EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.3.8 | gene PAPhy, recombinant expression in transgenic Hordeum vulgare plants using transfection via Agrobacterium tumefaciens strain AGL0, T1-plants are transformed with 35S:PAPhy_a showing mature grain phytase activity, in green leaves and mature dry straw, phytase activities are increased significantly by 110fold (52000 FTU/kg) and 57fold (51000 FTU/kg), respectively | Hordeum vulgare |
3.1.3.26 | gene PAPhy, recombinant expression in transgenic Hordeum vulgare plants using transfection via Agrobacterium tumefaciens strain AGL0, T1-plants are transformed with 35S:PAPhy_a showing mature grain phytase activity, in green leaves and mature dry straw, phytase activities are increased significantly by 110fold (52000 FTU/kg) and 57fold (51000 FTU/kg), respectively | Hordeum vulgare |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.3.8 | additional information | the HvPAPhy_a-transformed barley plants with high phytase activities possess triple potential utilities for the improvement of phosphate bioavailability. First of all, the utilization of the mature grains as feed to increase the release of bio-available phosphate and minerals bound to the phytate of the grains, secondly, the utilization of the powdered straw either directly or phytase extracted hereof as a supplement to high phytate feed or food, and finally, the use of the stubble to be ploughed into the soil for mobilizing phytatebound phosphate for plant growth | Hordeum vulgare |
3.1.3.26 | additional information | the HvPAPhy_a-transformed barley plants with high phytase activities possess triple potential utilities for the improvement of phosphate bioavailability. First of all, the utilization of the mature grains as feed to increase the release of bio-available phosphate and minerals bound to the phytate of the grains, secondly, the utilization of the powdered straw either directly or phytase extracted hereof as a supplement to high phytate feed or food, and finally, the use of the stubble to be ploughed into the soil for mobilizing phytatebound phosphate for plant growth | Hordeum vulgare |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.8 | additional information | phytase enzymes in general possess a native resistance towards proteolytic degradation | Hordeum vulgare | |
3.1.3.26 | additional information | phytase enzymes in general possess a native resistance towards proteolytic degradation | Hordeum vulgare |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.8 | myo-inositol hexakisphosphate + H2O | Hordeum vulgare | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 | 1D-myo-inositol pentakisphosphate + phosphate | - |
? | |
3.1.3.26 | myo-inositol hexakisphosphate + H2O | Hordeum vulgare | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 | 1D-myo-inositol pentakisphosphate + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.8 | Hordeum vulgare | C4PKL2 | - |
- |
3.1.3.26 | Hordeum vulgare | C4PKL2 | - |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.1.3.8 | additional information | phytase enzymes in general possess a native resistance towards proteolytic degradation | Hordeum vulgare |
3.1.3.26 | additional information | phytase enzymes in general possess a native resistance towards proteolytic degradation | Hordeum vulgare |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.3.8 | awn | - |
Hordeum vulgare | - |
3.1.3.8 | chaff | - |
Hordeum vulgare | - |
3.1.3.8 | leaf | green flag leaves of T2-plants | Hordeum vulgare | - |
3.1.3.8 | rachis | - |
Hordeum vulgare | - |
3.1.3.8 | seed | grain, the enzyme is expressed during barley seed development | Hordeum vulgare | - |
3.1.3.8 | stem | - |
Hordeum vulgare | - |
3.1.3.8 | whole plant | barley plants constitutively express HvPAPhy_a, high preformed phytase levels in the mature dry vegetative material of the 35S:PAPhy_a-plant. Expression analysis in different plant tissues, overview. Positive correlation between phytase activity and plants being heterozygous or homozygous for the HvPAPhy_a cisgene (the genomic clone of HvPAPhy_a including the native promoter and terminator of the gene) | Hordeum vulgare | - |
3.1.3.26 | awn | - |
Hordeum vulgare | - |
3.1.3.26 | chaff | - |
Hordeum vulgare | - |
3.1.3.26 | leaf | green flag leaves of T2-plants | Hordeum vulgare | - |
3.1.3.26 | rachis | - |
Hordeum vulgare | - |
3.1.3.26 | seed | grain, the enzyme is expressed during barley seed development | Hordeum vulgare | - |
3.1.3.26 | stem | - |
Hordeum vulgare | - |
3.1.3.26 | whole plant | barley plants constitutively express HvPAPhy_a, high preformed phytase levels in the mature dry vegetative material of the 35S:PAPhy_a-plant. Expression analysis in different plant tissues, overview. Positive correlation between phytase activity and plants being heterozygous or homozygous for the HvPAPhy_a cisgene (the genomic clone of HvPAPhy_a including the native promoter and terminator of the gene) | Hordeum vulgare | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.8 | additional information | the enzyme also shows phosphate-monoester phosphohydrolase activity, EC 3.1.3.2 | Hordeum vulgare | ? | - |
? | |
3.1.3.8 | myo-inositol hexakisphosphate + H2O | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 | Hordeum vulgare | 1D-myo-inositol pentakisphosphate + phosphate | - |
? | |
3.1.3.26 | additional information | the enzyme also shows phosphate-monoester phosphohydrolase activity, EC 3.1.3.2 | Hordeum vulgare | ? | - |
? | |
3.1.3.26 | myo-inositol hexakisphosphate + H2O | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 | Hordeum vulgare | 1D-myo-inositol pentakisphosphate + phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.3.8 | HvPAPhy_a | - |
Hordeum vulgare |
3.1.3.8 | More | cf. EC 3.1.3.2 and 3.1.3.26 | Hordeum vulgare |
3.1.3.8 | PAPhy | - |
Hordeum vulgare |
3.1.3.8 | phytase | - |
Hordeum vulgare |
3.1.3.8 | purple acid phosphatase | - |
Hordeum vulgare |
3.1.3.26 | HvPAPhy_a | - |
Hordeum vulgare |
3.1.3.26 | More | cf. EC 3.1.3.2 and 3.1.3.8 | Hordeum vulgare |
3.1.3.26 | PAPhy | - |
Hordeum vulgare |
3.1.3.26 | phytase | - |
Hordeum vulgare |
3.1.3.26 | purple acid phosphatase | - |
Hordeum vulgare |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 55 | - |
- |
Hordeum vulgare |
3.1.3.26 | 55 | - |
- |
Hordeum vulgare |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 5.5 | - |
- |
Hordeum vulgare |
3.1.3.26 | 5.5 | - |
- |
Hordeum vulgare |