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Literature summary extracted from

  • Burkhart, B.W.; Cubonova, L.; Heider, M.R.; Kelman, Z.; Reeve, J.N.; Santangelo, T.J.
    The GAN exonuclease or the Flap endonuclease Fen1 and RNase HII are necessary for viability of Thermococcus kodakarensis (2017), J. Bacteriol., 199, e00141-17 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
3.1.26.4 additional information construction of a gene TK0805 deletion strain lacking RNase HII activity. The mutant strain shows reduced growth compared to wild-type Thermococcus kodakarensis

Organism

EC Number Organism UniProt Comment Textmining
3.1.26.4 Thermococcus kodakarensis O74035
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Synonyms

EC Number Synonyms Comment Organism
3.1.26.4 ribonuclease HII
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 Thermococcus kodakarensis
3.1.26.4 RNase HII
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 Thermococcus kodakarensis
3.1.26.4 RnhB
-
 Thermococcus kodakarensis
3.1.26.4 TK0805
-
 Thermococcus kodakarensis

General Information

EC Number General Information Comment Organism
3.1.26.4 malfunction strains with both flap endonuclease (Fen1) and RNase HII deleted grow well. GINS-associated nuclease, GAN, activity is therefore sufficient for viability in the absence of both RNase HII and Fen1, but it is not possible to construct a strain with both RNase HII and GAN deleted. Fen1 alone is therefore insufficient for viability in the absence of both RNase HII and GAN. Deletion of both Fen1 and GAN or of both RNase HII and GAN is lethal Thermococcus kodakarensis
3.1.26.4 physiological function RNase HII is necessary for viability of Thermococcus kodakarensis. RNase HII is proposed to participate in primer processing during Okazaki fragment maturation. In Thermococcus kodakarensis, either flap endonuclease (Fen1) or GINS-associated nuclease (GAN) activity is sufficient for viability. GAN can support growth in the absence of both Fen1 and RNase HII, but Fen1 and RNase HII are required for viability in the absence of GAN. Individually, Fen1, GAN, and RNase HII are not essential for viability Thermococcus kodakarensis