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Literature summary extracted from

  • Sunner, H.; Charavgi, M.; Olsson, L.; Topakas, E.; Christakopoulos, P.
    Glucuronoyl esterase screening and characterization assays utilizing commercially available benzyl glucuronic acid ester (2015), Molecules, 20, 17807-17817 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.1.1.117 biofuel production broad range of industrial applications for the biocatalytic processes of lignocellulose degradation and modification of lignocellulosic materials Thermothelomyces thermophilus
3.1.1.117 biofuel production broad range of industrial applications for the biocatalytic processes of lignocellulose degradation and modification of lignocellulosic materials Trichoderma reesei
3.1.1.117 biofuel production broad range of industrial applications for the biocatalytic processes of lignocellulose degradation and modification of lignocellulosic materials Podospora anserina
3.1.1.117 biofuel production broad range of industrial applications for the biocatalytic processes of lignocellulose degradation and modification of lignocellulosic materials Schizophyllum commune
3.1.1.117 biofuel production broad range of industrial applications for the biocatalytic processes of lignocellulose degradation and modification of lignocellulosic materials Cerrena unicolor
3.1.1.117 biofuel production broad range of industrial applications for the biocatalytic processes of lignocellulose degradation and modification of lignocellulosic materials Phanerodontia chrysosporium
3.1.1.117 molecular biology the enzyme may prove a valuable as research tool for the investigation of lignin and lignin to carbohydrates-bond chemistry Trichoderma reesei
3.1.1.117 molecular biology the enzyme may prove a valuable as research tool for the investigation of lignin and lignin to carbohydrates-bond chemistry Schizophyllum commune
3.1.1.117 molecular biology the enzyme may prove a valuable research tool for the investigation of lignin and lignin to carbohydrates-bond chemistry Phanerodontia chrysosporium
3.1.1.117 molecular biology the enzyme may prove valuable as a research tool for the investigation of lignin and lignin to carbohydrates-bond chemistry Thermothelomyces thermophilus
3.1.1.117 molecular biology the enzyme may prove valuable as a research tool for the investigation of lignin and lignin to carbohydrates-bond chemistry Podospora anserina
3.1.1.117 molecular biology the enzyme may prove valuable as a research tool for the investigation of lignin and lignin to carbohydrates-bond chemistry Cerrena unicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.117 additional information
-
additional information Michaelis-Menten kinetics Thermothelomyces thermophilus
3.1.1.117 additional information
-
additional information Michaelis-Menten kinetics Podospora anserina
3.1.1.117 8.9
-
1-(4-hydroxy-3-methoxyphenyl)-1-(alpha-D-glucuronate)-2-(2-methoxyphenoxy)-3-propanol pH 6.0, 45°C Thermothelomyces thermophilus
3.1.1.117 12.1
-
1-(4-hydroxy-3-methoxyphenyl)-1-(alpha-D-glucuronate)-2-(2-methoxyphenoxy)-3-propanol pH 6.0, 35°C Podospora anserina

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.117 Cerrena unicolor A0A0A7EQR3
-
-
3.1.1.117 Phanerodontia chrysosporium P0CT87
-
-
3.1.1.117 Phanerodontia chrysosporium P0CT88
-
-
3.1.1.117 Phanerodontia chrysosporium RP-78 P0CT87
-
-
3.1.1.117 Podospora anserina B2ABS0
-
-
3.1.1.117 Podospora anserina B2ABS0 Pleurage anserina
-
3.1.1.117 Podospora anserina ATCC MYA-4624 B2ABS0 Pleurage anserina
-
3.1.1.117 Podospora anserina DSM 980 B2ABS0
-
-
3.1.1.117 Podospora anserina DSM 980 B2ABS0 Pleurage anserina
-
3.1.1.117 Podospora anserina FGSC 10383 B2ABS0 Pleurage anserina
-
3.1.1.117 Podospora anserina S B2ABS0 Pleurage anserina
-
3.1.1.117 Schizophyllum commune D8QLP9
-
-
3.1.1.117 Schizophyllum commune H4-8 D8QLP9
-
-
3.1.1.117 Thermothelomyces thermophilus G2QJR6
-
-
3.1.1.117 Thermothelomyces thermophilus G2QJR6 Sporotrichum thermophile
-
3.1.1.117 Thermothelomyces thermophilus ATCC 42464 G2QJR6 Sporotrichum thermophile
-
3.1.1.117 Thermothelomyces thermophilus BCRC 31852 G2QJR6 Sporotrichum thermophile
-
3.1.1.117 Thermothelomyces thermophilus DSM 1799 G2QJR6
-
-
3.1.1.117 Thermothelomyces thermophilus DSM 1799 G2QJR6 Sporotrichum thermophile
-
3.1.1.117 Trichoderma reesei G0RV93
-
-
3.1.1.117 Trichoderma reesei QM6a G0RV93
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.117 1-(4-hydroxy-3-methoxyphenyl)-1-(alpha-D-glucuronate)-2-(2-methoxyphenoxy)-3-propanol + H2O
-
Thermothelomyces thermophilus ?
-
?
3.1.1.117 1-(4-hydroxy-3-methoxyphenyl)-1-(alpha-D-glucuronate)-2-(2-methoxyphenoxy)-3-propanol + H2O
-
Podospora anserina ?
-
?
3.1.1.117 1-(4-hydroxy-3-methoxyphenyl)-1-(alpha-D-glucuronate)-2-(2-methoxyphenoxy)-3-propanol + H2O
-
Thermothelomyces thermophilus ATCC 42464 ?
-
?
3.1.1.117 1-(4-hydroxy-3-methoxyphenyl)-1-(alpha-D-glucuronate)-2-(2-methoxyphenoxy)-3-propanol + H2O
-
Podospora anserina ATCC MYA-4624 ?
-
?
3.1.1.117 1-(4-hydroxy-3-methoxyphenyl)-1-(alpha-D-glucuronate)-2-(2-methoxyphenoxy)-3-propanol + H2O
-
Podospora anserina DSM 980 ?
-
?
3.1.1.117 1-(4-hydroxy-3-methoxyphenyl)-1-(alpha-D-glucuronate)-2-(2-methoxyphenoxy)-3-propanol + H2O
-
Podospora anserina FGSC 10383 ?
-
?
3.1.1.117 1-(4-hydroxy-3-methoxyphenyl)-1-(alpha-D-glucuronate)-2-(2-methoxyphenoxy)-3-propanol + H2O
-
Thermothelomyces thermophilus BCRC 31852 ?
-
?
3.1.1.117 1-(4-hydroxy-3-methoxyphenyl)-1-(alpha-D-glucuronate)-2-(2-methoxyphenoxy)-3-propanol + H2O
-
Podospora anserina S ?
-
?
3.1.1.117 1-(4-hydroxy-3-methoxyphenyl)-1-(alpha-D-glucuronate)-2-(2-methoxyphenoxy)-3-propanol + H2O
-
Thermothelomyces thermophilus DSM 1799 ?
-
?
3.1.1.117 benzyl D-glucuronic acid ester + H2O the application of this substrate is described (a) in a Thin Layer Chromatography (TLC) assay for qualitative activity assessment (b) a spectrophotometer-based assay useful for screening, and (c) an HPLC-based assay allowing more precise activity determinations, enabling enzyme kinetics characterization. In addition, TLC and spectrophotometric methods are less costly and time consuming, and there is no need for complicated instruments for the analysis Trichoderma reesei ?
-
?
3.1.1.117 benzyl D-glucuronic acid ester + H2O the application of this substrate is described (a) in a Thin Layer Chromatography (TLC) assay for qualitative activity assessment (b) a spectrophotometer-based assay useful for screening, and (c) an HPLC-based assay allowing more precise activity determinations, enabling enzyme kinetics characterization. In addition, TLC and spectrophotometric methods are less costly and time consuming, and there is no need for complicated instruments for the analysis Phanerodontia chrysosporium ?
-
?
3.1.1.117 benzyl D-glucuronic acid ester + H2O the application of this substrate is described (a) in a Thin Layer Chromatography (TLC) assay for qualitative activity assessment (b) a spectrophotometer-based assay useful for screening, and (c) an HPLC-based assay allowing more precise activity determinations, enabling enzyme kinetics characterization. In addition, TLC and spectrophotometric methods are less costly and time consuming, and there is no need for complicated instruments for the analysis Trichoderma reesei QM6a ?
-
?
3.1.1.117 benzyl D-glucuronic acid ester + H2O the application of this substrate is described (a) in a Thin Layer Chromatography (TLC) assay for qualitative activity assessment (b) a spectrophotometer-based assay useful for screening, and (c) an HPLC-based assay allowing more precise activity determinations, enabling enzyme kinetics characterization. In addition, TLC and spectrophotometric methods are less costly and time consuming, and there is no need for complicated instruments for the analysis Thermothelomyces thermophilus benzyl alcohol + D-glucuronic acid
-
?
3.1.1.117 benzyl D-glucuronic acid ester + H2O the application of this substrate is described (a) in a Thin Layer Chromatography (TLC) assay for qualitative activity assessment (b) a spectrophotometer-based assay useful for screening, and (c) an HPLC-based assay allowing more precise activity determinations, enabling enzyme kinetics characterization. In addition, TLC and spectrophotometric methods are less costly and time consuming, and there is no need for complicated instruments for the analysis Podospora anserina benzyl alcohol + D-glucuronic acid
-
?
3.1.1.117 benzyl D-glucuronic acid ester + H2O the application of this substrate is described (a) in a Thin Layer Chromatography (TLC) assay for qualitative activity assessment (b) a spectrophotometer-based assay useful for screening, and (c) an HPLC-based assay allowing more precise activity determinations, enabling enzyme kinetics characterization. In addition, TLC and spectrophotometric methods are less costly and time consuming, and there is no need for complicated instruments for the analysis Cerrena unicolor benzyl alcohol + D-glucuronic acid
-
?
3.1.1.117 benzyl D-glucuronic acid ester + H2O the application of this substrate is described (a) in a Thin Layer Chromatography (TLC) assay for qualitative activity assessment (b) a spectrophotometer-based assay useful for screening, and (c) an HPLC-based assay allowing more precise activity determinations, enabling enzyme kinetics characterization. In addition, TLC and spectrophotometric methods are less costly and time consuming, and there is no need for complicated instruments for the analysis Phanerodontia chrysosporium benzyl alcohol + D-glucuronic acid
-
?
3.1.1.117 benzyl D-glucuronic acid ester + H2O the application of this substrate is described (a) in a Thin Layer Chromatography (TLC) assay for qualitative activity assessment (b) a spectrophotometer-based assay useful for screening, and (c) an HPLC-based assay allowing more precise activity determinations, enabling enzyme kinetics characterization. In addition, TLC and spectrophotometric methods are less costly and time consuming, and there is no need for complicated instruments for the analysis Thermothelomyces thermophilus ATCC 42464 benzyl alcohol + D-glucuronic acid
-
?
3.1.1.117 benzyl D-glucuronic acid ester + H2O the application of this substrate is described (a) in a Thin Layer Chromatography (TLC) assay for qualitative activity assessment (b) a spectrophotometer-based assay useful for screening, and (c) an HPLC-based assay allowing more precise activity determinations, enabling enzyme kinetics characterization. In addition, TLC and spectrophotometric methods are less costly and time consuming, and there is no need for complicated instruments for the analysis Podospora anserina ATCC MYA-4624 benzyl alcohol + D-glucuronic acid
-
?
3.1.1.117 benzyl D-glucuronic acid ester + H2O the application of this substrate is described (a) in a Thin Layer Chromatography (TLC) assay for qualitative activity assessment (b) a spectrophotometer-based assay useful for screening, and (c) an HPLC-based assay allowing more precise activity determinations, enabling enzyme kinetics characterization. In addition, TLC and spectrophotometric methods are less costly and time consuming, and there is no need for complicated instruments for the analysis Podospora anserina DSM 980 benzyl alcohol + D-glucuronic acid
-
?
3.1.1.117 benzyl D-glucuronic acid ester + H2O the application of this substrate is described (a) in a Thin Layer Chromatography (TLC) assay for qualitative activity assessment (b) a spectrophotometer-based assay useful for screening, and (c) an HPLC-based assay allowing more precise activity determinations, enabling enzyme kinetics characterization. In addition, TLC and spectrophotometric methods are less costly and time consuming, and there is no need for complicated instruments for the analysis Podospora anserina FGSC 10383 benzyl alcohol + D-glucuronic acid
-
?
3.1.1.117 benzyl D-glucuronic acid ester + H2O the application of this substrate is described (a) in a Thin Layer Chromatography (TLC) assay for qualitative activity assessment (b) a spectrophotometer-based assay useful for screening, and (c) an HPLC-based assay allowing more precise activity determinations, enabling enzyme kinetics characterization. In addition, TLC and spectrophotometric methods are less costly and time consuming, and there is no need for complicated instruments for the analysis Thermothelomyces thermophilus BCRC 31852 benzyl alcohol + D-glucuronic acid
-
?
3.1.1.117 benzyl D-glucuronic acid ester + H2O the application of this substrate is described (a) in a Thin Layer Chromatography (TLC) assay for qualitative activity assessment (b) a spectrophotometer-based assay useful for screening, and (c) an HPLC-based assay allowing more precise activity determinations, enabling enzyme kinetics characterization. In addition, TLC and spectrophotometric methods are less costly and time consuming, and there is no need for complicated instruments for the analysis Podospora anserina S benzyl alcohol + D-glucuronic acid
-
?
3.1.1.117 benzyl D-glucuronic acid ester + H2O the application of this substrate is described (a) in a Thin Layer Chromatography (TLC) assay for qualitative activity assessment (b) a spectrophotometer-based assay useful for screening, and (c) an HPLC-based assay allowing more precise activity determinations, enabling enzyme kinetics characterization. In addition, TLC and spectrophotometric methods are less costly and time consuming, and there is no need for complicated instruments for the analysis Thermothelomyces thermophilus DSM 1799 benzyl alcohol + D-glucuronic acid
-
?
3.1.1.117 benzyl D-glucuronic acid ester + H2O the application of this substrate is described (a) in a Thin Layer Chromatography (TLC) assay for qualitative activity assessment (b) a spectrophotometer-based assay useful for screening, and (c) an HPLC-based assay allowing more precise activity determinations, enabling enzyme kinetics characterization. In addition, TLC and spectrophotometric methods are less costly and time consuming, and there is no need for complicated instruments for the analysis Phanerodontia chrysosporium RP-78 benzyl alcohol + D-glucuronic acid
-
?
3.1.1.117 benzyl D-glucuronic acid ester + H2O the application of this substrate is described (a) in a Thin Layer Chromatography (TLC) assay for qualitative activity assessment (b) a spectrophotometer-based assay useful for screening, and (c) an HPLC-based assay allowing more precise activity determinations, enabling enzyme kinetics characterization. In addition, TLC and spectrophotometric methods are less costly and time consuming, and there is no need for complicated instruments for the analysis Schizophyllum commune benzyl alcohol + glucuronic acid
-
?
3.1.1.117 benzyl D-glucuronic acid ester + H2O the application of this substrate is described (a) in a Thin Layer Chromatography (TLC) assay for qualitative activity assessment (b) a spectrophotometer-based assay useful for screening, and (c) an HPLC-based assay allowing more precise activity determinations, enabling enzyme kinetics characterization. In addition, TLC and spectrophotometric methods are less costly and time consuming, and there is no need for complicated instruments for the analysis Schizophyllum commune H4-8 benzyl alcohol + glucuronic acid
-
?
3.1.1.117 benzyl-D-glucuronate + H2O
-
Thermothelomyces thermophilus benzyl alcohol + D-glucuronic acid
-
?
3.1.1.117 benzyl-D-glucuronate + H2O
-
Podospora anserina benzyl alcohol + D-glucuronic acid
-
?
3.1.1.117 benzyl-D-glucuronate + H2O
-
Thermothelomyces thermophilus ATCC 42464 benzyl alcohol + D-glucuronic acid
-
?
3.1.1.117 benzyl-D-glucuronate + H2O
-
Podospora anserina ATCC MYA-4624 benzyl alcohol + D-glucuronic acid
-
?
3.1.1.117 benzyl-D-glucuronate + H2O
-
Podospora anserina DSM 980 benzyl alcohol + D-glucuronic acid
-
?
3.1.1.117 benzyl-D-glucuronate + H2O
-
Podospora anserina FGSC 10383 benzyl alcohol + D-glucuronic acid
-
?
3.1.1.117 benzyl-D-glucuronate + H2O
-
Thermothelomyces thermophilus BCRC 31852 benzyl alcohol + D-glucuronic acid
-
?
3.1.1.117 benzyl-D-glucuronate + H2O
-
Podospora anserina S benzyl alcohol + D-glucuronic acid
-
?
3.1.1.117 benzyl-D-glucuronate + H2O
-
Thermothelomyces thermophilus DSM 1799 benzyl alcohol + D-glucuronic acid
-
?
3.1.1.117 additional information design of BnGlcA-based glucuronoyl esterase (GE) assays for different applications: a thin-layer chromatography assay for qualitative activity detection, a coupled-enzyme spectrophotometric assay that can be used for high-throughput screening or general activity determinations and a HPLC-based detection method allowing kinetic determinations. The three-level experimental procedure not merely facilitates routine, fast and simple biochemical characterizations but it can also give rise to the discovery different GEs through an extensive screening of heterologous genomic and metagenomic expression libraries Thermothelomyces thermophilus ?
-
-
3.1.1.117 additional information design of BnGlcA-based glucuronoyl esterase (GE) assays for different applications: a thin-layer chromatography assay for qualitative activity detection, a coupled-enzyme spectrophotometric assay that can be used for high-throughput screening or general activity determinations and a HPLC-based detection method allowing kinetic determinations. The three-level experimental procedure not merely facilitates routine, fast and simple biochemical characterizations but it can also give rise to the discovery different GEs through an extensive screening of heterologous genomic and metagenomic expression libraries Podospora anserina ?
-
-
3.1.1.117 additional information design of BnGlcA-based glucuronoyl esterase (GE) assays for different applications: a thin-layer chromatography assay for qualitative activity detection, a coupled-enzyme spectrophotometric assay that can be used for high-throughput screening or general activity determinations and a HPLC-based detection method allowing kinetic determinations. The three-level experimental procedure not merely facilitates routine, fast and simple biochemical characterizations but it can also give rise to the discovery different GEs through an extensive screening of heterologous genomic and metagenomic expression libraries Thermothelomyces thermophilus ATCC 42464 ?
-
-
3.1.1.117 additional information design of BnGlcA-based glucuronoyl esterase (GE) assays for different applications: a thin-layer chromatography assay for qualitative activity detection, a coupled-enzyme spectrophotometric assay that can be used for high-throughput screening or general activity determinations and a HPLC-based detection method allowing kinetic determinations. The three-level experimental procedure not merely facilitates routine, fast and simple biochemical characterizations but it can also give rise to the discovery different GEs through an extensive screening of heterologous genomic and metagenomic expression libraries Podospora anserina ATCC MYA-4624 ?
-
-
3.1.1.117 additional information design of BnGlcA-based glucuronoyl esterase (GE) assays for different applications: a thin-layer chromatography assay for qualitative activity detection, a coupled-enzyme spectrophotometric assay that can be used for high-throughput screening or general activity determinations and a HPLC-based detection method allowing kinetic determinations. The three-level experimental procedure not merely facilitates routine, fast and simple biochemical characterizations but it can also give rise to the discovery different GEs through an extensive screening of heterologous genomic and metagenomic expression libraries Podospora anserina DSM 980 ?
-
-
3.1.1.117 additional information design of BnGlcA-based glucuronoyl esterase (GE) assays for different applications: a thin-layer chromatography assay for qualitative activity detection, a coupled-enzyme spectrophotometric assay that can be used for high-throughput screening or general activity determinations and a HPLC-based detection method allowing kinetic determinations. The three-level experimental procedure not merely facilitates routine, fast and simple biochemical characterizations but it can also give rise to the discovery different GEs through an extensive screening of heterologous genomic and metagenomic expression libraries Podospora anserina FGSC 10383 ?
-
-
3.1.1.117 additional information design of BnGlcA-based glucuronoyl esterase (GE) assays for different applications: a thin-layer chromatography assay for qualitative activity detection, a coupled-enzyme spectrophotometric assay that can be used for high-throughput screening or general activity determinations and a HPLC-based detection method allowing kinetic determinations. The three-level experimental procedure not merely facilitates routine, fast and simple biochemical characterizations but it can also give rise to the discovery different GEs through an extensive screening of heterologous genomic and metagenomic expression libraries Thermothelomyces thermophilus BCRC 31852 ?
-
-
3.1.1.117 additional information design of BnGlcA-based glucuronoyl esterase (GE) assays for different applications: a thin-layer chromatography assay for qualitative activity detection, a coupled-enzyme spectrophotometric assay that can be used for high-throughput screening or general activity determinations and a HPLC-based detection method allowing kinetic determinations. The three-level experimental procedure not merely facilitates routine, fast and simple biochemical characterizations but it can also give rise to the discovery different GEs through an extensive screening of heterologous genomic and metagenomic expression libraries Podospora anserina S ?
-
-
3.1.1.117 additional information design of BnGlcA-based glucuronoyl esterase (GE) assays for different applications: a thin-layer chromatography assay for qualitative activity detection, a coupled-enzyme spectrophotometric assay that can be used for high-throughput screening or general activity determinations and a HPLC-based detection method allowing kinetic determinations. The three-level experimental procedure not merely facilitates routine, fast and simple biochemical characterizations but it can also give rise to the discovery different GEs through an extensive screening of heterologous genomic and metagenomic expression libraries Thermothelomyces thermophilus DSM 1799 ?
-
-

Synonyms

EC Number Synonyms Comment Organism
3.1.1.117 4-O-methyl-glucuronoyl methylesterase UniProt Thermothelomyces thermophilus
3.1.1.117 4-O-methyl-glucuronoyl methylesterase UniProt Podospora anserina
3.1.1.117 CuGE
-
Cerrena unicolor
3.1.1.117 GE Cip2
-
Trichoderma reesei
3.1.1.117 GE1
-
Podospora anserina
3.1.1.117 Ge2
-
Thermothelomyces thermophilus
3.1.1.117 glucuronoyl esterase
-
Thermothelomyces thermophilus
3.1.1.117 glucuronoyl esterase
-
Podospora anserina
3.1.1.117 glucuronoyl esterase 1
-
Podospora anserina
3.1.1.117 glucuronoyl esterase 2
-
Thermothelomyces thermophilus
3.1.1.117 PaGE1
-
Podospora anserina
3.1.1.117 PcGE1
-
Phanerodontia chrysosporium
3.1.1.117 PcGE2
-
Phanerodontia chrysosporium
3.1.1.117 ScGE1
-
Schizophyllum commune
3.1.1.117 StGE2
-
Thermothelomyces thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.117 35
-
assay at Podospora anserina
3.1.1.117 45
-
assay at Thermothelomyces thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.117 3.5
-
1-(4-hydroxy-3-methoxyphenyl)-1-(alpha-D-glucuronate)-2-(2-methoxyphenoxy)-3-propanol pH 6.0, 45°C Thermothelomyces thermophilus
3.1.1.117 7.8
-
1-(4-hydroxy-3-methoxyphenyl)-1-(alpha-D-glucuronate)-2-(2-methoxyphenoxy)-3-propanol pH 6.0, 35°C Podospora anserina

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.117 6
-
assay at Thermothelomyces thermophilus
3.1.1.117 6
-
assay at Podospora anserina