EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.4 | recombinant expression of C-termially His6-tagged enzyme in Pichia pastoris strain GS115 | Thermochaetoides thermophila |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.4 | Ag+ | slight inhibition at 10 mM | Thermochaetoides thermophila | |
3.2.1.4 | Al3+ | slight inhibition at 10 mM | Thermochaetoides thermophila | |
3.2.1.4 | Co2+ | activates 38% at 2 mM, inhibits 20% at 10 mM | Thermochaetoides thermophila | |
3.2.1.4 | Cr3+ | - |
Thermochaetoides thermophila | |
3.2.1.4 | Cu2+ | complete inhibition at 2-10 mM | Thermochaetoides thermophila | |
3.2.1.4 | Fe3+ | complete inhibition at 2-10 mM | Thermochaetoides thermophila | |
3.2.1.4 | Ni2+ | activates 8% at 2 mM, inhibits 8% at 10 mM | Thermochaetoides thermophila | |
3.2.1.4 | Pb2+ | - |
Thermochaetoides thermophila |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.4 | Ca2+ | activates | Thermochaetoides thermophila | |
3.2.1.4 | Co2+ | activates 38% at 2 mM, inhibits 20% at 10 mM | Thermochaetoides thermophila | |
3.2.1.4 | Mn2+ | activates | Thermochaetoides thermophila | |
3.2.1.4 | Sr2+ | activates | Thermochaetoides thermophila | |
3.2.1.4 | Zn2+ | activates | Thermochaetoides thermophila |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.4 | Thermochaetoides thermophila | G0S9G3 | - |
- |
3.2.1.4 | Thermochaetoides thermophila CBS 144.50 | G0S9G3 | - |
- |
3.2.1.4 | Thermochaetoides thermophila DSM 1495 | G0S9G3 | - |
- |
3.2.1.4 | Thermochaetoides thermophila IMI 039719 | G0S9G3 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.4 | recombinant His-tagged enzyme from Pichia pastoris strain GS115 by nickel affinity chromatography | Thermochaetoides thermophila |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.4 | carboxymethyl cellulose + H2O | - |
Thermochaetoides thermophila | ? | - |
? | |
3.2.1.4 | carboxymethyl cellulose + H2O | - |
Thermochaetoides thermophila IMI 039719 | ? | - |
? | |
3.2.1.4 | carboxymethyl cellulose + H2O | - |
Thermochaetoides thermophila DSM 1495 | ? | - |
? | |
3.2.1.4 | carboxymethyl cellulose + H2O | - |
Thermochaetoides thermophila CBS 144.50 | ? | - |
? | |
3.2.1.4 | additional information | synergistic biodegradation of waste papers using a combination of thermostable endoglucanase CTendo45 and cellobiohydrolase CtCel6 from Chaetomium thermophilum. CtCel6 significantly enhances the bioconversion process, and CTendo45 synergistically increases the degradation, with a maximum degree of synergistic effect of 1.67 when the mass ratio of CTendo45/CtCel6 is 5:3. Enzymatic hydrolysis of different paper materials, e.g. filter paper and office paper giving high activity, or newspaper and cardboard resulting in low activity, foolscap paper gives moderate activity. For newspaper, the recalcitrance to the enzymatic action may be caused by the high contents of hemicelluloses, lignin and various inorganic components | Thermochaetoides thermophila | ? | - |
? | |
3.2.1.4 | additional information | synergistic biodegradation of waste papers using a combination of thermostable endoglucanase CTendo45 and cellobiohydrolase CtCel6 from Chaetomium thermophilum. CtCel6 significantly enhances the bioconversion process, and CTendo45 synergistically increases the degradation, with a maximum degree of synergistic effect of 1.67 when the mass ratio of CTendo45/CtCel6 is 5:3. Enzymatic hydrolysis of different paper materials, e.g. filter paper and office paper giving high activity, or newspaper and cardboard resulting in low activity, foolscap paper gives moderate activity. For newspaper, the recalcitrance to the enzymatic action may be caused by the high contents of hemicelluloses, lignin and various inorganic components | Thermochaetoides thermophila IMI 039719 | ? | - |
? | |
3.2.1.4 | additional information | synergistic biodegradation of waste papers using a combination of thermostable endoglucanase CTendo45 and cellobiohydrolase CtCel6 from Chaetomium thermophilum. CtCel6 significantly enhances the bioconversion process, and CTendo45 synergistically increases the degradation, with a maximum degree of synergistic effect of 1.67 when the mass ratio of CTendo45/CtCel6 is 5:3. Enzymatic hydrolysis of different paper materials, e.g. filter paper and office paper giving high activity, or newspaper and cardboard resulting in low activity, foolscap paper gives moderate activity. For newspaper, the recalcitrance to the enzymatic action may be caused by the high contents of hemicelluloses, lignin and various inorganic components | Thermochaetoides thermophila DSM 1495 | ? | - |
? | |
3.2.1.4 | additional information | synergistic biodegradation of waste papers using a combination of thermostable endoglucanase CTendo45 and cellobiohydrolase CtCel6 from Chaetomium thermophilum. CtCel6 significantly enhances the bioconversion process, and CTendo45 synergistically increases the degradation, with a maximum degree of synergistic effect of 1.67 when the mass ratio of CTendo45/CtCel6 is 5:3. Enzymatic hydrolysis of different paper materials, e.g. filter paper and office paper giving high activity, or newspaper and cardboard resulting in low activity, foolscap paper gives moderate activity. For newspaper, the recalcitrance to the enzymatic action may be caused by the high contents of hemicelluloses, lignin and various inorganic components | Thermochaetoides thermophila CBS 144.50 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.4 | CTendo45 | - |
Thermochaetoides thermophila |
3.2.1.4 | ctendo7 | - |
Thermochaetoides thermophila |
3.2.1.4 | CTHT_0045780 | gene name, UniProt | Thermochaetoides thermophila |
3.2.1.4 | endoglucanase | - |
Thermochaetoides thermophila |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.4 | 50 | - |
in vivo assay at | Thermochaetoides thermophila |