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Literature summary extracted from

  • Pauly, J.; Groeger, H.; Patel, A.V.
    Design, characterisation and application of alginate-based encapsulated pig liver esterase (2018), J. Biotechnol., 280, 42-48 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.1 synthesis application of encapsulated hydrolases for enzymatic syntheses Sus scrofa

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.1 additional information encapsulation of pig liver esterase, method development and optimization, overview. The coating of alginate and chitosan beads have been studied by creating various biopolymer hydrogel beads. Enzyme activity and encapsulation efficiency are notably enhanced by chitosan coating of alginate beads. Leaching is caused by the enzymatic reaction acidifying the matrix, which increases enzyme retention through enhanced electrostatic enzyme-alginate interaction but decreased activity through enzyme deactivation. A practical and ready-to-use method for visualising pH in beads during reaction by co-encapsulation of a conventional pH indicator is described including pH control inside the beads that can only be realised by buffering Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Sus scrofa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.1 commercial preparation
-
 Sus scrofa
-
3.1.1.1 liver
-
 Sus scrofa
-

Synonyms

EC Number Synonyms Comment Organism
3.1.1.1 esterase
-
 Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.1 22
-
 assay at room temperature Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 7.7
-
 assay at Sus scrofa