EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.B1 | expressed in Escherichia coli BL21-Star(DE3) cells | Agarivorans albus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.B1 | Al3+ | strong inhibition | Agarivorans albus | |
3.2.1.B1 | Cu2+ | strong inhibition | Agarivorans albus | |
3.2.1.B1 | Mn2+ | strong inhibition | Agarivorans albus | |
3.2.1.B1 | additional information | not influenced by EDTA, 2-mercaptoethanol, dithiothreitol, and urea | Agarivorans albus | |
3.2.1.B1 | Zn2+ | strong inhibition | Agarivorans albus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.B1 | 0.02 | - |
agarose | at pH 7.0 and 25°C | Agarivorans albus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.B1 | extracellular | - |
Agarivorans albus | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.B1 | additional information | neither significant activation nor inhibition in the presence of Na+, K+, Ca2+, Ba2+, and Mg2+ | Agarivorans albus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.B1 | agarose + H2O | Agarivorans albus | 100% activity | neoagarohexaose + neoagarotetraose + D-galactose | - |
? | |
3.2.1.B1 | carboxymethyl cellulose + H2O | Agarivorans albus | 1.4% activity compared to agarose | ? | - |
? | |
3.2.1.B1 | starch + H2O | Agarivorans albus | 1.8% activity compared to agarose | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.B1 | Agarivorans albus | A8W969 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.B1 | ammonium sulfate precipitation and DEAE Sepharose column chromatography | Agarivorans albus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.B1 | 29.48 | - |
crude extract, at pH 7.0 and 25°C | Agarivorans albus |
3.2.1.B1 | 265.32 | - |
after 9fold purification, at pH 7.0 and 25°C | Agarivorans albus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.B1 | agarose + H2O | 100% activity | Agarivorans albus | neoagarohexaose + neoagarotetraose + D-galactose | - |
? | |
3.2.1.B1 | carboxymethyl cellulose + H2O | 1.4% activity compared to agarose | Agarivorans albus | ? | - |
? | |
3.2.1.B1 | starch + H2O | 1.8% activity compared to agarose | Agarivorans albus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.B1 | ? | x * 45000, SDS-PAGE | Agarivorans albus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.B1 | beta-agarase | - |
Agarivorans albus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.B1 | 40 | - |
- |
Agarivorans albus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.B1 | 30 | 50 | the enzyme retains more than 80% of its maximal activity within a temperature range of 30°C to 50°C | Agarivorans albus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.B1 | 35 | 50 | the enzyme is thermostable at temperatures from 35-50°C with more than 80% activity remaining after 30 min incubation | Agarivorans albus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.B1 | 45 | - |
agarose | at pH 7.0 and 25°C | Agarivorans albus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.B1 | 7 | - |
- |
Agarivorans albus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.B1 | 5 | 9 | the enzyme retains more than 70% of its maximal activity within a pH range of 5.0-9.0 | Agarivorans albus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.B1 | 5 | 9 | the enzyme exhibits more than 80% of its maximal activity over a pH range of 5.0 to 9.0 after 1 h incubation and shows more than 40% of maximal activity at pH values below 4.0 and above 11.0 | Agarivorans albus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.B1 | 2250 | - |
agarose | at pH 7.0 and 25°C | Agarivorans albus |