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Literature summary extracted from

  • Touchette, M.H.; Holsclaw, C.M.; Previti, M.L.; Solomon, V.C.; Leary, J.A.; Bertozzi, C.R.; Seeliger, J.C.
    The rv1184c locus encodes Chp2, an acyltransferase in Mycobacterium tuberculosis polyacyltrehalose lipid biosynthesis (2015), J. Bacteriol., 197, 201-210 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.284 expression in Escherichia coli Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.284 S141A mutation in putative catalytic residue, loss of activity Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.284 tetrahydrolipstatin tetrahydrolipstatin treatment of Mycobacterium tuberculosis results in selective inhibition of Chp2 over PapA3 Mycobacterium tuberculosis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.284 cell envelope
-
Mycobacterium tuberculosis 30313
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.278 a mycolipenoyl-CoA + a 2-(long-chain-fatty acyl)-trehalose Mycobacterium tuberculosis the enzyme participates in the biosynthesis of polyacyltrehalose, a pentaacylated, trehalose-based glycolipid found in the cell wall of pathogenic strains a 2-(long-chain-fatty acyl)-3-mycolipenoyl-trehalose + CoA
-
?
2.3.1.279 a long-chain-fatty acyl-CoA + alpha,alpha-trehalose Mycobacterium tuberculosis the enzyme participates in the biosynthesis of polyacyltrehalose, a pentaacylated, trehalose-based glycolipid found in the cell wall of pathogenic strains a 2-(long-chain-fatty acyl)-trehalose + CoA
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.278 Mycobacterium tuberculosis P9WIK5
-
-
2.3.1.279 Mycobacterium tuberculosis P9WIK5
-
-
2.3.1.284 Mycobacterium tuberculosis O50440
-
-
2.3.1.284 Mycobacterium tuberculosis H37Rv O50440
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.278
-
Mycobacterium tuberculosis
2.3.1.279
-
Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.278 a mycolipenoyl-CoA + a 2-(long-chain-fatty acyl)-trehalose the enzyme participates in the biosynthesis of polyacyltrehalose, a pentaacylated, trehalose-based glycolipid found in the cell wall of pathogenic strains Mycobacterium tuberculosis a 2-(long-chain-fatty acyl)-3-mycolipenoyl-trehalose + CoA
-
?
2.3.1.278 a mycolipenoyl-CoA + a 2-(long-chain-fatty acyl)-trehalose the enzyme catalyses two successive activities. It first transfers an acyl (often palmitoyl) group to position 2 (see EC 2.3.1.279, long-chain-acyl-CoA-trehalose acyltransferase), followed by the transfer of a mycolipenyl group to position 3 Mycobacterium tuberculosis a 2-(long-chain-fatty acyl)-3-mycolipenoyl-trehalose + CoA
-
?
2.3.1.279 a long-chain-fatty acyl-CoA + alpha,alpha-trehalose the enzyme participates in the biosynthesis of polyacyltrehalose, a pentaacylated, trehalose-based glycolipid found in the cell wall of pathogenic strains Mycobacterium tuberculosis a 2-(long-chain-fatty acyl)-trehalose + CoA
-
?
2.3.1.279 a long-chain-fatty acyl-CoA + alpha,alpha-trehalose the enzyme catalyses two successive activities. It first transfers an acyl (often palmitoyl) group to position 2, followed by the transfer of a mycolipenyl group to position 3 (see EC 2.3.1.278, mycolipenoyl-CoA-2-(long-chain-fatty acyl)-trehalose mycolipenoyltransferase) Mycobacterium tuberculosis a 2-(long-chain-fatty acyl)-trehalose + CoA
-
?
2.3.1.284 3 3'-(hydroxy)phthioceranyl-2'-palmitoyl(stearoyl)-2-O-sulfo-alpha,alpha-trehalose
-
Mycobacterium tuberculosis 3,6,6'-tris-(hydroxy)phthioceranyl-2-palmitoyl(stearoyl)-2'-sulfo-alpha-alpha-trehalose + 2 2'-palmitoyl/stearoyl-2-O-sulfo-alpha,alpha-trehalose
-
?
2.3.1.284 3 3'-(hydroxy)phthioceranyl-2'-palmitoyl(stearoyl)-2-O-sulfo-alpha,alpha-trehalose
-
Mycobacterium tuberculosis H37Rv 3,6,6'-tris-(hydroxy)phthioceranyl-2-palmitoyl(stearoyl)-2'-sulfo-alpha-alpha-trehalose + 2 2'-palmitoyl/stearoyl-2-O-sulfo-alpha,alpha-trehalose
-
?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.278 papA3
-
Mycobacterium tuberculosis
2.3.1.279 papA3
-
Mycobacterium tuberculosis
2.3.1.284 Chp2
-
Mycobacterium tuberculosis
2.3.1.284 diacyltrehalose acyltransferase
-
Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
2.3.1.278 metabolism the enzyme participates in the biosynthesis of polyacyltrehalose, a pentaacylated, trehalose-based glycolipid found in the cell wall of pathogenic strains Mycobacterium tuberculosis
2.3.1.279 metabolism the enzyme participates in the biosynthesis of polyacyltrehalose, a pentaacylated, trehalose-based glycolipid found in the cell wall of pathogenic strains Mycobacterium tuberculosis
2.3.1.284 physiological function Chp2 is essential for the final steps of polyacyltrehalose biosynthesis. Disruption of Chp2 leads to the loss of polyacyltrehalose and accumulation of tetraacyltrehalose as well as the accumulation of diacyltrehalose, implicating Chp2 as an acyltransferase acting downstream of PapA3. Chp2 activity is probably potentiated by the DAT/PAT transporter MmpL10 Mycobacterium tuberculosis