EC Number | Application | Comment | Organism |
---|---|---|---|
3.1.21.B3 | molecular biology | the thermostable mismatch-specific endonuclease will be applicable to genetic engineering techniques | Thermococcus kodakarensis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.21.B3 | expression in Escherichia coli BL21 | Thermococcus kodakarensis |
EC Number | General Stability | Organism |
---|---|---|
3.1.21.B3 | activity is distinctly salt-resistant in vitro | Thermococcus kodakarensis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.21.B3 | 28595 | - |
monomer, calculated from sequence | Thermococcus kodakarensis |
3.1.21.B3 | 60200 | - |
gel filtration | Thermococcus kodakarensis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.21.B3 | DNA + H2O | Thermococcus kodakarensis | by cleavage of the double-strand the enzyme initiates a mismatch repair process. The enzyme specifically cleaves both strands of double-stranded DNA into 5'-protruding forms, with the mismatched base pair in the central position. EndoMS cleaves G/T, G/G, T/T, T/C and A/G mismatches, with preference for G/T, G/G and T/T. It has very little or no effect on C/C, A/C and A/A mismatches | ? | - |
? | |
3.1.21.B3 | DNA + H2O | Thermococcus kodakarensis ATCC BAA-918 | by cleavage of the double-strand the enzyme initiates a mismatch repair process. The enzyme specifically cleaves both strands of double-stranded DNA into 5'-protruding forms, with the mismatched base pair in the central position. EndoMS cleaves G/T, G/G, T/T, T/C and A/G mismatches, with preference for G/T, G/G and T/T. It has very little or no effect on C/C, A/C and A/A mismatches | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.21.B3 | Thermococcus kodakarensis | Q5JER9 | - |
- |
3.1.21.B3 | Thermococcus kodakarensis ATCC BAA-918 | Q5JER9 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.21.B3 | - |
Thermococcus kodakarensis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.21.B3 | DNA + H2O | by cleavage of the double-strand the enzyme initiates a mismatch repair process. The enzyme specifically cleaves both strands of double-stranded DNA into 5'-protruding forms, with the mismatched base pair in the central position. EndoMS cleaves G/T, G/G, T/T, T/C and A/G mismatches, with preference for G/T, G/G and T/T. It has very little or no effect on C/C, A/C and A/A mismatches | Thermococcus kodakarensis | ? | - |
? | |
3.1.21.B3 | DNA + H2O | the enzyme specifically cleaves both strands of double-stranded DNA into 5'-protruding forms, with the mismatched base pair in the central position. EndoMS cleaves G/T, G/G, T/T, T/C and A/G mismatches, with more preference for G/T, G/G and T/T. It has very little or no effect on C/C, A/C and A/A mismatches | Thermococcus kodakarensis | ? | - |
? | |
3.1.21.B3 | DNA + H2O | by cleavage of the double-strand the enzyme initiates a mismatch repair process. The enzyme specifically cleaves both strands of double-stranded DNA into 5'-protruding forms, with the mismatched base pair in the central position. EndoMS cleaves G/T, G/G, T/T, T/C and A/G mismatches, with preference for G/T, G/G and T/T. It has very little or no effect on C/C, A/C and A/A mismatches | Thermococcus kodakarensis ATCC BAA-918 | ? | - |
? | |
3.1.21.B3 | DNA + H2O | the enzyme specifically cleaves both strands of double-stranded DNA into 5'-protruding forms, with the mismatched base pair in the central position. EndoMS cleaves G/T, G/G, T/T, T/C and A/G mismatches, with more preference for G/T, G/G and T/T. It has very little or no effect on C/C, A/C and A/A mismatches | Thermococcus kodakarensis ATCC BAA-918 | ? | - |
? | |
3.1.21.B3 | additional information | dsDNA containing a one-base insertion/deletion is cleaved less efficiently than a mismatched DNA, and two and three-base insertions/deletions are not the target of the enzyme | Thermococcus kodakarensis | ? | - |
? | |
3.1.21.B3 | additional information | dsDNA containing a one-base insertion/deletion is cleaved less efficiently than a mismatched DNA, and two and three-base insertions/deletions are not the target of the enzyme | Thermococcus kodakarensis ATCC BAA-918 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.21.B3 | dimer | 2 * 28595, calculated from sequence, SDS-PAGE | Thermococcus kodakarensis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.21.B3 | endonuclease NucS | - |
Thermococcus kodakarensis |
3.1.21.B3 | TK1898 | - |
Thermococcus kodakarensis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.21.B3 | 60 | - |
the optimal temperature of the reaction is around 60°C | Thermococcus kodakarensis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.21.B3 | 80 | - |
80% of the activity remains after 30 min at 80°C | Thermococcus kodakarensis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.21.B3 | 6 | 11 | - |
Thermococcus kodakarensis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.1.21.B3 | physiological function | the enzyme initiates a mismatch repair process | Thermococcus kodakarensis |