EC Number | Cloned (Comment) | Organism |
---|---|---|
1.2.1.103 | expressed in Escherichia coli | Thermus thermophilus |
3.5.1.130 | expression in Escherichia coli | Thermus thermophilus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.130 | [carrier protein LysW]-C-terminal-alpha-(L-lysyl)-L-glutamate | - |
Thermus thermophilus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.103 | Co2+ | 0.01 mM used in assay conditions | Thermus thermophilus | |
1.2.1.103 | Mg2+ | 0.01 mM used in assay conditions | Thermus thermophilus | |
1.2.1.103 | Zn2+ | 0.01 mM used in assay conditions | Thermus thermophilus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.103 | an [amino group carrier protein]-C-terminal-N-(1-carboxy-5-phosphooxy-5-oxopentan-1-yl)-L-glutamine + NADPH + H+ | Thermus thermophilus | - |
an [amino group carrier protein]-C-terminal-N-(1-carboxy-5-oxopentan-1-yl)-L-glutamine + phosphate + NADP+ | - |
r | |
2.7.2.17 | ATP + an [amino group carrier protein]-C-terminal-N-(1,4-dicarboxybutan-1-yl)-L-glutamine | Thermus thermophilus | the enzyme participates in an L-lysine biosynthetric pathway | ADP + phosphate + an [amino group carrier protein]-C-terminal-N-(1-carboxy-5-phosphooxy-5-oxopentan-1-yl)-L-glutamine | - |
? | |
3.5.1.130 | additional information | Thermus thermophilus | for the conversion of alpha-aminoadipate to lysine, the amino group of alpha-aminoadipate is modified by attachment to the gamma-carboxyl group of the C-terminal Glu54 of a small protein, LysW. The side chain of alpha-aminoadipate is converted to the lysyl side chain while still attached to LysW, and lysine is subsequently liberated from the LysW-lysine fusion | ? | - |
? | |
3.5.1.130 | additional information | Thermus thermophilus DSM 7039 | for the conversion of alpha-aminoadipate to lysine, the amino group of alpha-aminoadipate is modified by attachment to the gamma-carboxyl group of the C-terminal Glu54 of a small protein, LysW. The side chain of alpha-aminoadipate is converted to the lysyl side chain while still attached to LysW, and lysine is subsequently liberated from the LysW-lysine fusion | ? | - |
? | |
3.5.1.130 | [carrier protein LysW]-C-terminal-gamma-(L-lysyl)-L-glutamate + H2O | Thermus thermophilus | - |
[carrier protein LysW]-C-terminal-L-glutamate + L-lysine | - |
? | |
3.5.1.130 | [carrier protein LysW]-C-terminal-gamma-(L-lysyl)-L-glutamate + H2O | Thermus thermophilus DSM 7039 | - |
[carrier protein LysW]-C-terminal-L-glutamate + L-lysine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.103 | Thermus thermophilus | - |
- |
- |
2.6.1.118 | Thermus thermophilus | Q93R93 | - |
- |
2.7.2.17 | Thermus thermophilus | - |
- |
- |
3.5.1.130 | Thermus thermophilus | Q8VUS5 | - |
- |
3.5.1.130 | Thermus thermophilus DSM 7039 | Q8VUS5 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.1.130 | recombinant protein | Thermus thermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.103 | an [amino group carrier protein]-C-terminal-N-(1-carboxy-5-phosphooxy-5-oxopentan-1-yl)-L-glutamine + NADPH + H+ | - |
Thermus thermophilus | an [amino group carrier protein]-C-terminal-N-(1-carboxy-5-oxopentan-1-yl)-L-glutamine + phosphate + NADP+ | - |
r | |
2.6.1.118 | [carrier protein LysW]-C-terminal-N-(1-carboxy-5-oxopentan-1-yl)-L-glutamine + L-glutamate | - |
Thermus thermophilus | [carrier protein LysW]-C-terminal-gamma-(L-lysyl)-L-glutamate + 2-oxoglutarate | - |
? | |
2.7.2.17 | ATP + an [amino group carrier protein]-C-terminal-N-(1,4-dicarboxybutan-1-yl)-L-glutamine | - |
Thermus thermophilus | ADP + phosphate + an [amino group carrier protein]-C-terminal-N-(1-carboxy-5-phosphooxy-5-oxopentan-1-yl)-L-glutamine | - |
? | |
2.7.2.17 | ATP + an [amino group carrier protein]-C-terminal-N-(1,4-dicarboxybutan-1-yl)-L-glutamine | the enzyme participates in an L-lysine biosynthetric pathway | Thermus thermophilus | ADP + phosphate + an [amino group carrier protein]-C-terminal-N-(1-carboxy-5-phosphooxy-5-oxopentan-1-yl)-L-glutamine | - |
? | |
3.5.1.130 | additional information | for the conversion of alpha-aminoadipate to lysine, the amino group of alpha-aminoadipate is modified by attachment to the gamma-carboxyl group of the C-terminal Glu54 of a small protein, LysW. The side chain of alpha-aminoadipate is converted to the lysyl side chain while still attached to LysW, and lysine is subsequently liberated from the LysW-lysine fusion | Thermus thermophilus | ? | - |
? | |
3.5.1.130 | additional information | no substrates: [carrier protein LysW]-C-terminal-alpha-(L-lysyl)-L-glutamate, [carrier protein LysW]-13-alpha-(L-lysyl)-L-glutamate | Thermus thermophilus | ? | - |
? | |
3.5.1.130 | additional information | for the conversion of alpha-aminoadipate to lysine, the amino group of alpha-aminoadipate is modified by attachment to the gamma-carboxyl group of the C-terminal Glu54 of a small protein, LysW. The side chain of alpha-aminoadipate is converted to the lysyl side chain while still attached to LysW, and lysine is subsequently liberated from the LysW-lysine fusion | Thermus thermophilus DSM 7039 | ? | - |
? | |
3.5.1.130 | additional information | no substrates: [carrier protein LysW]-C-terminal-alpha-(L-lysyl)-L-glutamate, [carrier protein LysW]-13-alpha-(L-lysyl)-L-glutamate | Thermus thermophilus DSM 7039 | ? | - |
? | |
3.5.1.130 | [carrier protein LysW]-C-terminal-gamma-(L-lysyl)-L-glutamate + H2O | - |
Thermus thermophilus | [carrier protein LysW]-C-terminal-L-glutamate + L-lysine | - |
? | |
3.5.1.130 | [carrier protein LysW]-C-terminal-gamma-(L-lysyl)-L-glutamate + H2O | - |
Thermus thermophilus DSM 7039 | [carrier protein LysW]-C-terminal-L-glutamate + L-lysine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.1.103 | ? | x * about 40000, SDS-PAGE | Thermus thermophilus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.1.103 | lysY | - |
Thermus thermophilus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.103 | ATP | 20 mM used in assay conditions | Thermus thermophilus | |
1.2.1.103 | NADP+ | - |
Thermus thermophilus | |
1.2.1.103 | NADPH | - |
Thermus thermophilus | |
1.2.1.103 | pyridoxal 5'-phosphate | 5 mM used in assay conditions | Thermus thermophilus |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
3.5.1.130 | 0.00067 | - |
pH 8.0, 60°C | Thermus thermophilus | [carrier protein LysW]-C-terminal-alpha-(L-lysyl)-L-glutamate |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.6.1.118 | physiological function | for the conversion of alpha-aminoadipate to lysine, the amino group of alpha-aminoadipate is modified by attachment to the gamma-carboxyl group of the C-terminal Glu54 of a small protein, LysW. The side chain of alpha-aminoadipate is converted to the lysyl side chain while still attached to LysW, and lysine is subsequently liberated from the LysW-lysine fusion | Thermus thermophilus |
2.7.2.17 | physiological function | the enzyme participates in an L-lysine biosynthetic pathway | Thermus thermophilus |