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Literature summary extracted from
- Saci_1816 A Trehalase that catalyzes trehalose degradation in the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius (2018), J. Microbiol. Biotechnol., 28, 909-916 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.28 | expression in Escherichia coli | Sulfolobus acidocaldarius |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.28 | 63600 | - |
recombinant enzyme, SDS-PAGE | Sulfolobus acidocaldarius |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.28 | Sulfolobus acidocaldarius | A0A2Z5U3T3 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.28 | - |
Sulfolobus acidocaldarius |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.28 | alpha,alpha-trehalose + H2O | - |
Sulfolobus acidocaldarius | beta-D-glucose + alpha-D-glucose | - |
? |  |
| 3.2.1.28 | additional information | the enzyme does not hydrolyze any other trehalose analogs such as maltose, nigerose, and turanose | Sulfolobus acidocaldarius | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.28 | Saci_1816 | locus name | Sulfolobus acidocaldarius |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.28 | 60 | - |
recombinant enzyme | Sulfolobus acidocaldarius |
| 3.2.1.28 | 80 | 85 | native enzyme | Sulfolobus acidocaldarius |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.28 | 40 | 75 | 40°C: about 75% of maximal activity, 75°C: about 35% of maximal activity, recombinant enzyme | Sulfolobus acidocaldarius |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.28 | additional information | - |
thermostability of recombinant enzyme is slightly reduced compared to native enzyme | Sulfolobus acidocaldarius |
| 3.2.1.28 | 90 | - |
half-life of native enzyme: 53 min, half-life of revcombinant enzyme: 41 min | Sulfolobus acidocaldarius |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.28 | 4 | - |
recombinant enzyme | Sulfolobus acidocaldarius |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.28 | 3 | 5 | pH 3.0: about 50% of maximal activity, pH 5.0: about 40% of maximal activity, recombinant enzyme | Sulfolobus acidocaldarius |
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Release 2023.1 (January 2023)
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