BRENDA - Enzyme Database

Purification, sequencing and characterization of phospholipase D from Indian mustard seeds

Khatoon, H.; Mansfeld, J.; Schierhorn, A.; Ulbrich-Hofmann, R.; Phytochemistry 117, 65-75 (2015)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.4
phosphatidylinositol 4,5-bisphosphate
strong activating effect at 2-10 mM CaCl2
Brassica juncea var. juncea
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.4
Na3VO4
-
Brassica juncea var. juncea
3.1.4.4
NaAlF4
-
Brassica juncea var. juncea
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.4
1.84
-
phosphatidylcholine
pH 5.5, 37°C
Brassica juncea var. juncea
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.4
Ca2+
maximum activity above 60 mM
Brassica juncea var. juncea
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.4
Brassica juncea var. juncea
A0A0G4AKV1
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.4
43.6
-
substrate phosphatidylglycerol, pH 5.5, 37°C
Brassica juncea var. juncea
3.1.4.4
117.8
-
substrate phosphatidylethanolamine, pH 5.5, 37°C
Brassica juncea var. juncea
3.1.4.4
253.8
-
substrate phosphatidylcholine, pH 5.5, 37°C
Brassica juncea var. juncea
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
additional information
phosphatidylcholines with short-chain fatty acids are better substrates than phosphatidylcholines with long fatty acid chains. Lysophosphatidylcholine is not accepted as substrate
751819
Brassica juncea var. juncea
?
-
-
-
-
3.1.4.4
phosphatidylcholine + H2O
-
751819
Brassica juncea var. juncea
choline + phosphatidate
-
-
-
?
3.1.4.4
phosphatidylethanolamine + H2O
-
751819
Brassica juncea var. juncea
ethanolamine + phosphatidate
-
-
-
?
3.1.4.4
phosphatidylglycerol + H2O
-
751819
Brassica juncea var. juncea
glycerol + phosphatidate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.4.4
?
x * 92000, SDS-PAGE and calculated from sequence
Brassica juncea var. juncea
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.4.4
45
-
inactivation above
Brassica juncea var. juncea
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.4
5.5
6
-
Brassica juncea var. juncea
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.1.4.4
Brassica juncea var. juncea
calculated from sequence
-
5.4
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
3.1.4.4
0.0078
-
pH 5.5, 37°C
Brassica juncea var. juncea
NaAlF4
3.1.4.4
0.033
-
pH 5.5, 37°C
Brassica juncea var. juncea
Na3VO4
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.4
phosphatidylinositol 4,5-bisphosphate
strong activating effect at 2-10 mM CaCl2
Brassica juncea var. juncea
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
3.1.4.4
0.0078
-
pH 5.5, 37°C
Brassica juncea var. juncea
NaAlF4
3.1.4.4
0.033
-
pH 5.5, 37°C
Brassica juncea var. juncea
Na3VO4
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.4
Na3VO4
-
Brassica juncea var. juncea
3.1.4.4
NaAlF4
-
Brassica juncea var. juncea
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.4
1.84
-
phosphatidylcholine
pH 5.5, 37°C
Brassica juncea var. juncea
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.4
Ca2+
maximum activity above 60 mM
Brassica juncea var. juncea
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.4
43.6
-
substrate phosphatidylglycerol, pH 5.5, 37°C
Brassica juncea var. juncea
3.1.4.4
117.8
-
substrate phosphatidylethanolamine, pH 5.5, 37°C
Brassica juncea var. juncea
3.1.4.4
253.8
-
substrate phosphatidylcholine, pH 5.5, 37°C
Brassica juncea var. juncea
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
additional information
phosphatidylcholines with short-chain fatty acids are better substrates than phosphatidylcholines with long fatty acid chains. Lysophosphatidylcholine is not accepted as substrate
751819
Brassica juncea var. juncea
?
-
-
-
-
3.1.4.4
phosphatidylcholine + H2O
-
751819
Brassica juncea var. juncea
choline + phosphatidate
-
-
-
?
3.1.4.4
phosphatidylethanolamine + H2O
-
751819
Brassica juncea var. juncea
ethanolamine + phosphatidate
-
-
-
?
3.1.4.4
phosphatidylglycerol + H2O
-
751819
Brassica juncea var. juncea
glycerol + phosphatidate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.4.4
?
x * 92000, SDS-PAGE and calculated from sequence
Brassica juncea var. juncea
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.4.4
45
-
inactivation above
Brassica juncea var. juncea
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.4
5.5
6
-
Brassica juncea var. juncea
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.1.4.4
Brassica juncea var. juncea
calculated from sequence
-
5.4