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Literature summary extracted from

  • Ishino, S.; Nishi, Y.; Oda, S.; Uemori, T.; Sagara, T.; Takatsu, N.; Yamagami, T.; Shirai, T.; Ishino, Y.
    Identification of a mismatch-specific endonuclease in hyperthermophilic Archaea (2016), Nucleic Acids Res., 44, 2977-2986 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.1.21.B3 molecular biology the thermostable mismatch-specific endonuclease will be applicable to genetic engineering techniques Thermococcus kodakarensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.21.B3 expression in Escherichia coli BL21 Thermococcus kodakarensis

General Stability

EC Number General Stability Organism
3.1.21.B3 activity is distinctly salt-resistant in vitro Thermococcus kodakarensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.21.B3 28595
-
monomer, calculated from sequence Thermococcus kodakarensis
3.1.21.B3 60200
-
gel filtration Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.21.B3 DNA + H2O Thermococcus kodakarensis by cleavage of the double-strand the enzyme initiates a mismatch repair process. The enzyme specifically cleaves both strands of double-stranded DNA into 5'-protruding forms, with the mismatched base pair in the central position. EndoMS cleaves G/T, G/G, T/T, T/C and A/G mismatches, with preference for G/T, G/G and T/T. It has very little or no effect on C/C, A/C and A/A mismatches ?
-
?
3.1.21.B3 DNA + H2O Thermococcus kodakarensis ATCC BAA-918 by cleavage of the double-strand the enzyme initiates a mismatch repair process. The enzyme specifically cleaves both strands of double-stranded DNA into 5'-protruding forms, with the mismatched base pair in the central position. EndoMS cleaves G/T, G/G, T/T, T/C and A/G mismatches, with preference for G/T, G/G and T/T. It has very little or no effect on C/C, A/C and A/A mismatches ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.B3 Thermococcus kodakarensis Q5JER9
-
-
3.1.21.B3 Thermococcus kodakarensis ATCC BAA-918 Q5JER9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.21.B3
-
Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.B3 DNA + H2O by cleavage of the double-strand the enzyme initiates a mismatch repair process. The enzyme specifically cleaves both strands of double-stranded DNA into 5'-protruding forms, with the mismatched base pair in the central position. EndoMS cleaves G/T, G/G, T/T, T/C and A/G mismatches, with preference for G/T, G/G and T/T. It has very little or no effect on C/C, A/C and A/A mismatches Thermococcus kodakarensis ?
-
?
3.1.21.B3 DNA + H2O the enzyme specifically cleaves both strands of double-stranded DNA into 5'-protruding forms, with the mismatched base pair in the central position. EndoMS cleaves G/T, G/G, T/T, T/C and A/G mismatches, with more preference for G/T, G/G and T/T. It has very little or no effect on C/C, A/C and A/A mismatches Thermococcus kodakarensis ?
-
?
3.1.21.B3 DNA + H2O by cleavage of the double-strand the enzyme initiates a mismatch repair process. The enzyme specifically cleaves both strands of double-stranded DNA into 5'-protruding forms, with the mismatched base pair in the central position. EndoMS cleaves G/T, G/G, T/T, T/C and A/G mismatches, with preference for G/T, G/G and T/T. It has very little or no effect on C/C, A/C and A/A mismatches Thermococcus kodakarensis ATCC BAA-918 ?
-
?
3.1.21.B3 DNA + H2O the enzyme specifically cleaves both strands of double-stranded DNA into 5'-protruding forms, with the mismatched base pair in the central position. EndoMS cleaves G/T, G/G, T/T, T/C and A/G mismatches, with more preference for G/T, G/G and T/T. It has very little or no effect on C/C, A/C and A/A mismatches Thermococcus kodakarensis ATCC BAA-918 ?
-
?
3.1.21.B3 additional information dsDNA containing a one-base insertion/deletion is cleaved less efficiently than a mismatched DNA, and two and three-base insertions/deletions are not the target of the enzyme Thermococcus kodakarensis ?
-
?
3.1.21.B3 additional information dsDNA containing a one-base insertion/deletion is cleaved less efficiently than a mismatched DNA, and two and three-base insertions/deletions are not the target of the enzyme Thermococcus kodakarensis ATCC BAA-918 ?
-
?

Subunits

EC Number Subunits Comment Organism
3.1.21.B3 dimer 2 * 28595, calculated from sequence, SDS-PAGE Thermococcus kodakarensis

Synonyms

EC Number Synonyms Comment Organism
3.1.21.B3 endonuclease NucS
-
Thermococcus kodakarensis
3.1.21.B3 TK1898
-
Thermococcus kodakarensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.21.B3 60
-
the optimal temperature of the reaction is around 60°C Thermococcus kodakarensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.21.B3 80
-
80% of the activity remains after 30 min at 80°C Thermococcus kodakarensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.21.B3 6 11
-
Thermococcus kodakarensis

General Information

EC Number General Information Comment Organism
3.1.21.B3 physiological function the enzyme initiates a mismatch repair process Thermococcus kodakarensis