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Literature summary extracted from

  • Li, W.; Ji, P.; Zhou, Q.; Hua, C.; Han, C.
    Insights into the synergistic biodegradation of waste papers using a combination of thermostable endoglucanase and cellobiohydrolase from Chaetomium thermophilum (2018), Mol. Biotechnol., 60, 49-54 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 recombinant expression of C-termially His6-tagged enzyme in Pichia pastoris strain GS115 Thermochaetoides thermophila

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 Ag+ slight inhibition at 10 mM Thermochaetoides thermophila
3.2.1.4 Al3+ slight inhibition at 10 mM Thermochaetoides thermophila
3.2.1.4 Co2+ activates 38% at 2 mM, inhibits 20% at 10 mM Thermochaetoides thermophila
3.2.1.4 Cr3+
-
Thermochaetoides thermophila
3.2.1.4 Cu2+ complete inhibition at 2-10 mM Thermochaetoides thermophila
3.2.1.4 Fe3+ complete inhibition at 2-10 mM Thermochaetoides thermophila
3.2.1.4 Ni2+ activates 8% at 2 mM, inhibits 8% at 10 mM Thermochaetoides thermophila
3.2.1.4 Pb2+
-
Thermochaetoides thermophila

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.4 Ca2+ activates Thermochaetoides thermophila
3.2.1.4 Co2+ activates 38% at 2 mM, inhibits 20% at 10 mM Thermochaetoides thermophila
3.2.1.4 Mn2+ activates Thermochaetoides thermophila
3.2.1.4 Sr2+ activates Thermochaetoides thermophila
3.2.1.4 Zn2+ activates Thermochaetoides thermophila

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Thermochaetoides thermophila G0S9G3
-
-
3.2.1.4 Thermochaetoides thermophila CBS 144.50 G0S9G3
-
-
3.2.1.4 Thermochaetoides thermophila DSM 1495 G0S9G3
-
-
3.2.1.4 Thermochaetoides thermophila IMI 039719 G0S9G3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4 recombinant His-tagged enzyme from Pichia pastoris strain GS115 by nickel affinity chromatography Thermochaetoides thermophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 carboxymethyl cellulose + H2O
-
Thermochaetoides thermophila ?
-
?
3.2.1.4 carboxymethyl cellulose + H2O
-
Thermochaetoides thermophila IMI 039719 ?
-
?
3.2.1.4 carboxymethyl cellulose + H2O
-
Thermochaetoides thermophila DSM 1495 ?
-
?
3.2.1.4 carboxymethyl cellulose + H2O
-
Thermochaetoides thermophila CBS 144.50 ?
-
?
3.2.1.4 additional information synergistic biodegradation of waste papers using a combination of thermostable endoglucanase CTendo45 and cellobiohydrolase CtCel6 from Chaetomium thermophilum. CtCel6 significantly enhances the bioconversion process, and CTendo45 synergistically increases the degradation, with a maximum degree of synergistic effect of 1.67 when the mass ratio of CTendo45/CtCel6 is 5:3. Enzymatic hydrolysis of different paper materials, e.g. filter paper and office paper giving high activity, or newspaper and cardboard resulting in low activity, foolscap paper gives moderate activity. For newspaper, the recalcitrance to the enzymatic action may be caused by the high contents of hemicelluloses, lignin and various inorganic components Thermochaetoides thermophila ?
-
?
3.2.1.4 additional information synergistic biodegradation of waste papers using a combination of thermostable endoglucanase CTendo45 and cellobiohydrolase CtCel6 from Chaetomium thermophilum. CtCel6 significantly enhances the bioconversion process, and CTendo45 synergistically increases the degradation, with a maximum degree of synergistic effect of 1.67 when the mass ratio of CTendo45/CtCel6 is 5:3. Enzymatic hydrolysis of different paper materials, e.g. filter paper and office paper giving high activity, or newspaper and cardboard resulting in low activity, foolscap paper gives moderate activity. For newspaper, the recalcitrance to the enzymatic action may be caused by the high contents of hemicelluloses, lignin and various inorganic components Thermochaetoides thermophila IMI 039719 ?
-
?
3.2.1.4 additional information synergistic biodegradation of waste papers using a combination of thermostable endoglucanase CTendo45 and cellobiohydrolase CtCel6 from Chaetomium thermophilum. CtCel6 significantly enhances the bioconversion process, and CTendo45 synergistically increases the degradation, with a maximum degree of synergistic effect of 1.67 when the mass ratio of CTendo45/CtCel6 is 5:3. Enzymatic hydrolysis of different paper materials, e.g. filter paper and office paper giving high activity, or newspaper and cardboard resulting in low activity, foolscap paper gives moderate activity. For newspaper, the recalcitrance to the enzymatic action may be caused by the high contents of hemicelluloses, lignin and various inorganic components Thermochaetoides thermophila DSM 1495 ?
-
?
3.2.1.4 additional information synergistic biodegradation of waste papers using a combination of thermostable endoglucanase CTendo45 and cellobiohydrolase CtCel6 from Chaetomium thermophilum. CtCel6 significantly enhances the bioconversion process, and CTendo45 synergistically increases the degradation, with a maximum degree of synergistic effect of 1.67 when the mass ratio of CTendo45/CtCel6 is 5:3. Enzymatic hydrolysis of different paper materials, e.g. filter paper and office paper giving high activity, or newspaper and cardboard resulting in low activity, foolscap paper gives moderate activity. For newspaper, the recalcitrance to the enzymatic action may be caused by the high contents of hemicelluloses, lignin and various inorganic components Thermochaetoides thermophila CBS 144.50 ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 CTendo45
-
Thermochaetoides thermophila
3.2.1.4 ctendo7
-
Thermochaetoides thermophila
3.2.1.4 CTHT_0045780 gene name, UniProt Thermochaetoides thermophila
3.2.1.4 endoglucanase
-
Thermochaetoides thermophila

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 50
-
in vivo assay at Thermochaetoides thermophila