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Literature summary extracted from

  • Zhang, Y.; Ji, J.; Zhao, X.; Jin, R.; Li, J.; Wan, H.
    Expression, purification and enzymatic properties of a beta-N-acetylhexosaminidase from brown planthopper, Nilaparvata lugens (2017), J. Asia Pac. Entomol., 20, 1340-1343 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.52 expressed in baculovirus-infected sf9 insect cells Nilaparvata lugens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 Ca2+ about 80% residual activity at 1 mM Nilaparvata lugens
3.2.1.52 Cd2+ about 85% residual activity at 1 mM Nilaparvata lugens
3.2.1.52 Co2+ about 90% residual activity at 1 mM Nilaparvata lugens
3.2.1.52 Cu2+ about 80% residual activity at 1 mM Nilaparvata lugens
3.2.1.52 E-64 about 70% residual activity at 1 mM Nilaparvata lugens
3.2.1.52 Fe2+ about 30% residual activity at 1 mM Nilaparvata lugens
3.2.1.52 Fe3+ about 55% residual activity at 1 mM Nilaparvata lugens
3.2.1.52 leupeptin about 90% residual activity at 1 mM Nilaparvata lugens
3.2.1.52 Mg2+ about 85% residual activity at 1 mM Nilaparvata lugens
3.2.1.52 Mn2+ about 85% residual activity at 1 mM Nilaparvata lugens
3.2.1.52 additional information not inhibited by aprotinin and trypsin Nilaparvata lugens
3.2.1.52 phenylmethanesulfonyl fluoride about 35% residual activity at 1 mM Nilaparvata lugens
3.2.1.52 Zn2+ about 85% residual activity at 1 mM Nilaparvata lugens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Nilaparvata lugens A0A0C5CE51 brown planthopper
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 MagneHis protein purification system Nilaparvata lugens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
-
 Nilaparvata lugens 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.52 ? x * 70000, SDS-PAGE Nilaparvata lugens

Synonyms

EC Number Synonyms Comment Organism
3.2.1.52 Hex4
-
 Nilaparvata lugens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.52 60
-
-
 Nilaparvata lugens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.52 30 70 about 45% activity at 30°C, about 75% activity at 40°C, about 95% activity at 50°C, 100% activity at 60°C, about 60% activity at 70°C Nilaparvata lugens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.52 50
-
 the recombinant enzyme exhibits thermal stability with more than 80-60% activity remaining at 50°C for at least 1 h Nilaparvata lugens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 6
-
-
 Nilaparvata lugens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.52 4 7 about 70% activity at pH 4.0, about 90% activity at pH 5.0, 100% activity at pH 6.0. about 70% activity at pH 7.0 Nilaparvata lugens