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Literature summary extracted from

  • Vialart, G.; Hehn, A.; Olry, A.; Ito, K.; Krieger, C.; Larbat, R.; Paris, C.; Shimizu, B.; Sugimoto, Y.; Mizutani, M.; Bourgaud, F.
    A 2-oxoglutarate-dependent dioxygenase from Ruta graveolens L. Exhibits p-coumaroyl CoA 2-hydroxylase activity (C2H) A missing step in the synthesis of umbelliferone in plants (2012), Plant J., 70, 460-470 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.61 expression in Escherichia coli and Nicotiana benthamiana Ruta graveolens
1.14.11.62 expression in Escherichia coli and Nicotiana benthamiana Ruta graveolens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.61 psoralen
-
 Ruta graveolens
1.14.11.62 psoralen
-
 Ruta graveolens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.61 0.037
-
 trans-feruloyl-CoA pH 7.2, 30°C Ruta graveolens
1.14.11.62 0.05
-
 trans-feruloyl-CoA pH 7.2, 30°C Ruta graveolens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.62 additional information Ruta graveolens no substrates: cinnamic acid, caffeic acid, p-coumaric acid, ferulic acid, cinnamoyl-CoA, caffeoyl-CoA ?
-
 ?
1.14.11.62 trans-4-coumaroyl-CoA + 2-oxoglutarate + O2 Ruta graveolens
-
 2,4-dihydroxycinnamoyl-CoA + succinate + CO2
-
 ?
1.14.11.62 trans-feruloyl-CoA + 2-oxoglutarate + O2 Ruta graveolens reaction of EC 1.14.11.61 trans-6-hydroxyferuloyl-CoA + succinate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.61 Ruta graveolens H9LJF9 bifunctional enzyme, catalyzes the activities of EC 1.14.11.61 and EC 1.14.11.62 with equal activities
-
1.14.11.62 Ruta graveolens H9LJF9 bifunctional enzyme, catalyzes the activities of EC 1.14.11.61 and EC 1.14.11.62 with equal activities
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.61 leaf expression is induced by UV light Ruta graveolens
-
1.14.11.61 petiole expression is induced by UV light Ruta graveolens
-
1.14.11.61 root
-
 Ruta graveolens
-
1.14.11.61 stem
-
 Ruta graveolens
-
1.14.11.62 leaf expression is induced by UV light Ruta graveolens
-
1.14.11.62 petiole expression is induced by UV light Ruta graveolens
-
1.14.11.62 root
-
 Ruta graveolens
-
1.14.11.62 stem
-
 Ruta graveolens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.61 additional information no substrates: cinnamic acid, caffeic acid, p-coumaric acid, ferulic acid, cinnamoyl-CoA, caffeoyl-CoA Ruta graveolens ?
-
 ?
1.14.11.61 trans-4-coumaroyl-CoA + 2-oxoglutarate + O2
-
 Ruta graveolens 2,4-dihydroxycinnamoyl-CoA + succinate + CO2
-
 ?
1.14.11.61 trans-feruloyl-CoA + 2-oxoglutarate + O2
-
 Ruta graveolens trans-6-hydroxyferuloyl-CoA + succinate + CO2
-
 ?
1.14.11.62 additional information no substrates: cinnamic acid, caffeic acid, p-coumaric acid, ferulic acid, cinnamoyl-CoA, caffeoyl-CoA Ruta graveolens ?
-
 ?
1.14.11.62 trans-4-coumaroyl-CoA + 2-oxoglutarate + O2
-
 Ruta graveolens 2,4-dihydroxycinnamoyl-CoA + succinate + CO2
-
 ?
1.14.11.62 trans-feruloyl-CoA + 2-oxoglutarate + O2 reaction of EC 1.14.11.61 Ruta graveolens trans-6-hydroxyferuloyl-CoA + succinate + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.11.61 CYP98A22
-
 Ruta graveolens
1.14.11.62 CYP98A22
-
 Ruta graveolens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.11.61 0.122
-
 psoralen pH 7.2, 30°C Ruta graveolens
1.14.11.62 0.111
-
 psoralen pH 7.2, 30°C Ruta graveolens

Expression

EC Number Organism Comment Expression
1.14.11.61 Ruta graveolens in leaf and petiole, expression is induced by UV light up
1.14.11.62 Ruta graveolens in leaf and petiole, expression is induced by UV light up

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14.11.61 0.013
-
 trans-feruloyl-CoA pH 7.2, 30°C Ruta graveolens
1.14.11.62 0.014
-
 trans-feruloyl-CoA pH 7.2, 30°C Ruta graveolens