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Literature summary extracted from

  • Wang, L.; Li, S.; Zhang, S.; Li, J.; Yu, W.; Gong, Q.
    A new kappa-carrageenase CgkS from marine bacterium Shewanella sp. Kz7 (2015), J. Ocean Univ. China, 14, 759-763 .
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.83 NaCl the enzyme does not require NaCl for activity, although its activity is enhanced by NaCl (226.8% activity at 200 mM) Shewanella sp. Kz7

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.83 expressed in Escherichia coli BL21(DE3) cells Shewanella sp. Kz7

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.83 Al3+ 72.3% residual activity at 1 mM Shewanella sp. Kz7
3.2.1.83 Cu2+ 26.3% residual activity at 1 mM Shewanella sp. Kz7
3.2.1.83 EDTA 90.8% residual activity at 1 mM Shewanella sp. Kz7
3.2.1.83 Fe2+ 83.8% residual activity at 1 mM Shewanella sp. Kz7
3.2.1.83 K+ 82.6% residual activity at 1 mM Shewanella sp. Kz7
3.2.1.83 Li+ 92.1% residual activity at 1 mM Shewanella sp. Kz7
3.2.1.83 Mg2+ 83.3% residual activity at 1 mM Shewanella sp. Kz7
3.2.1.83 NH4+ 81.1% residual activity at 1 mM Shewanella sp. Kz7
3.2.1.83 SDS 21.1% residual activity at 1 mM Shewanella sp. Kz7
3.2.1.83 Zn2+ 35.4% residual activity at 1 mM Shewanella sp. Kz7

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.83 Ca2+ 132.4% activity at 1 mM Shewanella sp. Kz7
3.2.1.83 Mn2+ 109.2% activity at 1 mM Shewanella sp. Kz7

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.83 kappa-carrageen + H2O Shewanella sp. Kz7
-
 kappa-neocarratetraose + kappa-neocarrabiose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.83 Shewanella sp. Kz7 W8V7X4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.83 Ni-Sepharose column chromatography Shewanella sp. Kz7

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.83 kappa-carrageen + H2O
-
 Shewanella sp. Kz7 kappa-neocarratetraose + kappa-neocarrabiose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.83 ? x * 45000, SDS-PAGE Shewanella sp. Kz7
3.2.1.83 ? x * 42743, calculated from amino acid sequence Shewanella sp. Kz7

Synonyms

EC Number Synonyms Comment Organism
3.2.1.83 CgkS
-
 Shewanella sp. Kz7

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.83 45
-
-
 Shewanella sp. Kz7

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.83 30
-
 85% of the enzymatic activities remain after being incubated at 30°C for 1 h Shewanella sp. Kz7

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.83 8
-
 in phosphate buffer Shewanella sp. Kz7

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.83 6 9 the enzyme is stable within a range of pH 6.0-9.0 Shewanella sp. Kz7

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.83 Shewanella sp. Kz7 calculated from amino acid sequence
-
 9.1