EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.3.27 | pgpB gene is cloned into the pET22b vector (Novagen) to generate the C-terminal His6-tagged protein. Mutants were generated using standard site-directed mutagenesis. The PgpB wild type and mutant proteins are expressed in Escherichia coli strain C41(DE3) | Escherichia coli |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.1.3.27 | vapor diffusion method, the structure is determined at 3.2 A resolution | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.3.27 | D211A | mutant enzyme retains 50% activity | Escherichia coli |
3.1.3.27 | H163A | mutation increases hydrolysis of 1,2-dipalmitoryl-sn-glycero-3-[phospho-rac-(1'-(3'-phospho)glycerol)] by 50% | Escherichia coli |
3.1.3.27 | K93A | the mutant enzyme shows complete activity loss for lysophosphatidic acid and (2S,3R,4E)-2-aminooctadec-4-ene-1,3-diol-1-phosphate, whereas it retains full activity for phosphatidic acid and gains 40% more activity toward phosphatidylglycerol phosphate compared with wild type enzyme | Escherichia coli |
3.1.3.27 | K93A/K97A | the mutant enzyme shows complete activity loss for lysophosphatidic acid and (2S,3R,4E)-2-aminooctadec-4-ene-1,3-diol-1-phosphate, whereas it retains full activity for phosphatidic acid and gained 40% more activity toward phosphatidylglycerol phosphate compared with wild type enzyme | Escherichia coli |
3.1.3.27 | K93T | mutant enzyme with significantly improved activity for lysophosphatidic acid, (2S,3R,4E)-2-aminooctadec-4-ene-1,3-diol-1-phosphate, and phosphatidic acid by 2.5- or 3.4-fold but not for 1,2-dipalmitoryl-sn-glycero-3-[phospho-rac-(1'-(3'-phospho)glycerol)] | Escherichia coli |
3.1.3.27 | K93T/K97A | the mutant enzyme loses all activity for lysophosphatidic acid or (2S,3R,4E)-2-aminooctadec-4-ene-1,3-diol-1-phosphate, but retains 50% activity for phosphatidic acid or 1,2-dipalmitoryl-sn-glycero-3-[phospho-rac-(1'-(3'-phospho)glycerol)] | Escherichia coli |
3.1.3.27 | K97A | the mutant enzyme shows complete activity loss for lysophosphatidic acid and (2S,3R,4E)-2-aminooctadec-4-ene-1,3-diol-1-phosphate, whereas it retains full activity for phosphatidic acid and gained 40% more activity toward phosphatidylglycerol phosphate compared with wild type enzyme | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.27 | orthovanadate | - |
Escherichia coli | |
3.1.3.27 | phosphatidylethanolamine | 3 mM, 70% inhibition. competitive | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.27 | 0.108 | - |
1-(9Z-octadecenoyl)-sn-glycero-3-phosphate | pH 7.5, 23°C | Escherichia coli | |
3.1.3.27 | 5.052 | - |
1-hexanoyl-sn-glycero-3-phosphate | pH 7.5, 23°C | Escherichia coli |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.3.27 | membrane | integral membrane protein | Escherichia coli | 16020 | - |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.27 | Escherichia coli | P0A924 | - |
- |
3.1.3.27 | Escherichia coli K12 | P0A924 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.3.27 | - |
Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.27 | (2S,3R,4E)-2-aminooctadec-4-ene-1,3-diol-1-phosphate + H2O | - |
Escherichia coli | ? | - |
? | |
3.1.3.27 | (2S,3R,4E)-2-aminooctadec-4-ene-1,3-diol-1-phosphate + H2O | - |
Escherichia coli K12 | ? | - |
? | |
3.1.3.27 | 1,2-dihexadecanoyl-sn-glycero-3-phosphate + H2O | - |
Escherichia coli | 1,2-dihexadecanoyl-sn-glycerol + phosphate | - |
? | |
3.1.3.27 | 1,2-dihexadecanoyl-sn-glycero-3-phosphate + H2O | - |
Escherichia coli K12 | 1,2-dihexadecanoyl-sn-glycerol + phosphate | - |
? | |
3.1.3.27 | 1,2-dipalmitoyl-sn-glycero-3-[phospho-rac-(1'-(3'-phospho)glycerol)] + H2O | - |
Escherichia coli | ? + phosphate | - |
? | |
3.1.3.27 | 1,2-dipalmitoyl-sn-glycero-3-[phospho-rac-(1'-(3'-phospho)glycerol)] + H2O | - |
Escherichia coli K12 | ? + phosphate | - |
? | |
3.1.3.27 | 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + H2O | - |
Escherichia coli | 1-(9Z-octadecenoyl)-sn-glycerol + phosphate | - |
? | |
3.1.3.27 | 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + H2O | - |
Escherichia coli K12 | 1-(9Z-octadecenoyl)-sn-glycerol + phosphate | - |
? | |
3.1.3.27 | 1-hexanoyl-sn-glycero-3-phosphate + H2O | - |
Escherichia coli | 1-hexanoyl-sn-glycerol + phosphate | - |
? | |
3.1.3.27 | 1-hexanoyl-sn-glycero-3-phosphate + H2O | - |
Escherichia coli K12 | 1-hexanoyl-sn-glycerol + phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.3.27 | PgpB | - |
Escherichia coli |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.27 | 2.7 | - |
phosphatidylethanolamine | pH 7.5, 23°C | Escherichia coli |