Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yoon, S.Y.; Lee, H.M.; Kong, J.N.; Kong, K.H.
    Secretory expression and enzymatic characterization of recombinant Agarivorans albus beta-agarase in Escherichia coli (2017), Prep. Biochem. Biotechnol., 47, 1037-1042 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.B1 expressed in Escherichia coli BL21-Star(DE3) cells Agarivorans albus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.B1 Al3+ strong inhibition Agarivorans albus
3.2.1.B1 Cu2+ strong inhibition Agarivorans albus
3.2.1.B1 Mn2+ strong inhibition Agarivorans albus
3.2.1.B1 additional information not influenced by EDTA, 2-mercaptoethanol, dithiothreitol, and urea Agarivorans albus
3.2.1.B1 Zn2+ strong inhibition Agarivorans albus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.B1 0.02
-
agarose at pH 7.0 and 25°C Agarivorans albus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.B1 extracellular
-
Agarivorans albus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.B1 additional information neither significant activation nor inhibition in the presence of Na+, K+, Ca2+, Ba2+, and Mg2+ Agarivorans albus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.B1 agarose + H2O Agarivorans albus 100% activity neoagarohexaose + neoagarotetraose + D-galactose
-
?
3.2.1.B1 carboxymethyl cellulose + H2O Agarivorans albus 1.4% activity compared to agarose ?
-
?
3.2.1.B1 starch + H2O Agarivorans albus 1.8% activity compared to agarose ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.B1 Agarivorans albus A8W969
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.B1 ammonium sulfate precipitation and DEAE Sepharose column chromatography Agarivorans albus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.B1 29.48
-
crude extract, at pH 7.0 and 25°C Agarivorans albus
3.2.1.B1 265.32
-
after 9fold purification, at pH 7.0 and 25°C Agarivorans albus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.B1 agarose + H2O 100% activity Agarivorans albus neoagarohexaose + neoagarotetraose + D-galactose
-
?
3.2.1.B1 carboxymethyl cellulose + H2O 1.4% activity compared to agarose Agarivorans albus ?
-
?
3.2.1.B1 starch + H2O 1.8% activity compared to agarose Agarivorans albus ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.B1 ? x * 45000, SDS-PAGE Agarivorans albus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.B1 beta-agarase
-
Agarivorans albus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.B1 40
-
-
Agarivorans albus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.B1 30 50 the enzyme retains more than 80% of its maximal activity within a temperature range of 30°C to 50°C Agarivorans albus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.B1 35 50 the enzyme is thermostable at temperatures from 35-50°C with more than 80% activity remaining after 30 min incubation Agarivorans albus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.B1 45
-
agarose at pH 7.0 and 25°C Agarivorans albus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.B1 7
-
-
Agarivorans albus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.B1 5 9 the enzyme retains more than 70% of its maximal activity within a pH range of 5.0-9.0 Agarivorans albus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.B1 5 9 the enzyme exhibits more than 80% of its maximal activity over a pH range of 5.0 to 9.0 after 1 h incubation and shows more than 40% of maximal activity at pH values below 4.0 and above 11.0 Agarivorans albus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.B1 2250
-
agarose at pH 7.0 and 25°C Agarivorans albus