EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.18 | expressed in Escherichia coli Rosetta2(DE3)pLysS cells | Arabidopsis thaliana |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.18 | 0.0304 | - |
acrolein | at pH 6.0 and 25°C | Arabidopsis thaliana | |
2.5.1.18 | 0.0512 | - |
glutathione | at pH 6.0 and 25°C | Arabidopsis thaliana | |
2.5.1.18 | 0.0709 | - |
glutathione | at pH 6.0 and 25°C | Spinacia oleracea | |
2.5.1.18 | 0.093 | - |
acrolein | at pH 6.0 and 25°C | Spinacia oleracea | |
2.5.1.18 | 0.395 | - |
4-hydroxy-(E)-2-nonenal | at pH 6.0 and 25°C | Spinacia oleracea |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.18 | Arabidopsis thaliana | Q9ZRW8 | ecotype Columbia-0 | - |
2.5.1.18 | Spinacia oleracea | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.18 | GSTrap 4B column chromatography, HiTrap Q column chromatography, and DEAE-Sepharose column chromatography | Spinacia oleracea |
2.5.1.18 | GSTrap 4B column chromatography, HiTrap Q column chromatography, and DEAE-Sepharose column chromatography | Arabidopsis thaliana |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.5.1.18 | leaf | - |
Spinacia oleracea | - |
2.5.1.18 | leaf | - |
Arabidopsis thaliana | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.5.1.18 | 0.01 | - |
less than 0.01 micromol/min/mg with 0.1 mM 4-nitrobenzyl chloride as substrate, at pH 6.0 and 25°C | Spinacia oleracea |
2.5.1.18 | 0.01 | - |
less than 0.01 micromol/min/mg with 0.1 mM benzyl isothiocyanate as substrate, at pH 6.0 and 25°C | Spinacia oleracea |
2.5.1.18 | 0.01 | - |
less than 0.01 micromol/min/mg with 0.1 mM crotonaldehyde as substrate, at pH 6.0 and 25°C | Spinacia oleracea |
2.5.1.18 | 0.05 | - |
purified protein with poly(His) tag, with 12-oxo-phytodienoic acid as substrate, at pH 6.0 and 25°C | Arabidopsis thaliana |
2.5.1.18 | 0.07 | - |
with 0.1 mM 4-hydroxy-(E)-2-nonenal as substrate, at pH 6.0 and 25°C | Spinacia oleracea |
2.5.1.18 | 1.7 | - |
with 0.1 mM acrolein as substrate, at pH 6.0 and 25°C | Spinacia oleracea |
2.5.1.18 | 3.83 | - |
purified protein with poly(His) tag, with acrolein as substrate, at pH 6.0 and 25°C | Arabidopsis thaliana |
2.5.1.18 | 5.2 | - |
with 0.1 mM 1-chloro-2,4-dinitrobenzene as substrate, at pH 6.0 and 25°C | Spinacia oleracea |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.18 | 1-chloro-2,4-dinitrobenzene + glutathione | - |
Spinacia oleracea | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? | |
2.5.1.18 | 12-oxo-phytodienoic acid + glutathione | - |
Arabidopsis thaliana | ? | - |
? | |
2.5.1.18 | 4-hydroxy-(E)-2-nonenal + glutathione | - |
Spinacia oleracea | ? | - |
? | |
2.5.1.18 | 4-nitrobenzyl chloride + glutathione | - |
Spinacia oleracea | S-(4-nitrobenzyl)glutathione + HCl | - |
? | |
2.5.1.18 | acrolein + glutathione | - |
Spinacia oleracea | ? | - |
? | |
2.5.1.18 | acrolein + glutathione | - |
Arabidopsis thaliana | ? | - |
? | |
2.5.1.18 | benzyl isothiocyanate + glutathione | - |
Spinacia oleracea | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.18 | ? | x * 24000, SDS-PAGE | Spinacia oleracea |
2.5.1.18 | ? | x * 29000, SDS-PAGE | Arabidopsis thaliana |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.18 | GST | - |
Spinacia oleracea |
2.5.1.18 | GST Tau19 | - |
Arabidopsis thaliana |
2.5.1.18 | GST-Acr | isozyme | Spinacia oleracea |
2.5.1.18 | GSTU19 | - |
Arabidopsis thaliana |
2.5.1.18 | RX:glutathione R-transferase | - |
Spinacia oleracea |
2.5.1.18 | RX:glutathione R-transferase | - |
Arabidopsis thaliana |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.18 | 0.0709 | - |
glutathione | at pH 6.0 and 25°C | Spinacia oleracea | |
2.5.1.18 | 0.262 | - |
4-hydroxy-(E)-2-nonenal | at pH 6.0 and 25°C | Spinacia oleracea | |
2.5.1.18 | 1.15 | - |
acrolein | at pH 6.0 and 25°C | Spinacia oleracea | |
2.5.1.18 | 1.78 | - |
acrolein | at pH 6.0 and 25°C | Arabidopsis thaliana |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
2.5.1.18 | Spinacia oleracea | isoelectric focusing | - |
5.9 |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.5.1.18 | metabolism | the enzyme keeps the acrolein level low in plant cells | Spinacia oleracea |
2.5.1.18 | metabolism | the enzyme keeps the acrolein level low in plant cells | Arabidopsis thaliana |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.18 | 0.0709 | - |
glutathione | at pH 6.0 and 25°C | Spinacia oleracea | |
2.5.1.18 | 0.664 | - |
4-hydroxy-(E)-2-nonenal | at pH 6.0 and 25°C | Spinacia oleracea | |
2.5.1.18 | 12.3 | - |
acrolein | at pH 6.0 and 25°C | Spinacia oleracea | |
2.5.1.18 | 59.3 | - |
acrolein | at pH 6.0 and 25°C | Arabidopsis thaliana |