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Literature summary extracted from

  • Sanders, B.D.; Zhao, K.; Slama, J.T.; Marmorstein, R.
    Structural basis for nicotinamide inhibition and base exchange in Sir2 enzymes (2007), Mol. Cell, 25, 463-472 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.286 enzyme in complex with its acetyl-lysine 16 histone H4 substrate, nicotinamide and the reaction intermediate analog ADP-HPD, vapor diffusion method, using 2.0 M (NH4)2SO4, 100 mM Na citrate, pH 5.6, 200 mM K/Na tartrate or 2.0 M (NH4)2SO4 and 100 mM Na citrate, pH 5.5 Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.286 D118N the mutant retains approximately 13% of the activity of the wild type protein Saccharomyces cerevisiae
2.3.1.286 I117A inactive Saccharomyces cerevisiae
2.3.1.286 I117D inactive Saccharomyces cerevisiae
2.3.1.286 I117F the mutant is nearly as active as the wild type protein (about 75% activity) Saccharomyces cerevisiae
2.3.1.286 I117H inactive Saccharomyces cerevisiae
2.3.1.286 I117L the mutant retains approximately 25% of the activity of the wild type protein Saccharomyces cerevisiae
2.3.1.286 I117V the mutant is nearly as active as the wild type protein (about 65% activity) Saccharomyces cerevisiae
2.3.1.286 I117W inactive Saccharomyces cerevisiae
2.3.1.286 I117Y inactive Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.286 nicotinamide noncompetitive inhibitor Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.286 0.0161
-
 NAD+ wild type enzyme, at pH 8.0, temperature not specified in the publication Saccharomyces cerevisiae
2.3.1.286 0.0255
-
 NAD+ mutant enzyme I117V, at pH 8.0, temperature not specified in the publication Saccharomyces cerevisiae
2.3.1.286 0.0258
-
 NAD+ mutant enzyme I117F, at pH 8.0, temperature not specified in the publication Saccharomyces cerevisiae
2.3.1.286 0.4535
-
 NAD+ mutant enzyme D118N, at pH 8.0, temperature not specified in the publication Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.286 Saccharomyces cerevisiae P53686
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.286 fluorogenic acetyl-L-lysine + NAD+ + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
2.3.1.286 [histone H4]-N6-acetyl-L-lysine16 + NAD+ + H2O
-
 Saccharomyces cerevisiae [histone H4]-L-lysine16 + 2''-O-acetyl-ADP-D-ribose + nicotinamide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.286 HST2
-
 Saccharomyces cerevisiae
2.3.1.286 Sir2
-
 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.286 NAD+
-
 Saccharomyces cerevisiae