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Literature summary extracted from

  • Rowen, L.; Kornberg, A.
    Primase, the dnaG protein of Escherichia coli. An enzyme which starts DNA chains (1978), J. Biol. Chem., 253, 758-764 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.101 NaCl
-
 Escherichia coli
2.7.7.101 phosphate 0.04 mM, 40% inhibition Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.101 0.012
-
 rNTP pH 7.5, 30°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.101 Mg2+ presence of Mg2+ required, optimum concentration 2-8 mM Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.101 Escherichia coli P0ABS5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.101
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.101 1.09
-
 pH 7.5, 30°C Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.7.7.101 -180°C, stable for at least 1 year Escherichia coli
2.7.7.101 0°C, stable for months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.101 bacteriophage G4 ssDNA + n rNTP
-
 Escherichia coli bacteriophage G4 ssDNA/pppN(pN)n-1 hybrid + (n-1) diphosphate
-
 ?
2.7.7.101 additional information other single-stranded and duplex DNA templates tested are inactive Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.101 ? x * 60000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.101 30 37
-
 Escherichia coli

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.7.101 20
-
 about 50% of maximum activity Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.101 7.5
-
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.101 6.5 9
-
 Escherichia coli

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.7.101 50
-
 pH 7.5, 30°C Escherichia coli NaCl