BRENDA - Enzyme Database

Pichia pastoris engineering for the production of a modified phospholipase C

Elena, C.; Ravasi, P.; Cerminati, S.; Peiru, S.; Castelli, M.; Menzella, H.; Process Biochem. 51, 1935-1944 (2016)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.1.4.3
expressed in Pichia pastoris strain GS115
Bacillus cereus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.3
phosphatidylcholine + H2O
Bacillus cereus
preferred substrate
1,2-diacyl-sn-glycerol + phosphorylcholine
-
-
?
3.1.4.3
phosphatidylethanolamine + H2O
Bacillus cereus
-
1,2-sn-diacylglycerol + phosphorethanolamine
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.3
Bacillus cereus
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.3
4-nitrophenylphosphorylcholine + H2O
-
752075
Bacillus cereus
4-nitrophenol + phosphorylcholine
-
-
-
?
3.1.4.3
phosphatidylcholine + H2O
preferred substrate
752075
Bacillus cereus
1,2-diacyl-sn-glycerol + phosphorylcholine
-
-
-
?
3.1.4.3
phosphatidylethanolamine + H2O
-
752075
Bacillus cereus
1,2-sn-diacylglycerol + phosphorethanolamine
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.4.3
?
x * 30000, SDS-PAGE
Bacillus cereus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.3
expressed in Pichia pastoris strain GS115
Bacillus cereus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.3
phosphatidylcholine + H2O
Bacillus cereus
preferred substrate
1,2-diacyl-sn-glycerol + phosphorylcholine
-
-
?
3.1.4.3
phosphatidylethanolamine + H2O
Bacillus cereus
-
1,2-sn-diacylglycerol + phosphorethanolamine
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.3
4-nitrophenylphosphorylcholine + H2O
-
752075
Bacillus cereus
4-nitrophenol + phosphorylcholine
-
-
-
?
3.1.4.3
phosphatidylcholine + H2O
preferred substrate
752075
Bacillus cereus
1,2-diacyl-sn-glycerol + phosphorylcholine
-
-
-
?
3.1.4.3
phosphatidylethanolamine + H2O
-
752075
Bacillus cereus
1,2-sn-diacylglycerol + phosphorethanolamine
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.4.3
?
x * 30000, SDS-PAGE
Bacillus cereus