity at 1 mM Tetradesmus obliquus
3.1.3.2 Zn2+ about 70% residual activity at 1 mM Tetradesmus obliquus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 0.24
-
 4-nitrophenyl phosphate at pH 5.0 and 37°C Tetradesmus obliquus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.2 56100
-
 liquid crystal mass spectrometry analysis Tetradesmus obliquus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Tetradesmus obliquus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2 ammonium sulfate precipitation, DEAE-cellulose column chromatography, and Sephadex G-75 gel filtration Tetradesmus obliquus

Storage Stability

EC Number Storage Stability Organism
3.1.3.2 4°C, enzyme extracted with ammonium sulfate precipitation, 1 week, 13.8% loss of activity Tetradesmus obliquus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 4-nitrophenyl phosphate + H2O
-
 Tetradesmus obliquus 4-nitrophenol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.2 monomer 1 * 59410, SDS-PAGE Tetradesmus obliquus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.2 50
-
-
 Tetradesmus obliquus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.2 30 60 the enzyme is most active between 30°C and 60°C. It retains more than 44% activity till 60°C, while the activity drops rapidly below 30°C and above 60°C Tetradesmus obliquus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.2 37 50 the enzyme is fairly stable for 120 min at temperature up to 37°C. Above 50°C, the activity declines rapidly as the temperature is increased Tetradesmus obliquus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 5
-
-
 Tetradesmus obliquus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.2 4 6 the enzyme shows the most activity in the pH range 4.0-6.0, and its activity decreases sharply above pH 5.0 retaining 12% of maximum activity Tetradesmus obliquus