EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.284 | expression in Escherichia coli | Mycobacterium tuberculosis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.284 | S141A | mutation in putative catalytic residue, loss of activity | Mycobacterium tuberculosis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.284 | tetrahydrolipstatin | tetrahydrolipstatin treatment of Mycobacterium tuberculosis results in selective inhibition of Chp2 over PapA3 | Mycobacterium tuberculosis |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.3.1.284 | cell envelope | - |
Mycobacterium tuberculosis | 30313 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.278 | a mycolipenoyl-CoA + a 2-(long-chain-fatty acyl)-trehalose | Mycobacterium tuberculosis | the enzyme participates in the biosynthesis of polyacyltrehalose, a pentaacylated, trehalose-based glycolipid found in the cell wall of pathogenic strains | a 2-(long-chain-fatty acyl)-3-mycolipenoyl-trehalose + CoA | - |
? | |
2.3.1.279 | a long-chain-fatty acyl-CoA + alpha,alpha-trehalose | Mycobacterium tuberculosis | the enzyme participates in the biosynthesis of polyacyltrehalose, a pentaacylated, trehalose-based glycolipid found in the cell wall of pathogenic strains | a 2-(long-chain-fatty acyl)-trehalose + CoA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.278 | Mycobacterium tuberculosis | P9WIK5 | - |
- |
2.3.1.279 | Mycobacterium tuberculosis | P9WIK5 | - |
- |
2.3.1.284 | Mycobacterium tuberculosis | O50440 | - |
- |
2.3.1.284 | Mycobacterium tuberculosis H37Rv | O50440 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.278 | - |
Mycobacterium tuberculosis |
2.3.1.279 | - |
Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.278 | a mycolipenoyl-CoA + a 2-(long-chain-fatty acyl)-trehalose | the enzyme participates in the biosynthesis of polyacyltrehalose, a pentaacylated, trehalose-based glycolipid found in the cell wall of pathogenic strains | Mycobacterium tuberculosis | a 2-(long-chain-fatty acyl)-3-mycolipenoyl-trehalose + CoA | - |
? | |
2.3.1.278 | a mycolipenoyl-CoA + a 2-(long-chain-fatty acyl)-trehalose | the enzyme catalyses two successive activities. It first transfers an acyl (often palmitoyl) group to position 2 (see EC 2.3.1.279, long-chain-acyl-CoA-trehalose acyltransferase), followed by the transfer of a mycolipenyl group to position 3 | Mycobacterium tuberculosis | a 2-(long-chain-fatty acyl)-3-mycolipenoyl-trehalose + CoA | - |
? | |
2.3.1.279 | a long-chain-fatty acyl-CoA + alpha,alpha-trehalose | the enzyme participates in the biosynthesis of polyacyltrehalose, a pentaacylated, trehalose-based glycolipid found in the cell wall of pathogenic strains | Mycobacterium tuberculosis | a 2-(long-chain-fatty acyl)-trehalose + CoA | - |
? | |
2.3.1.279 | a long-chain-fatty acyl-CoA + alpha,alpha-trehalose | the enzyme catalyses two successive activities. It first transfers an acyl (often palmitoyl) group to position 2, followed by the transfer of a mycolipenyl group to position 3 (see EC 2.3.1.278, mycolipenoyl-CoA-2-(long-chain-fatty acyl)-trehalose mycolipenoyltransferase) | Mycobacterium tuberculosis | a 2-(long-chain-fatty acyl)-trehalose + CoA | - |
? | |
2.3.1.284 | 3 3'-(hydroxy)phthioceranyl-2'-palmitoyl(stearoyl)-2-O-sulfo-alpha,alpha-trehalose | - |
Mycobacterium tuberculosis | 3,6,6'-tris-(hydroxy)phthioceranyl-2-palmitoyl(stearoyl)-2'-sulfo-alpha-alpha-trehalose + 2 2'-palmitoyl/stearoyl-2-O-sulfo-alpha,alpha-trehalose | - |
? | |
2.3.1.284 | 3 3'-(hydroxy)phthioceranyl-2'-palmitoyl(stearoyl)-2-O-sulfo-alpha,alpha-trehalose | - |
Mycobacterium tuberculosis H37Rv | 3,6,6'-tris-(hydroxy)phthioceranyl-2-palmitoyl(stearoyl)-2'-sulfo-alpha-alpha-trehalose + 2 2'-palmitoyl/stearoyl-2-O-sulfo-alpha,alpha-trehalose | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.278 | papA3 | - |
Mycobacterium tuberculosis |
2.3.1.279 | papA3 | - |
Mycobacterium tuberculosis |
2.3.1.284 | Chp2 | - |
Mycobacterium tuberculosis |
2.3.1.284 | diacyltrehalose acyltransferase | - |
Mycobacterium tuberculosis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.3.1.278 | metabolism | the enzyme participates in the biosynthesis of polyacyltrehalose, a pentaacylated, trehalose-based glycolipid found in the cell wall of pathogenic strains | Mycobacterium tuberculosis |
2.3.1.279 | metabolism | the enzyme participates in the biosynthesis of polyacyltrehalose, a pentaacylated, trehalose-based glycolipid found in the cell wall of pathogenic strains | Mycobacterium tuberculosis |
2.3.1.284 | physiological function | Chp2 is essential for the final steps of polyacyltrehalose biosynthesis. Disruption of Chp2 leads to the loss of polyacyltrehalose and accumulation of tetraacyltrehalose as well as the accumulation of diacyltrehalose, implicating Chp2 as an acyltransferase acting downstream of PapA3. Chp2 activity is probably potentiated by the DAT/PAT transporter MmpL10 | Mycobacterium tuberculosis |