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Literature summary extracted from

  • Chai, S.; Abu Bakar, F.; Mahadi, N.; Murad, A.
    A thermotolerant endo-1,4-beta-mannanase from Trichoderma virens UKM1 Cloning, recombinant expression and characterization (2016), J. Mol. Catal. B, 125, 49-57 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.78 expressed in Pichia pastoris X33 cells Trichoderma virens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.78 Trichoderma virens A0A0N9LQI1
-
-
3.2.1.78 Trichoderma virens UKM1 A0A0N9LQI1
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.78 glycoprotein
-
 Trichoderma virens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.78 konjac glucomannan + H2O
-
 Trichoderma virens ?
-
 ?
3.2.1.78 konjac glucomannan + H2O
-
 Trichoderma virens UKM1 ?
-
 ?
3.2.1.78 locust bean gum + H2O
-
 Trichoderma virens ?
-
 ?
3.2.1.78 locust bean gum + H2O
-
 Trichoderma virens UKM1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.78 ? x * 65000-70000, His6-tagged enzyme, SDS-PAGE Trichoderma virens

Synonyms

EC Number Synonyms Comment Organism
3.2.1.78 endo-1,4-beta-mannanase
-
 Trichoderma virens
3.2.1.78 Man
-
 Trichoderma virens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.78 70
-
-
 Trichoderma virens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.78 70 80 the enzyme shows 70% of its maximum activity at 80°C Trichoderma virens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.78 20 70 the enzyme shows good stability along 20-65°C and retains more than 60% of its activity for up to 1 h at 60°C. The enzyme has a half-life of 26xa0min at 70°C Trichoderma virens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.78 5
-
-
 Trichoderma virens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.78 3 9 the enzyme has a wide pH stability range of pH 3.0-9.0 Trichoderma virens