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Literature summary extracted from

  • Liu, L.; Komori, K.; Ishino, S.; Bocquier, A.; Cann, I.; Kohda, D.; Ishino, Y.
    The archaeal DNA primase Biochemical characterization of the p41-p46 complex from Pyrococcus furiosus (2001), J. Biol. Chem., 276, 45484-45490 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.102 ATP presence of ATP stimulates in vitro replication of M13 ssDNA Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.102 Pyrococcus furiosus Q9P9H1 and Q8U4H7 Q9P9H1, i.e. small subunit PriS, Q8U4H7, i.e. large subunit PriL
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.102 M13 ssDNA + n dNTP
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Pyrococcus furiosus M13 ssDNA/pppdN(pdN)n-1 + (n-1) diphosphate
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Synonyms

EC Number Synonyms Comment Organism
2.7.7.102 PriL
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Pyrococcus furiosus
2.7.7.102 PriS
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Pyrococcus furiosus

General Information

EC Number General Information Comment Organism
2.7.7.102 physiological function the complex between subunits PriS and PriL shows higher DNA binding activity than the catalytic PriS subunit alone. The amount of DNA synthesized by the complex is much more abundant and shorter in length than that by PriS alone. The activity for RNA primer synthesis is not detected with PriS alone but observed using the complex in vitro Pyrococcus furiosus