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Literature summary extracted from

  • van Loo, B.; Schober, M.; Valkov, E.; Heberlein, M.; Bornberg-Bauer, E.; Faber, K.; Hyvoenen, M.; Hollfelder, F.
    Structural and mechanistic analysis of the choline sulfatase from Sinorhizobium melliloti A class I sulfatase specific for an alkyl sulfate ester (2018), J. Mol. Biol., 430, 1004-1023 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.6.6 expression in Escherichia coli Sinorhizobium meliloti

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.6.6 structure to 2.8 A resolution, shows a buried, largely hydrophobic active site. Conserved residue Glu386 plays a role in recognition of the quaternary ammonium group of the choline substrate Sinorhizobium meliloti

Protein Variants

EC Number Protein Variants Comment Organism
3.1.6.6 C54S mutation in active site residue, significant decrease in activity Sinorhizobium meliloti
3.1.6.6 E386L mutation in active site residue, significant decrease in activity Sinorhizobium meliloti
3.1.6.6 H104A mutation in active site residue, significant decrease in activity Sinorhizobium meliloti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.6.6 0.5
-
choline O-sulfate wild-type, pH 7.6, 25°C Sinorhizobium meliloti
3.1.6.6 2.1
-
phosphoryl choline wild-type, pH 7.6, 25°C Sinorhizobium meliloti
3.1.6.6 9.4
-
choline O-sulfate mutant C54S, pH 7.6, 25°C Sinorhizobium meliloti
3.1.6.6 13.3
-
choline O-sulfate mutant H104A, pH 7.6, 25°C Sinorhizobium meliloti
3.1.6.6 15
-
choline O-sulfate mutant E386L, pH 7.6, 25°C Sinorhizobium meliloti
3.1.6.6 19
-
4-nitrophenyl sulfate wild-type, pH 7.6, 25°C Sinorhizobium meliloti
3.1.6.6 50
-
4-nitrophenyl methylphosphate wild-type, pH 6.0, 25°C Sinorhizobium meliloti
3.1.6.6 67
-
4-nitrophenyl methylphosphonate wild-type, pH 6.0, 25°C Sinorhizobium meliloti

Organism

EC Number Organism UniProt Comment Textmining
3.1.6.6 Sinorhizobium meliloti O69787
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.6.6 4-nitrophenyl methylphosphate + H2O
-
Sinorhizobium meliloti 4-nitrophenol + methylphosphate
-
?
3.1.6.6 4-nitrophenyl methylphosphonate + H2O
-
Sinorhizobium meliloti 4-nitrophenol + phosphonate
-
?
3.1.6.6 4-nitrophenyl phosphate + H2O
-
Sinorhizobium meliloti 4-nitrophenol + phosphate
-
?
3.1.6.6 4-nitrophenyl sulfate + H2O
-
Sinorhizobium meliloti 4-nitrophenol + sulfate
-
?
3.1.6.6 choline O-sulfate + H2O
-
Sinorhizobium meliloti choline + sulfate
-
?
3.1.6.6 phosphoryl choline + H2O
-
Sinorhizobium meliloti choline + phosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
3.1.6.6 BetC
-
Sinorhizobium meliloti

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.6.6 0.004
-
4-nitrophenyl methylphosphate wild-type, pH 6.0, 25°C Sinorhizobium meliloti
3.1.6.6 0.011
-
choline O-sulfate mutant C54S, pH 7.6, 25°C Sinorhizobium meliloti
3.1.6.6 0.012
-
choline O-sulfate mutant E386L, pH 7.6, 25°C Sinorhizobium meliloti
3.1.6.6 0.027
-
phosphoryl choline wild-type, pH 7.6, 25°C Sinorhizobium meliloti
3.1.6.6 0.035
-
choline O-sulfate mutant H104A, pH 7.6, 25°C Sinorhizobium meliloti
3.1.6.6 0.1
-
4-nitrophenyl methylphosphonate wild-type, pH 6.0, 25°C Sinorhizobium meliloti
3.1.6.6 0.22
-
4-nitrophenyl sulfate wild-type, pH 7.6, 25°C Sinorhizobium meliloti
3.1.6.6 2.4
-
choline O-sulfate wild-type, pH 7.6, 25°C Sinorhizobium meliloti

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.6.6 0.00008
-
4-nitrophenyl methylphosphate wild-type, pH 6.0, 25°C Sinorhizobium meliloti
3.1.6.6 0.0008
-
choline O-sulfate mutant E386L, pH 7.6, 25°C Sinorhizobium meliloti
3.1.6.6 0.0012
-
choline O-sulfate mutant C54S, pH 7.6, 25°C Sinorhizobium meliloti
3.1.6.6 0.0015
-
4-nitrophenyl methylphosphonate wild-type, pH 6.0, 25°C Sinorhizobium meliloti
3.1.6.6 0.0026
-
choline O-sulfate mutant H104A, pH 7.6, 25°C Sinorhizobium meliloti
3.1.6.6 0.012
-
4-nitrophenyl sulfate wild-type, pH 7.6, 25°C Sinorhizobium meliloti
3.1.6.6 0.013
-
phosphoryl choline wild-type, pH 7.6, 25°C Sinorhizobium meliloti
3.1.6.6 4.8
-
choline O-sulfate wild-type, pH 7.6, 25°C Sinorhizobium meliloti